Super congrats dear Richard

thank you dear luca for organizing this great meeting and having me. i had great time attending the fantastic diverse sessions and talks. I look forward for next one me and my lab embers would certainly become regular attendees.

12/n last one (plenty more but u should get the point by now) - iEFCs=60h in Preprint are shown to have a mix of PrE/Epi/TE , while in Paper they are homogeneously labeled in Magenta?

11/n in relation to the above concern, another panel of Paper in Fig.3F 32C ICM are marked (left) and according to this analysis on dataset used they also highly overlap with 16-Cell labeled in green? yet again why drown in scRNA-seq when immunostainings r so easy to solve this ?

10/n some meta-analysis was done to claim iEFC fall on 8 cell. Upon close inspection - 8C and 32ICM r coloured similarly! and there is NO arrow pointing to 32ICM on the UMAP. it seems 32ICM cells are nicely overlapping with iEFCs? where is 64cell ICM? But again, simple scRNA-seq above says it all!

9/n Straightforward scRNA-seq analysis of 60h=iEFC in Preprint also showed cells are not going backwards in development, but forward. Gata6+ cells express Sox17 and Pdgfra which are not 8-16cell markers but of good old E4.5 PrE cells.

8/n Strangely straightforward scRNA-seq analysis of 60h=iEFC in Preprint showing rare CDX2/GATA6 coexpression & and that iEFC is basically a mix of Epi/PrE/TE, was NOT included in the published Paper although same dataset was used in both. & Nanog is reduced in TE-&PrE-like cells.

7/n Preprint: 3/3 iEFC stainings show no/rare CDX2+/GATA6+ (include the same 2 above) and one in Preprint Fig1C, which was replaced in Paper Fig 1E with one that shows fuzzy coexpression. In sum 3 out of 4 immunostainings shown, rare CDX2/GATA6 co-expression!