Ryan Fink
@finkryan.bsky.social
PhD Student - Universität Bremen
and Universität zu Köln @massecklab.bsky.social
Check out our new calcium sensor PinkyCaMP: doi.org/10.1101/2024.12.16.628673
Now on Addgene!
and Universität zu Köln @massecklab.bsky.social
Check out our new calcium sensor PinkyCaMP: doi.org/10.1101/2024.12.16.628673
Now on Addgene!
Reposted by Ryan Fink
Abolish ICE.
There’s no reforming it.
There’s no compromise.
There’s only one way to rein in ICE’s terror campaign. Abolish it.
There’s no reforming it.
There’s no compromise.
There’s only one way to rein in ICE’s terror campaign. Abolish it.
February 2, 2026 at 11:27 PM
Abolish ICE.
There’s no reforming it.
There’s no compromise.
There’s only one way to rein in ICE’s terror campaign. Abolish it.
There’s no reforming it.
There’s no compromise.
There’s only one way to rein in ICE’s terror campaign. Abolish it.
Reposted by Ryan Fink
Great to see this officially out: An mTurquoise2-Based Glucose Biosensor - works in yeast & mammalian cells and is compatible with FLIM: pubs.acs.org/doi/10.1021/...
An mTurquoise2-Based Glucose Biosensor
Glucose is an important substrate for organisms to acquire energy needed for cellular growth. Despite the importance of this metabolite, single-cell information at a fast time-scale about the dynamics...
pubs.acs.org
February 3, 2026 at 9:10 PM
Great to see this officially out: An mTurquoise2-Based Glucose Biosensor - works in yeast & mammalian cells and is compatible with FLIM: pubs.acs.org/doi/10.1021/...
Reposted by Ryan Fink
Today I'd like to honor the memory of my mentor and friend, Roger Tsien, born 1952 February 1. Today would have been Roger's 74th birthday.
Most know Roger for his 2008 Chemistry Nobel Prize with Shimomura and Chalfie. Roger made GFP into the versatile imaging method it is now.
Most know Roger for his 2008 Chemistry Nobel Prize with Shimomura and Chalfie. Roger made GFP into the versatile imaging method it is now.
February 1, 2026 at 10:51 PM
Today I'd like to honor the memory of my mentor and friend, Roger Tsien, born 1952 February 1. Today would have been Roger's 74th birthday.
Most know Roger for his 2008 Chemistry Nobel Prize with Shimomura and Chalfie. Roger made GFP into the versatile imaging method it is now.
Most know Roger for his 2008 Chemistry Nobel Prize with Shimomura and Chalfie. Roger made GFP into the versatile imaging method it is now.
Reposted by Ryan Fink
An engineered nanobody inhibitor for molecular-to-circuit control of opioid receptor function. This was a really fun collaboration with @miriamstoeber.bsky.social and @amanglik.bsky.social labs, check it out! www.biorxiv.org/content/10.6...
www.biorxiv.org
January 18, 2026 at 12:23 PM
An engineered nanobody inhibitor for molecular-to-circuit control of opioid receptor function. This was a really fun collaboration with @miriamstoeber.bsky.social and @amanglik.bsky.social labs, check it out! www.biorxiv.org/content/10.6...
Reposted by Ryan Fink
We've got ISSUES. Literally.
We scraped >100k special issues & over 1 million articles to bring you a PISS-poor paper. We quantify just how many excess papers are published by guest editors abusing special issues to boost their CVs. How bad is it & what can we do?
arxiv.org/abs/2601.07563
A 🧵 1/n
We scraped >100k special issues & over 1 million articles to bring you a PISS-poor paper. We quantify just how many excess papers are published by guest editors abusing special issues to boost their CVs. How bad is it & what can we do?
arxiv.org/abs/2601.07563
A 🧵 1/n
January 13, 2026 at 8:27 AM
We've got ISSUES. Literally.
We scraped >100k special issues & over 1 million articles to bring you a PISS-poor paper. We quantify just how many excess papers are published by guest editors abusing special issues to boost their CVs. How bad is it & what can we do?
arxiv.org/abs/2601.07563
A 🧵 1/n
We scraped >100k special issues & over 1 million articles to bring you a PISS-poor paper. We quantify just how many excess papers are published by guest editors abusing special issues to boost their CVs. How bad is it & what can we do?
arxiv.org/abs/2601.07563
A 🧵 1/n
Reposted by Ryan Fink
In this mesmerizing video by our #ElectronMicroscopy team, a layer 5 thin tufted neuron is revealed with blue dendrites and purple axon. As it progresses, we zoom into where it connects to a pyramidal cell.
Explore our EM data at: microns-explorer.org
Explore our EM data at: microns-explorer.org
January 7, 2026 at 11:52 PM
In this mesmerizing video by our #ElectronMicroscopy team, a layer 5 thin tufted neuron is revealed with blue dendrites and purple axon. As it progresses, we zoom into where it connects to a pyramidal cell.
Explore our EM data at: microns-explorer.org
Explore our EM data at: microns-explorer.org
Reposted by Ryan Fink
The 'Version of Record' of our paper came out on #FluorescenceFriday
elifesciences.org/articles/105...
elifesciences.org/articles/105...
A green lifetime biosensor for calcium that remains bright over its full dynamic range
Directed evolution was used to engineer a green fluorescent biosensor for quantitative calcium imaging that remains fluorescent, yet shows a large change in lifetime in cells.
elifesciences.org
December 19, 2025 at 6:26 PM
The 'Version of Record' of our paper came out on #FluorescenceFriday
elifesciences.org/articles/105...
elifesciences.org/articles/105...
Reposted by Ryan Fink
December 5, 2025 at 1:21 PM
Reposted by Ryan Fink
Fresh batch of Hot Plasmids here! Find new tools for:
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
November 11, 2025 at 3:00 PM
Fresh batch of Hot Plasmids here! Find new tools for:
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
Reposted by Ryan Fink
What happens when you combine 10 years of brain data with one of the world’s fastest supercomputers?
A virtual mouse cortex simulation, thanks to a global collaboration.
🧠📈 https://alleninstitute.org/news/one-of-worlds-most-detailed-virtual-brain-simulations-is-changing-how-we-study-the-brain/
A virtual mouse cortex simulation, thanks to a global collaboration.
🧠📈 https://alleninstitute.org/news/one-of-worlds-most-detailed-virtual-brain-simulations-is-changing-how-we-study-the-brain/
November 17, 2025 at 7:26 PM
What happens when you combine 10 years of brain data with one of the world’s fastest supercomputers?
A virtual mouse cortex simulation, thanks to a global collaboration.
🧠📈 https://alleninstitute.org/news/one-of-worlds-most-detailed-virtual-brain-simulations-is-changing-how-we-study-the-brain/
A virtual mouse cortex simulation, thanks to a global collaboration.
🧠📈 https://alleninstitute.org/news/one-of-worlds-most-detailed-virtual-brain-simulations-is-changing-how-we-study-the-brain/
Reposted by Ryan Fink
mStayRose is published in JBC 🥳🎉
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
StayRose: a photostable StayGold derivative red-shifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality
images in microscopy. StayGold, a novel dimeric green fluorescent protein recently
monomerised through sequence engi...
www.jbc.org
October 22, 2025 at 4:58 PM
mStayRose is published in JBC 🥳🎉
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Reposted by Ryan Fink
New week, new tool: Find our Protein Domain Designer tool to generate publication-ready protein domain diagrams here: domaindesigner.farnunglab.com
September 29, 2025 at 3:01 PM
New week, new tool: Find our Protein Domain Designer tool to generate publication-ready protein domain diagrams here: domaindesigner.farnunglab.com
Dying in the weather, but the views in Seoul are incredible
August 29, 2025 at 5:03 AM
Dying in the weather, but the views in Seoul are incredible
Very excited to be at the #KSBNS2025 in a few days!
I’ll be presenting a poster in the first session (P-097) about our mScarlet based sensor for calcium PinkyCaMP and our developing mScarlet and 5HT1A based serotonin sensor.
Stop by to talk and learn about this new generation of red sensors!
I’ll be presenting a poster in the first session (P-097) about our mScarlet based sensor for calcium PinkyCaMP and our developing mScarlet and 5HT1A based serotonin sensor.
Stop by to talk and learn about this new generation of red sensors!
August 21, 2025 at 12:59 PM
Very excited to be at the #KSBNS2025 in a few days!
I’ll be presenting a poster in the first session (P-097) about our mScarlet based sensor for calcium PinkyCaMP and our developing mScarlet and 5HT1A based serotonin sensor.
Stop by to talk and learn about this new generation of red sensors!
I’ll be presenting a poster in the first session (P-097) about our mScarlet based sensor for calcium PinkyCaMP and our developing mScarlet and 5HT1A based serotonin sensor.
Stop by to talk and learn about this new generation of red sensors!
Reposted by Ryan Fink
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
August 19, 2025 at 7:31 PM
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
Reposted by Ryan Fink
#OCaMP is now on bioRxiv and reagents are available on #Addgene! 🧪
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
www.biorxiv.org/content/10.1...
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
www.biorxiv.org/content/10.1...
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance...
www.biorxiv.org
July 31, 2025 at 7:34 PM
#OCaMP is now on bioRxiv and reagents are available on #Addgene! 🧪
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
www.biorxiv.org/content/10.1...
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
www.biorxiv.org/content/10.1...
Reposted by Ryan Fink
For those of you attending academic conferences in the U.S., after October 1, 2025 please note the following:
Tucked away in the 'One Big Beautiful Bill' is the requirement that all visitors to the US must pay $250 just to enter the country starting 1 October! For a family of 5 that's $1250! I doubt this will help America's crashing tourism business which is down 10% already since January
July 18, 2025 at 7:08 PM
For those of you attending academic conferences in the U.S., after October 1, 2025 please note the following:
Reposted by Ryan Fink
(1/3) Excited to introduce our new GRAB sensors for a series of steroid hormones! These tools enable real-time detection of steroid hormone dynamics in vivo🐭🧠. Happy to share these sensors and welcome any feedback! Please contact [email protected] for information.
July 10, 2025 at 3:15 PM
(1/3) Excited to introduce our new GRAB sensors for a series of steroid hormones! These tools enable real-time detection of steroid hormone dynamics in vivo🐭🧠. Happy to share these sensors and welcome any feedback! Please contact [email protected] for information.
Reposted by Ryan Fink
A @csisingapore.bsky.social team showed D-I-Tasser can outperform AlphaFold2/3, elevating high-accuracy protein structure & function predictions. We wonder if this moves us closer to resolving protein–protein complex structure prediction? @natbiotech.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
July 1, 2025 at 2:15 PM
A @csisingapore.bsky.social team showed D-I-Tasser can outperform AlphaFold2/3, elevating high-accuracy protein structure & function predictions. We wonder if this moves us closer to resolving protein–protein complex structure prediction? @natbiotech.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ryan Fink
New plasmids available from the @massecklab.bsky.social! PinkyCaMP, a mScarlet-based calcium sensor, can be leveraged for its exceptional brightness, photostability, and multiplexing capabilities.
https://twp.ai/4iobS2
https://twp.ai/4iobS2
June 26, 2025 at 12:00 PM
New plasmids available from the @massecklab.bsky.social! PinkyCaMP, a mScarlet-based calcium sensor, can be leveraged for its exceptional brightness, photostability, and multiplexing capabilities.
https://twp.ai/4iobS2
https://twp.ai/4iobS2
Reposted by Ryan Fink
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate
Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...
www.biorxiv.org
April 19, 2025 at 6:42 AM
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...
Reposted by Ryan Fink
mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
June 18, 2025 at 12:16 AM
mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
Reposted by Ryan Fink
New powerful article up in the New York Times on cancelled and delayed grants
www.nytimes.com/interactive/...
www.nytimes.com/interactive/...
Here Are the Nearly 2,500 Medical Research Grants Canceled or Delayed by Trump (Gift Article)
Some cuts have been starkly visible, but the country’s medical grant-making machinery has also radically transformed outside the public eye.
www.nytimes.com
June 4, 2025 at 3:01 PM
New powerful article up in the New York Times on cancelled and delayed grants
www.nytimes.com/interactive/...
www.nytimes.com/interactive/...
Reposted by Ryan Fink
Happy to see my Postdoc work out @natbiotech.nature.com. Using HaloTag and synthetic fluorophores instead of FPs enables us to shift the spectral properties of kinase activity reporters (KARs) to the far-red 🔬. Huge thanks to everyone involved @jinzhanglab.bsky.social!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks - Nature Biotechnology
Far-red kinase biosensors are applied to image kinase signaling networks at super resolution.
www.nature.com
April 21, 2025 at 9:28 AM
Happy to see my Postdoc work out @natbiotech.nature.com. Using HaloTag and synthetic fluorophores instead of FPs enables us to shift the spectral properties of kinase activity reporters (KARs) to the far-red 🔬. Huge thanks to everyone involved @jinzhanglab.bsky.social!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ryan Fink
First PinkyCaMP plasmids are available via @addgene.bsky.social
www.addgene.org/browse/artic...
Cc @finkryan.bsky.social @massecklab.bsky.social
www.addgene.org/browse/artic...
Cc @finkryan.bsky.social @massecklab.bsky.social
Addgene: PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities.
www.addgene.org
February 25, 2025 at 8:12 PM
First PinkyCaMP plasmids are available via @addgene.bsky.social
www.addgene.org/browse/artic...
Cc @finkryan.bsky.social @massecklab.bsky.social
www.addgene.org/browse/artic...
Cc @finkryan.bsky.social @massecklab.bsky.social