🌟 Why is this impactful? #Echidna doesn’t require joint scDNA/RNA sequencing (rare/expensive). Instead, it pairs scRNA-seq & WGS—readily obtainable from clinical samples. It’s scalable & can reveal mechanisms of cancer progression & treatment response! 🧬 6/

A Case Study in Melanoma: 🧬 Clones resistant to anti-PD-1 therapy showed clusters of phenotype-defining genes in hotspots of amplification! Including:
S100 family and MHC-II genes. #Echidna also disentangles intrinsic (CNA-driven) vs extrinsic expression immune signaling!

What did we find? Applied to tumor samples: 1️⃣ Clonal structure reconstructed with higher accuracy vs InferCNV/Numbat. 2️⃣ Temporal dynamics captured: tracks clonal evolution pre/post therapy. 3️⃣ Drivers of resistance identified using GDX in genomic hotspots. 4/

How it works: #Echidna models single-cell RNA & bulk WGS data using a Bayesian hierarchical framework. Key features:
Deconvolves CNA profiles
Tracks clones & their phenotypic states over time
Introduces GDX: a metric quantifying gene dosage effects! 3/

📌 Why Echidna? Phenotypic plasticity (cell adaptability) is critical in cancer progression & resistance. But how much of this plasticity is driven by gene dosage vs external cues?
Echidna uncouples these effects, bridging genome & transcriptome across timepoints!