Heilemann Lab
@heilemannlab.bsky.social
Single-molecule biophysics group at Goethe University Frankfurt/Germany. Super-resolution microscopy. Optical structural cell biology. Computational microscopy. Posts by MH.
http://www.smb.uni-frankfurt.de
#superresolution, #livecellimaging, #imageanalysis
http://www.smb.uni-frankfurt.de
#superresolution, #livecellimaging, #imageanalysis
Pinned
Heilemann Lab
@heilemannlab.bsky.social
· Dec 13
#FluorescenceFriday (we are watching vimentin (HaloTag7) in U2OS cells co-stained with Nile Red) #livecell #longterm
🚨🔬A great opportunity to work with high-end microscopy in an exciting scientific environment. Please re-post 🙏 @goetheuni.bsky.social @scale-cluster-ffm.bsky.social
👇🏻
👇🏻
A great opportunity to join our newly established imaging centre in Frankfurt as engineer/technician. If you love light microscopy, please apply!
Please share!
Please share!
November 20, 2025 at 4:33 PM
🚨🔬A great opportunity to work with high-end microscopy in an exciting scientific environment. Please re-post 🙏 @goetheuni.bsky.social @scale-cluster-ffm.bsky.social
👇🏻
👇🏻
Reposted by Heilemann Lab
Physical principles have redefined our understanding of autophagy.
In our new @cp-trendsbiochem.bsky.social review, we discuss how phase separation, avidity, and wetting shape autophagy at the molecular level.
Great collaboration with @wilflinglab.bsky.social
www.cell.com/trends/bioch...
In our new @cp-trendsbiochem.bsky.social review, we discuss how phase separation, avidity, and wetting shape autophagy at the molecular level.
Great collaboration with @wilflinglab.bsky.social
www.cell.com/trends/bioch...
Autophagy regulation by phase separation, avidity, and wetting
Autophagy enables cells to selectively degrade a wide range of macromolecules, and
how this process achieves spatial precision within the densely packed cytosol is an
active area of investigation. Rec...
www.cell.com
November 20, 2025 at 1:34 PM
Physical principles have redefined our understanding of autophagy.
In our new @cp-trendsbiochem.bsky.social review, we discuss how phase separation, avidity, and wetting shape autophagy at the molecular level.
Great collaboration with @wilflinglab.bsky.social
www.cell.com/trends/bioch...
In our new @cp-trendsbiochem.bsky.social review, we discuss how phase separation, avidity, and wetting shape autophagy at the molecular level.
Great collaboration with @wilflinglab.bsky.social
www.cell.com/trends/bioch...
Reposted by Heilemann Lab
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Heilemann Lab
🧪How does an ABC transporter 'know' nucleotides & substrates bound to far apart binding sites? Using 1H15N, 19F NMR, HDX-MS, PET-FCS & activity assays with Neuweiler, Marcoux, Orelle & Jault labs, we found a communication hinge! 😍🥳
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
Bidirectional communication between nucleotide and substrate binding sites in a type IV multidrug ABC transporter
Nature Communications - The communication hinge, found in type IV ABC transporters, forms a bidirectional communication between ATP- and substrate-binding sites. This is an unexplored allosteric...
rdcu.be
November 12, 2025 at 11:15 AM
🧪How does an ABC transporter 'know' nucleotides & substrates bound to far apart binding sites? Using 1H15N, 19F NMR, HDX-MS, PET-FCS & activity assays with Neuweiler, Marcoux, Orelle & Jault labs, we found a communication hinge! 😍🥳
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
@microverse.bsky.social @lifeprofile.bsky.social
rdcu.be/ePm4C
Reposted by Heilemann Lab
Reposted by Heilemann Lab
In two weeks, our #qbio #Master students learned to build, from drawing to full realization, their own : confocal FCS, TIRF microscope, and optical tweezers setup ! This is hand-on #microscopy !
Interested ? Join the qbio master next year at the Montpellier University !
qbio.umontpellier.fr
Interested ? Join the qbio master next year at the Montpellier University !
qbio.umontpellier.fr
November 8, 2025 at 11:45 AM
In two weeks, our #qbio #Master students learned to build, from drawing to full realization, their own : confocal FCS, TIRF microscope, and optical tweezers setup ! This is hand-on #microscopy !
Interested ? Join the qbio master next year at the Montpellier University !
qbio.umontpellier.fr
Interested ? Join the qbio master next year at the Montpellier University !
qbio.umontpellier.fr
Reposted by Heilemann Lab
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 6, 2025 at 5:20 PM
🤯This is wow! Molecular mechanism of mRNA export. by @juliusbrennecke.bsky.social @plaschkalab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Heilemann Lab
🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️
D-CHAB @ethz.ch
www.nature.com/articles/s41...
D-CHAB @ethz.ch
www.nature.com/articles/s41...
Synthetic fluorophores for live-cell fluorescence microscopy and biosensing - Nature Chemical Biology
Performing live-cell microscopy experiments with high spatial and temporal resolution requires fluorophores with highly optimized properties. This Review examines the progress in developing synthetic ...
www.nature.com
November 5, 2025 at 9:29 PM
🚨Curious how synthetic fluorophores simplify microscopy? Our review covers applications such as super-resolution microscopy and biosensing. 🎉👩🔬Congrats to my first PhD student, Agnese Nicoli, for the fearless deep dive! 🔬 ⚗️
D-CHAB @ethz.ch
www.nature.com/articles/s41...
D-CHAB @ethz.ch
www.nature.com/articles/s41...
Reposted by Heilemann Lab
🔬🤖 ICYMI: impressive preprint from @owenlabuob.bsky.social tackling a major bottleneck – how to systematically compare super-resolution microscopy across experiments. Novel ML framework embeds single-molecule data for cross-sample analysis. Integrated with nano-org database✨ doi.org/10.1101/2025...
November 3, 2025 at 5:57 PM
🔬🤖 ICYMI: impressive preprint from @owenlabuob.bsky.social tackling a major bottleneck – how to systematically compare super-resolution microscopy across experiments. Novel ML framework embeds single-molecule data for cross-sample analysis. Integrated with nano-org database✨ doi.org/10.1101/2025...
Reposted by Heilemann Lab
Single-molecule friends!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
2026 Single Molecule Approaches to Biology Conference GRC
The 2026 Gordon Research Conference on Single Molecule Approaches to Biology will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.
www.grc.org
November 2, 2025 at 7:23 PM
Single-molecule friends!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
The prelim. program of next year's sm- #Gordon conference in Switzerland is online: www.grc.org/single-molec...
It'll be 3x fantastic: science, people, place in the alps ⛰️. Kevin & I hope to see you all next June:
Have a look, tell your friends, and register!
Reposted by Heilemann Lab
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
October 31, 2025 at 9:24 AM
📢 Open Call! The Max Perutz Labs invite applications for a Full Professorship in Integrative Structure Biology with a focus on in situ structural biology using cryo-electron tomography (cryo-ET) and related methods. More details ➡️ tinyurl.com/brswbymu
👇🏻 great opportunity for a PhD, great environment & PI!
🚨 #PhD Opportunities 🚨
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
🧵
How does plasma membrane structure underpin tissue development?
Let’s find out!
Opportunity to do a PhD with me, co-supervised by Darius Koester, at Warwick.
Fully funded studentship by #MIBTP.
🔬✨🐟
More info:
www.findaphd.com/phds/project...
Interested? Reach out!
November 1, 2025 at 8:25 AM
👇🏻 great opportunity for a PhD, great environment & PI!
Reposted by Heilemann Lab
High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodes - @pku1898.bsky.social go.nature.com/47yENVJ
High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodes - Nature Biotechnology
The 64-plex fluorescence imaging of RNA in tissues is achieved in one imaging cycle
go.nature.com
October 30, 2025 at 4:02 PM
High-plex spatial RNA imaging in one round with conventional microscopes using color-intensity barcodes - @pku1898.bsky.social go.nature.com/47yENVJ
Reposted by Heilemann Lab
Excited to share my first PhD student’s @biorxivpreprint.bsky.social!
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
November 1, 2025 at 5:56 AM
Excited to share my first PhD student’s @biorxivpreprint.bsky.social!
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Tracking 5 dyes simultaneously Kavan Gor @embl.org tracks nascent #RNA folding during #ribosome assembly to correlate structural with functional information on single RNA molecules!
Check it out!
www.biorxiv.org/content/10.1...
Reposted by Heilemann Lab
Tracking has always been at the ❤️ of the scientific method, from planetary motion to single particles.
Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.
Take a look! 😊
www.aip.org/scilights/tr...
Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.
Take a look! 😊
www.aip.org/scilights/tr...
Tracing fluorescence tracking’s past, present, and future
Artificial intelligence and parallel computing could refine studies of the movement of single molecules inside cells.
www.aip.org
October 30, 2025 at 11:19 PM
Tracking has always been at the ❤️ of the scientific method, from planetary motion to single particles.
Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.
Take a look! 😊
www.aip.org/scilights/tr...
Weiqing’s thesis intro on tracking was so beautiful we turned it into a perspective in JCP who then selected it as a #Scilight.
Take a look! 😊
www.aip.org/scilights/tr...
Reposted by Heilemann Lab
1/ Excited to share our new study with @brumbaugh-lab.bsky.social, out in @natbiotech.nature.com! P-bodies selectively sequester RNAs encoding cell fate regulators, often from the preceding developmental stage. Releasing these RNAs can drive changes in cell identity. 🧵 www.nature.com/articles/s41...
Selective RNA sequestration in biomolecular condensates directs cell fate transitions - Nature Biotechnology
Stem cell differentiation is controlled by manipulating RNA condensates.
www.nature.com
October 28, 2025 at 4:02 PM
1/ Excited to share our new study with @brumbaugh-lab.bsky.social, out in @natbiotech.nature.com! P-bodies selectively sequester RNAs encoding cell fate regulators, often from the preceding developmental stage. Releasing these RNAs can drive changes in cell identity. 🧵 www.nature.com/articles/s41...
Reposted by Heilemann Lab
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
October 27, 2025 at 9:43 PM
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
Reposted by Heilemann Lab
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells
Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....
onlinelibrary.wiley.com
October 25, 2025 at 8:56 AM
Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....
Reposted by Heilemann Lab
🚀🔬 Announcing #IMS2026 at @itqbnova.bsky.social! We're assembling a #LifeSciences #Microscopy symposium packed with ✨ speakers and 🧑🔬 workshops. All about imaging cells with photons, electrons and AI! If you love microscopy, you need to be here. Join us on March 19!
ims2026.itqbnovacommunity.org
ims2026.itqbnovacommunity.org
October 21, 2025 at 2:25 PM
🚀🔬 Announcing #IMS2026 at @itqbnova.bsky.social! We're assembling a #LifeSciences #Microscopy symposium packed with ✨ speakers and 🧑🔬 workshops. All about imaging cells with photons, electrons and AI! If you love microscopy, you need to be here. Join us on March 19!
ims2026.itqbnovacommunity.org
ims2026.itqbnovacommunity.org
👇🏻opportunity!
🚀 Exciting Job Opportunity: Project Coordinator at the Cluster of Excellence SCALE
We’re thrilled to announce an exceptional opportunity to join the newly founded Cluster of Excellence SCALE – Subcellular Architecture of Life from the @goetheuni.bsky.social.
👉 Learn more and apply here:
We’re thrilled to announce an exceptional opportunity to join the newly founded Cluster of Excellence SCALE – Subcellular Architecture of Life from the @goetheuni.bsky.social.
👉 Learn more and apply here:
October 22, 2025 at 4:32 PM
👇🏻opportunity!
Reposted by Heilemann Lab
We are recuiting two new Associate Professors here in Oxford Biochemistry. Come join us! Reach out to me if you have any questions. Please repost! tinyurl.com/mr3m7bd3
October 17, 2025 at 2:56 PM
We are recuiting two new Associate Professors here in Oxford Biochemistry. Come join us! Reach out to me if you have any questions. Please repost! tinyurl.com/mr3m7bd3
Very much recommended winter school!👇🏻🔬
Please repost. Only a few days left to apply to the “Ecole de Physique des Houches” on Fluorescence Markers for Advanced Microscopies, a WE-Heraeus seminar. Deadline October 31th.
fluorescenceleshouches.wordpress.com
#microscopy #fluorescence #superresolution
fluorescenceleshouches.wordpress.com
#microscopy #fluorescence #superresolution
October 17, 2025 at 6:01 PM
Very much recommended winter school!👇🏻🔬
Reposted by Heilemann Lab
And it's out!
Our paper on the optimization of smFISH to quantify cytokine mRNAs in T cells is published in EMBO Journal.
Using this protocol, we gathered interesting insights on cytokine mRNAs co-expression and localization in these teeny tiny human cells.
www.embopress.org/doi/full/10....
Our paper on the optimization of smFISH to quantify cytokine mRNAs in T cells is published in EMBO Journal.
Using this protocol, we gathered interesting insights on cytokine mRNAs co-expression and localization in these teeny tiny human cells.
www.embopress.org/doi/full/10....
October 15, 2025 at 9:31 PM
And it's out!
Our paper on the optimization of smFISH to quantify cytokine mRNAs in T cells is published in EMBO Journal.
Using this protocol, we gathered interesting insights on cytokine mRNAs co-expression and localization in these teeny tiny human cells.
www.embopress.org/doi/full/10....
Our paper on the optimization of smFISH to quantify cytokine mRNAs in T cells is published in EMBO Journal.
Using this protocol, we gathered interesting insights on cytokine mRNAs co-expression and localization in these teeny tiny human cells.
www.embopress.org/doi/full/10....
Reposted by Heilemann Lab
❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...
cryoTIGER: deep-learning based tilt interpolation generator for enhanced reconstruction in cryo electron tomography - Communications Biology
Deep learning-based interpolation using cryoTIGER enhances angular sampling in cryoET, improving 3D reconstructions, particle localization, and structural recovery without increasing electron dose.
www.nature.com
October 10, 2025 at 10:35 AM
❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...
Reposted by Heilemann Lab
We are hiring ! If you want to become professor of Bioinformatics in the best city of North America, check the announcement below ! Also you do need to speak French (as long as you commit to learning it :) ) www.umontreal.ca/public/www/d...
www.umontreal.ca
October 10, 2025 at 11:48 AM
We are hiring ! If you want to become professor of Bioinformatics in the best city of North America, check the announcement below ! Also you do need to speak French (as long as you commit to learning it :) ) www.umontreal.ca/public/www/d...