Jonathan Pruneda
@jnpruneda.bsky.social
Associate Professor at Oregon Health & Science University studying microbial interference in host ubiquitin signaling. Current ASM Northwest Branch President. Formerly @UWBiochemistry and @MRC_LMB.
Reposted by Jonathan Pruneda
Our new preprint is online! Viruses, bacteria and parasites use effector proteins to evade immunity and rewire host cell pathways. Together with @AlexanderStark8, we wondered if we could systematically map what these effectors, regardless of their origin, do in human cells. 1/8
www.biorxiv.org
November 18, 2025 at 3:57 PM
Our new preprint is online! Viruses, bacteria and parasites use effector proteins to evade immunity and rewire host cell pathways. Together with @AlexanderStark8, we wondered if we could systematically map what these effectors, regardless of their origin, do in human cells. 1/8
Reposted by Jonathan Pruneda
The second part of our (with @origichals.bsky.social and El Hadji) two-part review on non-covalent SUMO interactions is now published, focussing on SUMO-interacting motifs (SIMs), but discussing also other confirmed or potential SUMO-interacting elements.
Non-covalent SUMO interactions with ligases and effectors: SUMO-interacting motifs and beyond
SUMOylation, a protein post-translational modification (PTM) involving the covalent attachment of small ubiquitin-like modifier (SUMO), regulates a wide range of cellular processes. The key hallmark o...
portlandpress.com
November 24, 2025 at 9:06 PM
The second part of our (with @origichals.bsky.social and El Hadji) two-part review on non-covalent SUMO interactions is now published, focussing on SUMO-interacting motifs (SIMs), but discussing also other confirmed or potential SUMO-interacting elements.
Reposted by Jonathan Pruneda
So proud of the lab for its achievements - Thank you to the Pettenkofer foundation for recognising our contributions - here’s to some more bacterial effector related fun in the future! @dunnschool.bsky.social
Many congratulations Teresa!
Teresa Thurston was awarded the 2025 Pettenkofer Prize, recognising her contributions to identifying how different bacterial effectors impact the outcome of infection.
www.path.ox.ac.uk/news-article...
Teresa Thurston was awarded the 2025 Pettenkofer Prize, recognising her contributions to identifying how different bacterial effectors impact the outcome of infection.
www.path.ox.ac.uk/news-article...
2025 Pettenkofer Prize awarded to Teresa Thurston - Dunn School
The award by the Pettenkofer Foundation recognised Teresa’s contributions to identifying how different bacterial effectors impact the outcome of infection.
www.path.ox.ac.uk
November 23, 2025 at 9:43 PM
So proud of the lab for its achievements - Thank you to the Pettenkofer foundation for recognising our contributions - here’s to some more bacterial effector related fun in the future! @dunnschool.bsky.social
Reposted by Jonathan Pruneda
🚨 Postdoc position in my lab!
A project on protein binder discovery for K6-linked #ubiquitin to investigate their role in infection is available.
📍 DTU, Copenhagen, Denmark
⏰ Deadline: 18 December 2025
Apply here: efzu.fa.em2.oraclecloud.com/hcmUI/Candid...
A project on protein binder discovery for K6-linked #ubiquitin to investigate their role in infection is available.
📍 DTU, Copenhagen, Denmark
⏰ Deadline: 18 December 2025
Apply here: efzu.fa.em2.oraclecloud.com/hcmUI/Candid...
November 21, 2025 at 4:44 PM
🚨 Postdoc position in my lab!
A project on protein binder discovery for K6-linked #ubiquitin to investigate their role in infection is available.
📍 DTU, Copenhagen, Denmark
⏰ Deadline: 18 December 2025
Apply here: efzu.fa.em2.oraclecloud.com/hcmUI/Candid...
A project on protein binder discovery for K6-linked #ubiquitin to investigate their role in infection is available.
📍 DTU, Copenhagen, Denmark
⏰ Deadline: 18 December 2025
Apply here: efzu.fa.em2.oraclecloud.com/hcmUI/Candid...
Reposted by Jonathan Pruneda
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
Filament formation and NAD processing by noncanonical human FAM118 sirtuins
Nature Structural & Molecular Biology - Baretić and Missoury et al. identify vertebrate proteins FAM118B and FAM118A as sirtuins similar to bacterial antiphage enzymes and show that...
rdcu.be
November 17, 2025 at 11:37 AM
Very happy to share our collaborative project on FAM118 proteins - noncanonical sirtuins that form filaments and process NAD in human and other vertebrate cells.
Reposted by Jonathan Pruneda
⏳Four days to go until #AusUb2025! Check out the video below to hear some conference tips and tricks from our committee chair David Komander (and some potentially controversial interest rankings!?!?).
More interviews to come in the next few days so watch this space 😉
More interviews to come in the next few days so watch this space 😉
November 16, 2025 at 12:25 PM
⏳Four days to go until #AusUb2025! Check out the video below to hear some conference tips and tricks from our committee chair David Komander (and some potentially controversial interest rankings!?!?).
More interviews to come in the next few days so watch this space 😉
More interviews to come in the next few days so watch this space 😉
Reposted by Jonathan Pruneda
The Rowley/Ytreberg/Patel labs at UI have led a multidisciplinary study to characterize a new yeast toxin! Published in the ASM's flagship Journal - mBio. Eight undergraduate authors contributed to the study, a great team effort! journals.asm.org/doi/10.1128/... @uidaho.bsky.social
The Saccharomyces killer toxin K62 is a protein of the aerolysin family | mBio
Pore-forming toxins are potent biological weapons used across nature, from virulence
factors to immune defense proteins. This study identifies K62, a little-known antifungal
toxin produced by a wild y...
journals.asm.org
November 13, 2025 at 8:45 PM
The Rowley/Ytreberg/Patel labs at UI have led a multidisciplinary study to characterize a new yeast toxin! Published in the ASM's flagship Journal - mBio. Eight undergraduate authors contributed to the study, a great team effort! journals.asm.org/doi/10.1128/... @uidaho.bsky.social
Reposted by Jonathan Pruneda
ASM NW was wonderful this year! In addition to the great science, this trainee-focused meeting plays such an important role in providing the next generation of scientists, microbiologists, and clinicians an opportunity to learn, network, and share their own research.
🧪🦠
🧪🦠
November 12, 2025 at 4:55 PM
ASM NW was wonderful this year! In addition to the great science, this trainee-focused meeting plays such an important role in providing the next generation of scientists, microbiologists, and clinicians an opportunity to learn, network, and share their own research.
🧪🦠
🧪🦠
Reposted by Jonathan Pruneda
Our latest preprint is out!
We developed an inducible “RapTag” system that uses ENTAC to chemically recruit USP15 to the unstable protein CTLA4. This induced proximity stabilises and slows down the rapid lysosomal turnover of CTLA4.
@livuni-ismib.bsky.social
www.biorxiv.org/content/10.1...
We developed an inducible “RapTag” system that uses ENTAC to chemically recruit USP15 to the unstable protein CTLA4. This induced proximity stabilises and slows down the rapid lysosomal turnover of CTLA4.
@livuni-ismib.bsky.social
www.biorxiv.org/content/10.1...
Targeted recruitment of USP15 enhances CTLA4 surface levels and restricts its degradation.
Induced protein proximity offers powerful new routes to modulate protein fate. While proteolysis-targeting chimeras (PROTACs) promote degradation through E3 ligase recruitment, the converse principle-...
www.biorxiv.org
November 10, 2025 at 4:34 PM
Our latest preprint is out!
We developed an inducible “RapTag” system that uses ENTAC to chemically recruit USP15 to the unstable protein CTLA4. This induced proximity stabilises and slows down the rapid lysosomal turnover of CTLA4.
@livuni-ismib.bsky.social
www.biorxiv.org/content/10.1...
We developed an inducible “RapTag” system that uses ENTAC to chemically recruit USP15 to the unstable protein CTLA4. This induced proximity stabilises and slows down the rapid lysosomal turnover of CTLA4.
@livuni-ismib.bsky.social
www.biorxiv.org/content/10.1...
Reposted by Jonathan Pruneda
Excited to share our latest work, where we create Salmonella mutants deficient for cohorts of SPI-2 T3SS effectors. These strains can be used to efficiently identify particular effectors that contribute to SPI-2 phenotypes doi.org/10.1038/s414...
Salmonella multimutants enable efficient identification of SPI-2 effector protein function in gut inflammation and systemic colonization - Nature Communications
Intracellular Salmonella enterica uses a type III secretion system to secrete more than 30 effector proteins into host cells. Here, Newson et al. generate six mutant strains deficient in different set...
doi.org
November 10, 2025 at 8:02 AM
Excited to share our latest work, where we create Salmonella mutants deficient for cohorts of SPI-2 T3SS effectors. These strains can be used to efficiently identify particular effectors that contribute to SPI-2 phenotypes doi.org/10.1038/s414...
Reposted by Jonathan Pruneda
Can we improve peer review? We think we can! Check out an experiment I helped with as part of @solvingforsci.bsky.social 's mission to make science better.
And if you don't want to read the whole bioRxiv manuscript, here's an overview of the main findings:
solvingfor.org/news-posts-d...
And if you don't want to read the whole bioRxiv manuscript, here's an overview of the main findings:
solvingfor.org/news-posts-d...
November 5, 2025 at 11:25 PM
Can we improve peer review? We think we can! Check out an experiment I helped with as part of @solvingforsci.bsky.social 's mission to make science better.
And if you don't want to read the whole bioRxiv manuscript, here's an overview of the main findings:
solvingfor.org/news-posts-d...
And if you don't want to read the whole bioRxiv manuscript, here's an overview of the main findings:
solvingfor.org/news-posts-d...
Reposted by Jonathan Pruneda
SUMO interactions: the 1st of two reviews which Aanchal (@origichals.bsky.social), El Hadji, and I have recently written on non-covalent interactions of SUMO is now out. The 1st covers interactions with E1, E2, and deSUMOylating enzymes; the 2nd - on interactions with SIMs etc. - will appear soon.
Non-covalent SUMO interactions with (de)conjugation enzymes
SUMOylation – a protein post-translational modification (PTM) related to ubiquitylation – involves the reversible covalent attachment of the small ubiquitin-like modifier (SUMO) to proteins. During th...
portlandpress.com
November 5, 2025 at 1:44 PM
SUMO interactions: the 1st of two reviews which Aanchal (@origichals.bsky.social), El Hadji, and I have recently written on non-covalent interactions of SUMO is now out. The 1st covers interactions with E1, E2, and deSUMOylating enzymes; the 2nd - on interactions with SIMs etc. - will appear soon.
Reposted by Jonathan Pruneda
Have a look at this Tools of the Trade Article I wrote for @natrevmcb.nature.com on decoding #ubiquitin signals inside cells using UbiREAD!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
ICYMI: New Online! Decoding ubiquitin signals inside cells
Decoding ubiquitin signals inside cells
Nature Reviews Molecular Cell Biology, Published online: 31 October 2025; doi:10.1038/s41580-025-00919-zIn this Tools of the Trade article, Kiss (Schulman lab) discusses the development of UbiREAD, a method that delivers in vitro-ubiquitinated protein reporters into cells to systematically assess how different ubiquitin chain configurations affect protein stability and degradation.
bit.ly
November 4, 2025 at 10:04 AM
Have a look at this Tools of the Trade Article I wrote for @natrevmcb.nature.com on decoding #ubiquitin signals inside cells using UbiREAD!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Many thanks to @lisaheinke.bsky.social for the opportunity to write this TotT!
Reposted by Jonathan Pruneda
🚨Thrilled to share our latest work, published online today
@nature.com in which we decipher mechanisms underlying the enigmatic VEXAS syndrome - a huge team effort
@mskcancercenter.bsky.social led by superstars Varun Narendra + @tandriladas.bsky.social 1/
www.nature.com/articles/s41...
@nature.com in which we decipher mechanisms underlying the enigmatic VEXAS syndrome - a huge team effort
@mskcancercenter.bsky.social led by superstars Varun Narendra + @tandriladas.bsky.social 1/
www.nature.com/articles/s41...
Independent mechanisms of inflammation and myeloid bias in VEXAS syndrome - Nature
Nature - Independent mechanisms of inflammation and myeloid bias in VEXAS syndrome
www.nature.com
November 4, 2025 at 12:52 AM
🚨Thrilled to share our latest work, published online today
@nature.com in which we decipher mechanisms underlying the enigmatic VEXAS syndrome - a huge team effort
@mskcancercenter.bsky.social led by superstars Varun Narendra + @tandriladas.bsky.social 1/
www.nature.com/articles/s41...
@nature.com in which we decipher mechanisms underlying the enigmatic VEXAS syndrome - a huge team effort
@mskcancercenter.bsky.social led by superstars Varun Narendra + @tandriladas.bsky.social 1/
www.nature.com/articles/s41...
Reposted by Jonathan Pruneda
It's been an amazing year for the new PTM field of #MARUbylation - happy that I could get involved first-hand!
Excited to share the peer-reviewed version of our work, now online in @embojournal.org! Big thanks to our reviewers and @hvodermaier.bsky.social for facilitating this process. Looking forward to continuing this fun collaboration with @michaelnadbio.bsky.social!
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
November 3, 2025 at 1:42 PM
It's been an amazing year for the new PTM field of #MARUbylation - happy that I could get involved first-hand!
Reposted by Jonathan Pruneda
This October I’m drawing 1 molecule a day inspired by proteins in pdb @rcsbpdb.bsky.social
Day 20/31
Prompt RIVALS
Pdb: 8V27 & 8G6S
Two rival histone modifications on a nucleosome. Ubiquitination of H2BK120 activates genes while H2AK119 represses them.
Next: BLAST
Suggestions?
Day 20/31
Prompt RIVALS
Pdb: 8V27 & 8G6S
Two rival histone modifications on a nucleosome. Ubiquitination of H2BK120 activates genes while H2AK119 represses them.
Next: BLAST
Suggestions?
November 2, 2025 at 3:11 AM
This October I’m drawing 1 molecule a day inspired by proteins in pdb @rcsbpdb.bsky.social
Day 20/31
Prompt RIVALS
Pdb: 8V27 & 8G6S
Two rival histone modifications on a nucleosome. Ubiquitination of H2BK120 activates genes while H2AK119 represses them.
Next: BLAST
Suggestions?
Day 20/31
Prompt RIVALS
Pdb: 8V27 & 8G6S
Two rival histone modifications on a nucleosome. Ubiquitination of H2BK120 activates genes while H2AK119 represses them.
Next: BLAST
Suggestions?
Reposted by Jonathan Pruneda
The Wilkinson Lab is open for science! @mskcancercenter.bsky.social
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
Wilkinson Lab
We discover and study reverse transcriptases
wilkinsonlab.bio
October 31, 2025 at 7:00 PM
The Wilkinson Lab is open for science! @mskcancercenter.bsky.social
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
🧬We'll be finding funky new RNA biology, mainly by looking at reverse transcriptases (i.e. the Best Enzymes In The World)🧬
annnd: I'm hiring - come join! Especially postdocs and PhD students - please get in touch (NYC is great)
Reposted by Jonathan Pruneda
Our latest review is now online! 👇
@martenslab.bsky.social
Mechanism of autophagy initiation by transmembrane selective autophagy receptors | The EMBO Journal www.embopress.org/doi/full/10....
@martenslab.bsky.social
Mechanism of autophagy initiation by transmembrane selective autophagy receptors | The EMBO Journal www.embopress.org/doi/full/10....
Mechanism of autophagy initiation by transmembrane selective autophagy receptors | The EMBO Journal
EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.
www.embopress.org
October 31, 2025 at 6:24 AM
Our latest review is now online! 👇
@martenslab.bsky.social
Mechanism of autophagy initiation by transmembrane selective autophagy receptors | The EMBO Journal www.embopress.org/doi/full/10....
@martenslab.bsky.social
Mechanism of autophagy initiation by transmembrane selective autophagy receptors | The EMBO Journal www.embopress.org/doi/full/10....
Excited to share the peer-reviewed version of our work, now online in @embojournal.org! Big thanks to our reviewers and @hvodermaier.bsky.social for facilitating this process. Looking forward to continuing this fun collaboration with @michaelnadbio.bsky.social!
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
October 30, 2025 at 3:33 PM
Excited to share the peer-reviewed version of our work, now online in @embojournal.org! Big thanks to our reviewers and @hvodermaier.bsky.social for facilitating this process. Looking forward to continuing this fun collaboration with @michaelnadbio.bsky.social!
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
Reposted by Jonathan Pruneda
Exciting! Yet another Spotlight article has come out today in @cp-trendsparasitol.bsky.social, featuring our work on kinase-ligase crosstalk in a Leishmania virulence factor. Thanks so much, @jnpruneda.bsky.social and Shun-Je Bhark! www.sciencedirect.com/science/arti...
Practicing self-control: Leishmania TKUL exhibits intramolecular kinase-ubiquitin ligase crosstalk
Leishmaniasis, a neglected tropical disease caused by Leishmania parasites, poses an immense global health burden in desperate need of additional foun…
www.sciencedirect.com
October 29, 2025 at 1:44 PM
Exciting! Yet another Spotlight article has come out today in @cp-trendsparasitol.bsky.social, featuring our work on kinase-ligase crosstalk in a Leishmania virulence factor. Thanks so much, @jnpruneda.bsky.social and Shun-Je Bhark! www.sciencedirect.com/science/arti...
Reposted by Jonathan Pruneda
Job alert! We are recruiting a postdoc to study a novel RNA modification: ADP-ribose.
🔬Do you have experience working with RNA? Are you eager to explore new frontiers in RNA biology? Join our team and help uncover the secrets of RNA ADP-ribosylation: adpribosylationlab.wordpress.com/2025/10/28/p...
🔬Do you have experience working with RNA? Are you eager to explore new frontiers in RNA biology? Join our team and help uncover the secrets of RNA ADP-ribosylation: adpribosylationlab.wordpress.com/2025/10/28/p...
October 28, 2025 at 8:14 PM
Job alert! We are recruiting a postdoc to study a novel RNA modification: ADP-ribose.
🔬Do you have experience working with RNA? Are you eager to explore new frontiers in RNA biology? Join our team and help uncover the secrets of RNA ADP-ribosylation: adpribosylationlab.wordpress.com/2025/10/28/p...
🔬Do you have experience working with RNA? Are you eager to explore new frontiers in RNA biology? Join our team and help uncover the secrets of RNA ADP-ribosylation: adpribosylationlab.wordpress.com/2025/10/28/p...
Reposted by Jonathan Pruneda
The unique lipopolysaccharide composition of Salmonella Typhimurium ST313 dampens pyroptosis and inflammasome activation and suppresses host cell death https://www.biorxiv.org/content/10.1101/2025.09.29.679141v1
September 30, 2025 at 3:18 AM
The unique lipopolysaccharide composition of Salmonella Typhimurium ST313 dampens pyroptosis and inflammasome activation and suppresses host cell death https://www.biorxiv.org/content/10.1101/2025.09.29.679141v1
Reposted by Jonathan Pruneda
Come join us in Knoxville!
The Dept. of Microbiology 🧫🦠 at the University of Tennessee is hiring 2 Assistant Professors (tenure track, 9-month appt.).
Microbial Drivers of Chronic Disease: apply.interfolio.com/173153Quantitative Microbe-Plant-Environment Interactions: apply.interfolio.com/173345
The Dept. of Microbiology 🧫🦠 at the University of Tennessee is hiring 2 Assistant Professors (tenure track, 9-month appt.).
Microbial Drivers of Chronic Disease: apply.interfolio.com/173153Quantitative Microbe-Plant-Environment Interactions: apply.interfolio.com/173345
October 24, 2025 at 12:18 PM
Come join us in Knoxville!
The Dept. of Microbiology 🧫🦠 at the University of Tennessee is hiring 2 Assistant Professors (tenure track, 9-month appt.).
Microbial Drivers of Chronic Disease: apply.interfolio.com/173153Quantitative Microbe-Plant-Environment Interactions: apply.interfolio.com/173345
The Dept. of Microbiology 🧫🦠 at the University of Tennessee is hiring 2 Assistant Professors (tenure track, 9-month appt.).
Microbial Drivers of Chronic Disease: apply.interfolio.com/173153Quantitative Microbe-Plant-Environment Interactions: apply.interfolio.com/173345
Reposted by Jonathan Pruneda
UMN's Department of Microbiology and Immunology is hiring! Come be our colleague in the Twin Cities! Searches are open for Virology and Fungal Biology (links below).
Virology: hr.myu.umn.edu/jobs/ext/365...
Fungal Biology/Medical Mycology: hr.myu.umn.edu/jobs/ext/365...
Virology: hr.myu.umn.edu/jobs/ext/365...
Fungal Biology/Medical Mycology: hr.myu.umn.edu/jobs/ext/365...
October 26, 2025 at 7:40 PM
UMN's Department of Microbiology and Immunology is hiring! Come be our colleague in the Twin Cities! Searches are open for Virology and Fungal Biology (links below).
Virology: hr.myu.umn.edu/jobs/ext/365...
Fungal Biology/Medical Mycology: hr.myu.umn.edu/jobs/ext/365...
Virology: hr.myu.umn.edu/jobs/ext/365...
Fungal Biology/Medical Mycology: hr.myu.umn.edu/jobs/ext/365...