NealeLab
@labneale.bsky.social
Recombination, Meiosis, Chromosomes, DNA Break Repair
https://twitter.com/LabNeale
https://twitter.com/LabNeale
Matching T-shirt and slide @gdsc-sussex.bsky.social from Sir Paul Nurse
November 7, 2025 at 4:13 PM
Matching T-shirt and slide @gdsc-sussex.bsky.social from Sir Paul Nurse
Fantastic celebration of Tony Carr’s scientific career at the @gdsc-sussex.bsky.social retreat 2025
November 7, 2025 at 3:08 PM
Fantastic celebration of Tony Carr’s scientific career at the @gdsc-sussex.bsky.social retreat 2025
Good old @nathanwpyle.bsky.social 😀
October 27, 2025 at 12:46 PM
Good old @nathanwpyle.bsky.social 😀
Reminds me of The Starry Night upload.wikimedia.org/wikipedia/co...
September 28, 2025 at 1:47 PM
Reminds me of The Starry Night upload.wikimedia.org/wikipedia/co...
So utterly honoured to have been recruited, mentored and supported by this incredibly influential individual. Long may he happily retire. @gdsc-sussex.bsky.social
July 31, 2025 at 9:34 PM
So utterly honoured to have been recruited, mentored and supported by this incredibly influential individual. Long may he happily retire. @gdsc-sussex.bsky.social
Still computing...
Am I reading it correctly that the strongest *peak* signals detected (in trans) remain between "in-register" sister loop bases? (Circled)
It's the offset thing I'm not getting...
Could the pattern be indicative of the relative directionality of loop growth during replication?
Am I reading it correctly that the strongest *peak* signals detected (in trans) remain between "in-register" sister loop bases? (Circled)
It's the offset thing I'm not getting...
Could the pattern be indicative of the relative directionality of loop growth during replication?
July 17, 2025 at 11:24 AM
Still computing...
Am I reading it correctly that the strongest *peak* signals detected (in trans) remain between "in-register" sister loop bases? (Circled)
It's the offset thing I'm not getting...
Could the pattern be indicative of the relative directionality of loop growth during replication?
Am I reading it correctly that the strongest *peak* signals detected (in trans) remain between "in-register" sister loop bases? (Circled)
It's the offset thing I'm not getting...
Could the pattern be indicative of the relative directionality of loop growth during replication?
This looks (also) like a really interesting paper—thanks for sharing! We also saw dramatic remodelling of the chromosome upon GAL induction, but I don't think I'd describe it as a fountain. More extreme isolation: Fig 6b:
www.nature.com/articles/s41...
Note: Our HiC maps have origin bottom left.
www.nature.com/articles/s41...
Note: Our HiC maps have origin bottom left.
July 2, 2025 at 6:46 AM
This looks (also) like a really interesting paper—thanks for sharing! We also saw dramatic remodelling of the chromosome upon GAL induction, but I don't think I'd describe it as a fountain. More extreme isolation: Fig 6b:
www.nature.com/articles/s41...
Note: Our HiC maps have origin bottom left.
www.nature.com/articles/s41...
Note: Our HiC maps have origin bottom left.
Interesting...similar features are seen at S. cerevisiae point centromeres in G1 cells, but I guess these mammalian fountains are on a very different size scale?
July 1, 2025 at 12:46 PM
Interesting...similar features are seen at S. cerevisiae point centromeres in G1 cells, but I guess these mammalian fountains are on a very different size scale?
Getting ready for EMBO Meiosis 2025, Engelberg
June 22, 2025 at 8:28 AM
Getting ready for EMBO Meiosis 2025, Engelberg
Corsica - en route to phygenome.sciencesconf.org 🌊🐠🧬
May 31, 2025 at 7:11 PM
Corsica - en route to phygenome.sciencesconf.org 🌊🐠🧬
En route to #BMM2025 Liverpool organised by: @urszulamcclurg.bsky.social
March 26, 2025 at 7:44 AM
En route to #BMM2025 Liverpool organised by: @urszulamcclurg.bsky.social
Three generations of celebrations 🧑🎓👩🎓👨🎓
March 7, 2025 at 8:24 PM
Three generations of celebrations 🧑🎓👩🎓👨🎓
Such an honour to receive this gift from @genomedymanics.bsky.social
Inaugural cup of tea ☕️😍
Inaugural cup of tea ☕️😍
March 7, 2025 at 4:24 PM
Such an honour to receive this gift from @genomedymanics.bsky.social
Inaugural cup of tea ☕️😍
Inaugural cup of tea ☕️😍
Exploring Vienna after a fantastic visit here
February 15, 2025 at 9:02 AM
Exploring Vienna after a fantastic visit here
Now also added to the GDSC starter pack: go.bsky.app/D8e4DBe
December 10, 2024 at 10:47 PM
Now also added to the GDSC starter pack: go.bsky.app/D8e4DBe
We propose that chromatin perturbation remodels the accessibility to, and/or organisation of, the chromatin, enabling Top2 to resolve latent superhelical stresses (supercoils and plectonemes) that are otherwise absorbed and/or obscured by the inherent topological buffering capacity of chromatin:
December 5, 2024 at 3:07 PM
We propose that chromatin perturbation remodels the accessibility to, and/or organisation of, the chromatin, enabling Top2 to resolve latent superhelical stresses (supercoils and plectonemes) that are otherwise absorbed and/or obscured by the inherent topological buffering capacity of chromatin:
Such Top2 hotspots are observed across the genome, correlating with peaks in the transcriptional landscape, and are resolved within minutes:
December 5, 2024 at 3:02 PM
Such Top2 hotspots are observed across the genome, correlating with peaks in the transcriptional landscape, and are resolved within minutes:
...but within seconds of an acute chromatin perturbation, mediated by an acute osmotic shock, short-lived hotspots of Top2 activity emerge at sites of convergent transcription, long genes and transposon barriers—all locations that are predicted sites of positive superhelical stress:
December 5, 2024 at 3:00 PM
...but within seconds of an acute chromatin perturbation, mediated by an acute osmotic shock, short-lived hotspots of Top2 activity emerge at sites of convergent transcription, long genes and transposon barriers—all locations that are predicted sites of positive superhelical stress:
At steady state we capture Top2 activity across the genome with unprecedented detail and resolution—showing how it is distinctly patterned by gene organisation and chromatin (nucleosome) structure, but apparently not especially by transcriptional activity itself:
December 5, 2024 at 2:49 PM
At steady state we capture Top2 activity across the genome with unprecedented detail and resolution—showing how it is distinctly patterned by gene organisation and chromatin (nucleosome) structure, but apparently not especially by transcriptional activity itself:
Bit of post-3R&3C lab redecorating
December 4, 2024 at 1:24 PM
Bit of post-3R&3C lab redecorating