Kashish Singh
@kashishsingh.bsky.social
Postdoc @Carter_Lab @MRC_LMB. Alumnus of the Stark lab @mpi_nat.
Reposted by Kashish Singh
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
November 7, 2025 at 10:44 AM
An Asgard archaeon with internal membrane compartments
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!
www.biorxiv.org/content/10.1...
Reposted by Kashish Singh
Congrats🥳 to @mike-wieczorek.bsky.social, Bin Cai, and our former lab member Jingwei Xu @xujwet.bsky.social to their multiscale imaging 🔬 story of the centriolar AC linker #teamtomo
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Structure and assembly of the A-C linker connecting microtubule triplets in centrioles
Multiscale cryo-EM of centrioles reveals the structure and polymorphism of the A-C linker connecting microtubule triplets.
www.science.org
October 8, 2025 at 7:07 PM
Congrats🥳 to @mike-wieczorek.bsky.social, Bin Cai, and our former lab member Jingwei Xu @xujwet.bsky.social to their multiscale imaging 🔬 story of the centriolar AC linker #teamtomo
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Kashish Singh
The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...
The mechanistic basis of cargo selection during Golgi maturation
Resident proteins of the Golgi recycle in vesicles and the protein GOLPH3 enables the COPI vesicle coat to accomplish this.
www.science.org
October 6, 2025 at 11:39 AM
The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...
Reposted by Kashish Singh
TOMORROW – Nenad Ban from ETH Zürich will be joining us to discuss ‘Ribosome Upside Down: Co-translational Protein Biogenesis at the Tunnel Exit’.
Don’t miss this fantastic #LMBSeminar, beginning at 11am (BST).
More info: www2.mrc-lmb.cam.ac.uk/news-and-eve...
Don’t miss this fantastic #LMBSeminar, beginning at 11am (BST).
More info: www2.mrc-lmb.cam.ac.uk/news-and-eve...
September 16, 2025 at 10:04 AM
TOMORROW – Nenad Ban from ETH Zürich will be joining us to discuss ‘Ribosome Upside Down: Co-translational Protein Biogenesis at the Tunnel Exit’.
Don’t miss this fantastic #LMBSeminar, beginning at 11am (BST).
More info: www2.mrc-lmb.cam.ac.uk/news-and-eve...
Don’t miss this fantastic #LMBSeminar, beginning at 11am (BST).
More info: www2.mrc-lmb.cam.ac.uk/news-and-eve...
Reposted by Kashish Singh
LMB welcomes @sventruckenbrodt.bsky.social as a new Group Leader in our Neurobiology Division!
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
September 15, 2025 at 9:36 AM
LMB welcomes @sventruckenbrodt.bsky.social as a new Group Leader in our Neurobiology Division!
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
Reposted by Kashish Singh
Excited to share our new paper! 🎉
The BUB1 and BUBR1 paralogs scaffold the kinetochore fibrous corona
www.science.org/doi/10.1126/...
Very thankful to @andrea-musacchio.bsky.social for his mentorship and support over the years.
The BUB1 and BUBR1 paralogs scaffold the kinetochore fibrous corona
www.science.org/doi/10.1126/...
Very thankful to @andrea-musacchio.bsky.social for his mentorship and support over the years.
The BUB1 and BUBR1 paralogs scaffold the kinetochore fibrous corona
The kinetochore fibrous corona docks on CENP-E and a second elusive receptor identified as the checkpoint protein BUB1.
www.science.org
September 15, 2025 at 8:26 PM
Excited to share our new paper! 🎉
The BUB1 and BUBR1 paralogs scaffold the kinetochore fibrous corona
www.science.org/doi/10.1126/...
Very thankful to @andrea-musacchio.bsky.social for his mentorship and support over the years.
The BUB1 and BUBR1 paralogs scaffold the kinetochore fibrous corona
www.science.org/doi/10.1126/...
Very thankful to @andrea-musacchio.bsky.social for his mentorship and support over the years.
Reposted by Kashish Singh
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
August 31, 2025 at 6:44 PM
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social
www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
Reposted by Kashish Singh
Excited to see this work published online at @cp-cell.bsky.social today!
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
August 20, 2025 at 3:20 PM
Excited to see this work published online at @cp-cell.bsky.social today!
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Reposted by Kashish Singh
Sofia Lövestam's vault structure is now online in Structure @cellpress.bsky.social. 🥳
How beautifully explorative is #cryoEM! We never planned to do this. The vaults just came down with #amyloids from human brain tissue, and Sofia decided to have a closer look.
www.cell.com/structure/fu...
How beautifully explorative is #cryoEM! We never planned to do this. The vaults just came down with #amyloids from human brain tissue, and Sofia decided to have a closer look.
www.cell.com/structure/fu...
Cryo-EM structure of the vault from human brain reveals symmetry mismatch at its caps
Lövestam and Scheres present a cryo-EM structure of vault particles, found during
tau filament extraction from progressive supranuclear palsy (PSP) brain tissue. They
reveal a symmetry shift at the va...
www.cell.com
August 12, 2025 at 4:05 PM
Sofia Lövestam's vault structure is now online in Structure @cellpress.bsky.social. 🥳
How beautifully explorative is #cryoEM! We never planned to do this. The vaults just came down with #amyloids from human brain tissue, and Sofia decided to have a closer look.
www.cell.com/structure/fu...
How beautifully explorative is #cryoEM! We never planned to do this. The vaults just came down with #amyloids from human brain tissue, and Sofia decided to have a closer look.
www.cell.com/structure/fu...
Reposted by Kashish Singh
We’re looking for a Research Support Officer to join @matteoall.bsky.social’s group, working with @piotrkolata.bsky.social to investigate nuclear-cytoskeletal coupling in mammalian cells using #cryoEM, #cryoET & other biochemical approaches.
www.jobs.ac.uk/job/DOE317/r...
Closes 31 AUG
#ScienceJobs
www.jobs.ac.uk/job/DOE317/r...
Closes 31 AUG
#ScienceJobs
August 11, 2025 at 2:43 PM
We’re looking for a Research Support Officer to join @matteoall.bsky.social’s group, working with @piotrkolata.bsky.social to investigate nuclear-cytoskeletal coupling in mammalian cells using #cryoEM, #cryoET & other biochemical approaches.
www.jobs.ac.uk/job/DOE317/r...
Closes 31 AUG
#ScienceJobs
www.jobs.ac.uk/job/DOE317/r...
Closes 31 AUG
#ScienceJobs
Reposted by Kashish Singh
Our latest work, in collaboration with the terrific @kashishsingh.bsky.social in the Carter lab @mrclmb.bsky.social is out. So proud of this study and everyone involved...
Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization https://www.biorxiv.org/content/10.1101/2025.08.02.668268v1
August 5, 2025 at 8:13 AM
Our latest work, in collaboration with the terrific @kashishsingh.bsky.social in the Carter lab @mrclmb.bsky.social is out. So proud of this study and everyone involved...
Reposted by Kashish Singh
Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells
In eukaryotic cells, LINC complexes physically bridge the two nuclear membranes to cytoskeletal filaments, transmitting mechanical forces to the nucleus. Due to their dynamic and membrane-embedded nat...
www.biorxiv.org
August 5, 2025 at 6:14 AM
Fantastic work by the group of @matteoall.bsky.social : a SUN5:Nesprin3 complex at the head:tail junction of sperm cells. 🤯 #teamtomo
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Check out our latest work now out on biorxiv www.biorxiv.org/content/10.1...
Excited to share our latest work with @simonbullock11.bsky.social! We looked at how diverse mRNAs get selected for subcellular localization and it turns out that a single protein can recognize different RNA elements using shared features that weren’t apparent before.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
August 5, 2025 at 11:27 AM
Check out our latest work now out on biorxiv www.biorxiv.org/content/10.1...
Reposted by Kashish Singh
Check out this exciting new study from the @rgassmann.bsky.social lab that we are thrilled to be part of — revealing that CDR2 is a dynein adaptor that regulates ER sheet organization!!
Teixeira et al. @i3suporto.bsky.social identify the onconeural antigen CDR2 as an ER-localized adaptor for the microtubule-based motor cytoplasmic dynein-1 & show that CDR2 contributes to ER organization thru. its interaction w/ the integral ER membrane protein kinectin. rupress.org/jcb/article/...
July 11, 2025 at 3:28 PM
Check out this exciting new study from the @rgassmann.bsky.social lab that we are thrilled to be part of — revealing that CDR2 is a dynein adaptor that regulates ER sheet organization!!
Reposted by Kashish Singh
Join our Structural Studies Division, leading a research group developing computational methods to address questions in molecular & cellular biology!
You will be supported by core funding, excellent facilities & enthusiastic colleagues.
Apply by 18 MAR
www.nature.com/naturecareer...
You will be supported by core funding, excellent facilities & enthusiastic colleagues.
Apply by 18 MAR
www.nature.com/naturecareer...
March 3, 2025 at 10:05 AM
Join our Structural Studies Division, leading a research group developing computational methods to address questions in molecular & cellular biology!
You will be supported by core funding, excellent facilities & enthusiastic colleagues.
Apply by 18 MAR
www.nature.com/naturecareer...
You will be supported by core funding, excellent facilities & enthusiastic colleagues.
Apply by 18 MAR
www.nature.com/naturecareer...
Reposted by Kashish Singh
Today in #journalclub, we looked at this fantastic new method from the @doudna-lab.bsky.social in Nature Biotechnology! They show how CRISPR–Csm enables single-molecule live-cell imaging of endogenous RNAs. Super cool!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
February 21, 2025 at 12:56 PM
Today in #journalclub, we looked at this fantastic new method from the @doudna-lab.bsky.social in Nature Biotechnology! They show how CRISPR–Csm enables single-molecule live-cell imaging of endogenous RNAs. Super cool!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Kashish Singh
We are looking for a senior administrator to take care of the Structural Studies Division at @mrclmb.bsky.social. Come and work for the benefit of human health in a fantastic environment. Happy to chat!
www.jobs.ac.uk/job/DLY086/s...
www.jobs.ac.uk/job/DLY086/s...
Senior Administrator (Divisional Administrator) - Structural Studies - LMB 2565 at MRC Laboratory of Molecular Biology
Start your UK & international job search for academic jobs, research jobs, science jobs and managerial jobs in leading universities and top...
www.jobs.ac.uk
February 19, 2025 at 2:27 PM
We are looking for a senior administrator to take care of the Structural Studies Division at @mrclmb.bsky.social. Come and work for the benefit of human health in a fantastic environment. Happy to chat!
www.jobs.ac.uk/job/DLY086/s...
www.jobs.ac.uk/job/DLY086/s...
Reposted by Kashish Singh
The second of our two new preprints addresses the question of how proteasomes can be kept dormant for instance in oocytes. This study was spearheaded by first author Sascha Amann. biorxiv.org/cgi/content/... (1/6)🧵
PITHD1: An Endogenous Inhibitor of the 26S Proteasome During Cellular Dormancy
Cellular dormancy represents a state of regulated growth arrest essential for diverse biological processes, from reproduction to cancer progression. While mechanisms controlling protein synthesis in d...
biorxiv.org
December 6, 2024 at 9:17 AM
The second of our two new preprints addresses the question of how proteasomes can be kept dormant for instance in oocytes. This study was spearheaded by first author Sascha Amann. biorxiv.org/cgi/content/... (1/6)🧵
Reposted by Kashish Singh
A cryo-electron tomography study of ciliary rootlet organization pubmed.ncbi.nlm.nih.gov/39641991/
December 8, 2024 at 3:20 AM
A cryo-electron tomography study of ciliary rootlet organization pubmed.ncbi.nlm.nih.gov/39641991/
Reposted by Kashish Singh
If you’re interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), I’ve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. 🔬❄️🧪
November 27, 2024 at 12:15 PM
If you’re interested in how molecular motors coordinate to power intracellular trafficking events (e.g. endocytosis), I’ve got a PhD position available to study this at vibrant Bristol uni. In this project, we will capture highly dynamic events by #cryoEM, single molecule imaging & cell biology. 🔬❄️🧪
Reposted by Kashish Singh
We are very excited to share our recent preprint on the nuclear import mechanism of the proteasome, driven by the multivalent adaptor AKIRIN2! www.biorxiv.org/content/10.1... 🧵 1/6
A multivalent adaptor mechanism drives the nuclear import of proteasomes
Nuclear protein homeostasis, including the turnover of transcription factors, critically depends on nuclear proteasomes. After each cell division, proteasomes need to be re-imported into the newly for...
www.biorxiv.org
December 5, 2024 at 8:11 PM
We are very excited to share our recent preprint on the nuclear import mechanism of the proteasome, driven by the multivalent adaptor AKIRIN2! www.biorxiv.org/content/10.1... 🧵 1/6
Reposted by Kashish Singh
Our review of Intraflagellar transport is out now! We cover lots of the recent advances in our understanding of IFT, and where we think there are still some gaps.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
The intraflagellar transport cycle - Nature Reviews Molecular Cell Biology
Intraflagellar transport (IFT) ensures delivery of selected proteins into cilia. The IFT protein complexes IFT-A and IFT-B polymerize at the base of the cilium to form an anterograde train that facili...
www.nature.com
November 18, 2024 at 11:06 AM
Our review of Intraflagellar transport is out now! We cover lots of the recent advances in our understanding of IFT, and where we think there are still some gaps.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Kashish Singh
We have three PhD projects with motor-cytoskeleton theme though collaborations with three new(ish) PIs at Bristol Biochemistry supported by SWBio_DTP with Berni Carroll (lysosome transport), @abidalilab.bsky.social (endocytosis) and Jessica Cross (protein design)
see swbio.ac.uk/biomolecular...
see swbio.ac.uk/biomolecular...
Biomolecular and Biophysical Studies – SWBiosciences Doctoral Training Partnership
swbio.ac.uk
November 13, 2024 at 5:47 PM
We have three PhD projects with motor-cytoskeleton theme though collaborations with three new(ish) PIs at Bristol Biochemistry supported by SWBio_DTP with Berni Carroll (lysosome transport), @abidalilab.bsky.social (endocytosis) and Jessica Cross (protein design)
see swbio.ac.uk/biomolecular...
see swbio.ac.uk/biomolecular...