Johannes Hohlbein
@hohlbeinlab.bsky.social
Single-molecule stuff, 🔬, 🦠 , 🧬, ☕, Assoc. Prof. @WUR (Laboratory of Biophysics, Wageningen), he/him, 🇩🇪->🇨🇿->🇩🇪->🇬🇧->🇳🇱, Pubs: https://scholar.google.nl/citations?hl=en&user=WZZAydwAAAAJ&view_op=list_works&sortby=pubdate
Pinned
First attempt in a (loosely defined) Single-Molecule Biophysics starter pack. Please share and (self-) nominate. go.bsky.app/CteFZM5
Reposted by Johannes Hohlbein
We are looking for a new technician 🥼 - initially limited with the option to a permanent position. If you are interested in molecular biology🧫, biochemistry🧬, a dynamic🕺, international🌍, welcoming science enthusiasts 🧑🔬 take a closer look👇 - Re-post appreciated www.verw.tu-dresden.de/StellAus/ste...
Vacancy ID 12501
Am Center for Molecular Bioengineering (B CUBE), einem Institut des Center for Molecular and Cellular Bioengineering (CMCB), ist an der Professur für Molekulare Biophysik ab sofort eine Stelle als
www.verw.tu-dresden.de
November 21, 2025 at 2:12 PM
We are looking for a new technician 🥼 - initially limited with the option to a permanent position. If you are interested in molecular biology🧫, biochemistry🧬, a dynamic🕺, international🌍, welcoming science enthusiasts 🧑🔬 take a closer look👇 - Re-post appreciated www.verw.tu-dresden.de/StellAus/ste...
Wait?! There are scientific 532nm and 561 nm diode lasers available these days? Direct analogue and digital modulation?! 🤯
November 21, 2025 at 2:59 PM
Wait?! There are scientific 532nm and 561 nm diode lasers available these days? Direct analogue and digital modulation?! 🤯
Friend of mine recently vibe-coded a small webapp for the Eisenhower principle (four-step prioritization tool). Code is open, feedback is welcome: s540d.github.io/Eisenhauer/
November 21, 2025 at 12:45 PM
Friend of mine recently vibe-coded a small webapp for the Eisenhower principle (four-step prioritization tool). Code is open, feedback is welcome: s540d.github.io/Eisenhauer/
Reposted by Johannes Hohlbein
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods
4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.
www.nature.com
November 21, 2025 at 3:00 AM
We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells.
www.nature.com/articles/s41...
rdcu.be/eQVxt
www.nature.com/articles/s41...
rdcu.be/eQVxt
Reposted by Johannes Hohlbein
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
November 20, 2025 at 11:42 AM
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
Reposted by Johannes Hohlbein
70 teaspoons placed in tearooms around the institute & observed weekly over 5 months. 80% of spoons disappeared; spoon halflife~81 days. Communal room halflife lower than in specific labs. 250 spoons annually required to maintain 70 spoon population.
pmc.ncbi.nlm.nih.gov/articles/PMC...
pmc.ncbi.nlm.nih.gov/articles/PMC...
The case of the disappearing teaspoons: longitudinal cohort study of the displacement of teaspoons in an Australian research institute
Objectives To determine the overall rate of loss of workplace teaspoons and whether attrition and displacement are correlated with the relative value of the teaspoons or type of tearoom. Design Longitudinal cohort study. Setting Research institute ...
pmc.ncbi.nlm.nih.gov
November 20, 2025 at 3:44 AM
70 teaspoons placed in tearooms around the institute & observed weekly over 5 months. 80% of spoons disappeared; spoon halflife~81 days. Communal room halflife lower than in specific labs. 250 spoons annually required to maintain 70 spoon population.
pmc.ncbi.nlm.nih.gov/articles/PMC...
pmc.ncbi.nlm.nih.gov/articles/PMC...
“Resilience requires inefficiency” and other takes from physics -> mental health. Interesting read: www.theguardian.com/commentisfre...
After I burned out, physics helped me understand what had happened to me – and to move on | Zahaan Bharmal
I thought hard work equalled success. I had to realise that’s not always how it works, in science or in life, says Google employee Zahaan Bharmal
www.theguardian.com
November 15, 2025 at 8:44 AM
“Resilience requires inefficiency” and other takes from physics -> mental health. Interesting read: www.theguardian.com/commentisfre...
Reposted by Johannes Hohlbein
4:35pm is an excellent time to teach the ins and outs of the harmonic oscillator. Followed by a computational tutorial until 6:55pm. :) #IteachPhysics
November 6, 2025 at 3:13 PM
4:35pm is an excellent time to teach the ins and outs of the harmonic oscillator. Followed by a computational tutorial until 6:55pm. :) #IteachPhysics
Reposted by Johannes Hohlbein
ICYMI – Check out our latest #preprint list on FocalPlane, highlighting the latest tools and techniques in microscopy.
focalplane.biologists.com/2025/10/22/m...
focalplane.biologists.com/2025/10/22/m...
Microscopy preprints – new tools and techniques in imaging - FocalPlane
Microscopy preprints – new tools and techniques in imaging - News
focalplane.biologists.com
October 27, 2025 at 4:27 PM
ICYMI – Check out our latest #preprint list on FocalPlane, highlighting the latest tools and techniques in microscopy.
focalplane.biologists.com/2025/10/22/m...
focalplane.biologists.com/2025/10/22/m...
Reposted by Johannes Hohlbein
🦠🔬🤖🧑💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training
📜 www.biorxiv.org/content/10.1...
📜 www.biorxiv.org/content/10.1...
October 22, 2025 at 11:10 AM
🦠🔬🤖🧑💻 #mAIcrobe is out! With @pinholab.bsky.social's lab, we launched an open-source framework for high-throughput bacterial image analysis. By rockstars A. Brito & B. Saraiva et al, making #DeepLearning for phenotyping accessible! Easy to use, plus model training
📜 www.biorxiv.org/content/10.1...
📜 www.biorxiv.org/content/10.1...
The good things are sometimes very close. Keep your eyes open.
October 4, 2025 at 2:15 PM
The good things are sometimes very close. Keep your eyes open.
Celebrated 2222 citations on Twitter. 3333 on BlueSky. 4444 on ???
October 1, 2025 at 5:43 PM
Celebrated 2222 citations on Twitter. 3333 on BlueSky. 4444 on ???
Reposted by Johannes Hohlbein
I have an online Eisenhower To-Do list where I copy & paste all completed tasks to the bottom of the list. Eisenhower: 1) important and urgent (Do!), 2) important but not urgent (Schedule!), 3) not important but urgent (Delegate!), 4) not important not urgent (Ignore!)
October 1, 2025 at 11:56 AM
I have an online Eisenhower To-Do list where I copy & paste all completed tasks to the bottom of the list. Eisenhower: 1) important and urgent (Do!), 2) important but not urgent (Schedule!), 3) not important but urgent (Delegate!), 4) not important not urgent (Ignore!)
Reposted by Johannes Hohlbein
Our 6-hands review/perspective: "Hacking 3D printers as laboratory robots" is now on ChemRxiv.
In there, we show how many robots you can make by simply modifying a 3D printer. #chemsky 1/n
with @sandersays.bsky.social and @supersciencegrl.co.uk
chemrxiv.org/engage/chemr...
In there, we show how many robots you can make by simply modifying a 3D printer. #chemsky 1/n
with @sandersays.bsky.social and @supersciencegrl.co.uk
chemrxiv.org/engage/chemr...
September 30, 2025 at 2:07 PM
Our 6-hands review/perspective: "Hacking 3D printers as laboratory robots" is now on ChemRxiv.
In there, we show how many robots you can make by simply modifying a 3D printer. #chemsky 1/n
with @sandersays.bsky.social and @supersciencegrl.co.uk
chemrxiv.org/engage/chemr...
In there, we show how many robots you can make by simply modifying a 3D printer. #chemsky 1/n
with @sandersays.bsky.social and @supersciencegrl.co.uk
chemrxiv.org/engage/chemr...
Reposted by Johannes Hohlbein
Picard management tip: Some of your decisions are going to be mistakes. You have to make them anyway.
September 28, 2025 at 3:04 PM
Picard management tip: Some of your decisions are going to be mistakes. You have to make them anyway.
Picard is always right, fellow scientists.
Picard management tip: Even without game-changing results, experimentation is time well spent.
September 26, 2025 at 8:54 PM
Picard is always right, fellow scientists.
Friend got me some Corsican chestnut flour. Turned out rather well. #sourdough
September 21, 2025 at 5:45 PM
Friend got me some Corsican chestnut flour. Turned out rather well. #sourdough
Shower thought: instead of paying $$$ for article processing charges and open access, why don’t I/we preprint and pay two reviewers $ to improve the manuscript?
September 15, 2025 at 7:32 PM
Shower thought: instead of paying $$$ for article processing charges and open access, why don’t I/we preprint and pay two reviewers $ to improve the manuscript?
Reposted by Johannes Hohlbein
An diejenigen unter euch, die selbst in der Wissenschaft tätig sind:
'Dinge, von denen ich mir wünsche, ich hätte sie früher gewusst' - was wäre eure Antwort gewesen?
'Dinge, von denen ich mir wünsche, ich hätte sie früher gewusst' - was wäre eure Antwort gewesen?
September 13, 2025 at 6:19 AM
An diejenigen unter euch, die selbst in der Wissenschaft tätig sind:
'Dinge, von denen ich mir wünsche, ich hätte sie früher gewusst' - was wäre eure Antwort gewesen?
'Dinge, von denen ich mir wünsche, ich hätte sie früher gewusst' - was wäre eure Antwort gewesen?
Reposted by Johannes Hohlbein
Please share: The application for Les Houches is open (until Oct 31)
fluorescenceleshouches.wordpress.com
*This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!*
PS: no fees this year, you just need to get there, that's all!
fluorescenceleshouches.wordpress.com
*This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!*
PS: no fees this year, you just need to get there, that's all!
French-German WE-Heraeus-Seminar "Fluorescence markers for advanced microscopy : From photophysics to biology - 2026"
March 15-20 2026, Les Houches, France
fluorescenceleshouches.wordpress.com
September 12, 2025 at 9:03 AM
Please share: The application for Les Houches is open (until Oct 31)
fluorescenceleshouches.wordpress.com
*This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!*
PS: no fees this year, you just need to get there, that's all!
fluorescenceleshouches.wordpress.com
*This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!*
PS: no fees this year, you just need to get there, that's all!
Reposted by Johannes Hohlbein
After being frustrated for several years with struggling to build electronics-intensive microfluidic setup, that finally led to development of the significant part of control electronics by myself, I realized it would be nice to develop some kind of #microfluidics hardware development kit, 🧵
September 9, 2025 at 12:26 PM
After being frustrated for several years with struggling to build electronics-intensive microfluidic setup, that finally led to development of the significant part of control electronics by myself, I realized it would be nice to develop some kind of #microfluidics hardware development kit, 🧵
Puzzles me why one has to scroll to the bottom of the webpage to resolve a DOI at www.doi.org.
September 9, 2025 at 7:56 AM
Puzzles me why one has to scroll to the bottom of the webpage to resolve a DOI at www.doi.org.
Reposted by Johannes Hohlbein
Dear scientific community,
I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.
my.corehr.com/pls/uoxrecru...
my.corehr.com/pls/uoxrecru...
I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.
my.corehr.com/pls/uoxrecru...
my.corehr.com/pls/uoxrecru...
September 8, 2025 at 3:16 PM
Dear scientific community,
I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.
my.corehr.com/pls/uoxrecru...
my.corehr.com/pls/uoxrecru...
I am looking for two postdoctoral structural biologists to join my group at the Department of Biochemistry, University of Oxford.
my.corehr.com/pls/uoxrecru...
my.corehr.com/pls/uoxrecru...
Reposted by Johannes Hohlbein
Another academic year, another NWO Physics of Life newsletter!
Check it out 👇
nwobiophysics.nl/newsletters
> Interview with former chair Kristina Ganzinger (AMOLF) and our new chair Kristin Grußmayer @tudelfttnw.bsky.social
> Biosketch by Lisa Tran @utrechtuniversity.bsky.social
> and more..
Check it out 👇
nwobiophysics.nl/newsletters
> Interview with former chair Kristina Ganzinger (AMOLF) and our new chair Kristin Grußmayer @tudelfttnw.bsky.social
> Biosketch by Lisa Tran @utrechtuniversity.bsky.social
> and more..
September 4, 2025 at 2:55 PM
Another academic year, another NWO Physics of Life newsletter!
Check it out 👇
nwobiophysics.nl/newsletters
> Interview with former chair Kristina Ganzinger (AMOLF) and our new chair Kristin Grußmayer @tudelfttnw.bsky.social
> Biosketch by Lisa Tran @utrechtuniversity.bsky.social
> and more..
Check it out 👇
nwobiophysics.nl/newsletters
> Interview with former chair Kristina Ganzinger (AMOLF) and our new chair Kristin Grußmayer @tudelfttnw.bsky.social
> Biosketch by Lisa Tran @utrechtuniversity.bsky.social
> and more..