Dominic Zoller
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Dominic Zoller
@farblosbunt.bsky.social
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PhD student at ZMBP Tübingen
Plasma membranes, sptPALM and more
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Reposted by Dominic Zoller
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🔬 EXPERT VIEW 🔬

Oven-Krockhaus et al. introduce single-particle tracking photoactivated localization microscopy for studying plasma membrane proteins in plants, outlining key applications and practical guidance on instrumentation, probes, and analysis.

🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪
Fig. 1 (shortened, full legend in paper): Workflow of single-particle tracking photoactivated localization microscopy (sptPALM) analysis. (A) Localization: single molecule spots are cut out of the raw data image stack, fitted with two-dimensional Gaussian functions and the results (most importantly, their coordinates) stored as lists. (B) Tracking: single-molecule positions are grouped to tracks, based on their relative positions in subsequent frames. (C) Mean square displacement (MSD) analysis: the average squared distance the molecules have traveled in certain time intervals (top left) can be plotted in MSD curves (bottom), showing a straight line for free Brownian motion (dark gray), for which the slope is directly proportional to the diffusion coefficient. Other motion types like superdiffusive (red) or subdiffusive (yellow) show different curve shapes (inset: corresponding single-molecule example tracks).
December 28, 2025 at 9:20 AM