Gabriel Negreira
@gnegreira.bsky.social
#Leishmaniac, #FWO junior postdoc at @itmantwerp.bsky.social.
Using #singlecellgenomics to understand Aneuploidy and Genome instability in #Leishmania.
Using #singlecellgenomics to understand Aneuploidy and Genome instability in #Leishmania.
This is just the beginning! We hope this method will help the community to explore genome evolution and parasite adaptation in Leishmania and beyond!
For more info, check the preprint in @biorxivpreprint.bsky.social:
www.biorxiv.org/content/10.1...
For more info, check the preprint in @biorxivpreprint.bsky.social:
www.biorxiv.org/content/10.1...
A novel high-throughput single-cell DNA sequencing method reveals hidden genomic heterogeneity in the unicellular eukaryote Leishmania
Genome instability is considered a major driver of adaptation in eukaryotic microorganisms, but its study is hampered by the limited availability of genomic technologies with single-cell resolution. H...
www.biorxiv.org
September 11, 2025 at 10:15 AM
This is just the beginning! We hope this method will help the community to explore genome evolution and parasite adaptation in Leishmania and beyond!
For more info, check the preprint in @biorxivpreprint.bsky.social:
www.biorxiv.org/content/10.1...
For more info, check the preprint in @biorxivpreprint.bsky.social:
www.biorxiv.org/content/10.1...
(6/6)
We also revealed a previously unknown population heterogeneity in a #Leishmania strain which is commonly used in laboratory research.
We also revealed a previously unknown population heterogeneity in a #Leishmania strain which is commonly used in laboratory research.
September 11, 2025 at 10:15 AM
(6/6)
We also revealed a previously unknown population heterogeneity in a #Leishmania strain which is commonly used in laboratory research.
We also revealed a previously unknown population heterogeneity in a #Leishmania strain which is commonly used in laboratory research.
(5/6)
In our latest preprint, we combined Semi-permeable capsules ( #SPCs ) with #PTA to sequence the DNA of individual #Leishmania parasites at high throughput, identifying multiple layers of genetic heterogeneity, from mosaic aneuploidy to variations in the sequence of drug-resistance genes.
In our latest preprint, we combined Semi-permeable capsules ( #SPCs ) with #PTA to sequence the DNA of individual #Leishmania parasites at high throughput, identifying multiple layers of genetic heterogeneity, from mosaic aneuploidy to variations in the sequence of drug-resistance genes.
September 11, 2025 at 10:15 AM
(5/6)
In our latest preprint, we combined Semi-permeable capsules ( #SPCs ) with #PTA to sequence the DNA of individual #Leishmania parasites at high throughput, identifying multiple layers of genetic heterogeneity, from mosaic aneuploidy to variations in the sequence of drug-resistance genes.
In our latest preprint, we combined Semi-permeable capsules ( #SPCs ) with #PTA to sequence the DNA of individual #Leishmania parasites at high throughput, identifying multiple layers of genetic heterogeneity, from mosaic aneuploidy to variations in the sequence of drug-resistance genes.
(4/6)
However, the Chromium single-cell CNV solution was discontinued in 2020, and since then, the filed has been left without viable alternatives.
www.10xgenomics.com/products/sin...
However, the Chromium single-cell CNV solution was discontinued in 2020, and since then, the filed has been left without viable alternatives.
www.10xgenomics.com/products/sin...
Single Cell CNV | 10x Genomics
Chromium Single Cell CNV provides a comprehensive, scalable solution for revealing genome heterogeneity and understanding clonal evolution. Study disease pathogenesis or characterize neuronal mosaicis...
www.10xgenomics.com
September 11, 2025 at 10:15 AM
(4/6)
However, the Chromium single-cell CNV solution was discontinued in 2020, and since then, the filed has been left without viable alternatives.
www.10xgenomics.com/products/sin...
However, the Chromium single-cell CNV solution was discontinued in 2020, and since then, the filed has been left without viable alternatives.
www.10xgenomics.com/products/sin...
(3/6)
We also used it to understand how karyotype diversity (mosaic aneuploidy) could help parasites to survive drugs that in principle should kill them:
www.embopress.org/doi/full/10....
We also used it to understand how karyotype diversity (mosaic aneuploidy) could help parasites to survive drugs that in principle should kill them:
www.embopress.org/doi/full/10....
The adaptive roles of aneuploidy and polyclonality in Leishmania in response to environmental stress | EMBO reports
image image The dynamics of aneuploidy, a hallmark of Leishmania adaptation, depends on the nature and strength of the environmental stress as well
as on the existence of other pre‐adaptive mechanism...
www.embopress.org
September 11, 2025 at 10:15 AM
(3/6)
We also used it to understand how karyotype diversity (mosaic aneuploidy) could help parasites to survive drugs that in principle should kill them:
www.embopress.org/doi/full/10....
We also used it to understand how karyotype diversity (mosaic aneuploidy) could help parasites to survive drugs that in principle should kill them:
www.embopress.org/doi/full/10....
(2/6)
Later, we used the Chromium Single-cell CNV solution (10X Genomic) to determine the chromosome configuration (karyotypes) of thousands of Leishmania parasites, which led to some hypothesis on how karyotypes evolve in culture.
academic.oup.com/nar/article/...
Later, we used the Chromium Single-cell CNV solution (10X Genomic) to determine the chromosome configuration (karyotypes) of thousands of Leishmania parasites, which led to some hypothesis on how karyotypes evolve in culture.
academic.oup.com/nar/article/...
High throughput single-cell genome sequencing gives insights into the generation and evolution of mosaic aneuploidy in Leishmania donovani
Abstract. Leishmania, a unicellular eukaryotic parasite, is a unique model for aneuploidy and cellular heterogeneity, along with their potential role in ad
academic.oup.com
September 11, 2025 at 10:15 AM
(2/6)
Later, we used the Chromium Single-cell CNV solution (10X Genomic) to determine the chromosome configuration (karyotypes) of thousands of Leishmania parasites, which led to some hypothesis on how karyotypes evolve in culture.
academic.oup.com/nar/article/...
Later, we used the Chromium Single-cell CNV solution (10X Genomic) to determine the chromosome configuration (karyotypes) of thousands of Leishmania parasites, which led to some hypothesis on how karyotypes evolve in culture.
academic.oup.com/nar/article/...
(1/6)
More than 5 years ago we embarked on the journey of using #scDNAseq to study genetic heterogeneity in #Leishmania.
www.nature.com/articles/s41...
More than 5 years ago we embarked on the journey of using #scDNAseq to study genetic heterogeneity in #Leishmania.
www.nature.com/articles/s41...
Evaluation of whole genome amplification and bioinformatic methods for the characterization of Leishmania genomes at a single cell level - Scientific Reports
Scientific Reports - Evaluation of whole genome amplification and bioinformatic methods for the characterization of Leishmania genomes at a single cell level
www.nature.com
September 11, 2025 at 10:15 AM
(1/6)
More than 5 years ago we embarked on the journey of using #scDNAseq to study genetic heterogeneity in #Leishmania.
www.nature.com/articles/s41...
More than 5 years ago we embarked on the journey of using #scDNAseq to study genetic heterogeneity in #Leishmania.
www.nature.com/articles/s41...