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Reposted by Journal of Experimental Botany

Plant Editors @planteditors.bsky.social · 16h

Some very nice #OpenAccess #grapevine #reviews in that @jxbotany.bsky.social issue, on rootstock ideotypes, #ClimateChange, and grapevine–arbuscular mycorrhizal fungi interactions!

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Reposted by Journal of Experimental Botany

Plant Editors @planteditors.bsky.social · 16h

And their special issue from August, "Grapevine Research: Expanding Knowledge to Secure a Sustainable Future" academic.oup.com/jxb/issue/76...

Volume 76 Issue 11 | Journal of Experimental Botany | Oxford Academic

The official journal of the Society of Experimental Botany. Publishes papers that describe novel and rigorous research addressing broad principles in plant science.

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Reposted by Journal of Experimental Botany

Plant Editors @planteditors.bsky.social · 16h

And ok this is a bit older than a week, but cheers to @jxbotany.bsky.social for this paper from their special issue! bsky.app/profile/jxbo...

Journal of Experimental Botany @jxbotany.bsky.social · Aug 26

🧬 SPECIAL ISSUE RESEARCH 🍇

Metabolic QTL analysis reveals the genetic architecture underlying grape berry wax formation and identifies VvTTPS12 as a β-amyrin synthase contributing to the formation of the triterpene oleanolic acid - Vervalle et al.

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

Fig. 1.Sampling strategy utilized in this study. Overview of the strategy used to sample the grape population ‘Deckrot’×G1-7720 indicating the sampling year (Y1 or Y2), number and description of individuals, and the phenological developmental stages of sampling. Furthermore, the analyses and results for which these samples were used are provided. Berry colour segregated in the progeny: white berries are indicated in green (e.g. DG225) and black berries are indicated in purple (e.g. DG133). Image created in BioRender.com/x3015jc. DR, ‘Deckrot’; G1, G1-7720; QTL, quantitative trait locus.

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Journal of Experimental Botany @jxbotany.bsky.social · 17h

🍅🌱 SPECIAL ISSUE RESEARCH 🌱🍅

Resistance against bacterial wilt in tomato is linked to variety-specific proteomic changes; the CAPE1 peptide restricts Ralstonia solancearum growth in planta - Zhang et al. 🦠🍅

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪 @bactodeath.bsky.social

Fig. 3.PR1 protein features and alignment. (A) Representation of the tomato PR1 protein domains and conserved features. SP, signal peptide; CAP, cysteine-rich secretory proteins, antigen 5, and pathogenesis-related 1 protein domain, highlighting CAP3, CAP4, CAP1, CAP2, and CBM motifs; CAPE, CAP-derived peptide (Han et al., 2023). (B) Alignment of the representative PR1 proteins across different plant species. Amino acid alignment generated from ClustalO alignment of the representative PR1 proteins from tomato (Solanum lycopersicum), potato (Solanum tuberosum), pepper (Capsicum anuum), tobacco (Nicotiana tabacum), Arabidopsis (Arabidopsis thaliana), and wheat (Triticum aestivum). Red and blue highlighted regions show the last amino acid before the putative CAPE peptide cleavage. The conserved CAPE peptide sequence is shown in red.

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Journal of Experimental Botany @jxbotany.bsky.social · 17h

🌱 SPECIAL ISSUE RESEARCH 🌱

Thylakoid protein EGY1 interacts with the magnesium chelatase H subunit to regulate chlorophyll accumulation, balance chlorophyll synthesis and protein homeostasis, and influence chloroplast development in var2 - Zhang et al.

🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪

Fig. 6 (shortened, full legend in paper): Mutation of cpSRP54/PGA4 alleviates the chloroplast development defect in var2-4 evr4-1. (A) Representative 2-week-old wild type (WT), var2-4, evr4-1, pga4-1, var2-4 pga4-1, evr4-1 pga4-1, var2-4 evr4-1, and var2-4 evr4-1 pga4-1 plants. Scale bar: 1.0 cm. (B) Accumulation of photosynthetic proteins (D1, LhcB2, PsaD, Cytf, AtpA, and RbcL) in the rosette leaves from (A). Protein loading was normalized to equal fresh tissue weight and confirmed by CBB-stained polyvinylidene difluoride membranes. The experiments in (B) were repeated independently twice with similar results. (C) The observation of chlorophyll fluorescence in the rosette leaves from (A). ms, milliseconds. The exposure time was labeled in order to compare apparent fluorescence signal intensities from different genotypes. Fluorescence is merged with the differential interference contrast image. Scale bar: 10.0 μm.

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Journal of Experimental Botany @jxbotany.bsky.social · 1d

🌱 📖 SPECIAL ISSUE REVIEW 📖 🌱

Qi et al. address current challenges in revealing protease roles in biological processes and present systematic methodologies for identifying bona fide protease–substrate pairs in plants 🔬

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪 @simonstael.bsky.social

Fig. 1.Guidelines for a bona fide protease–substrate pair.

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Journal of Experimental Botany @jxbotany.bsky.social · 1d

🌱 SPECIAL ISSUE RESEARCH 🌱

26S proteasome disassembly occurs during leaf senescence. While proteasomal subunit genes are up-regulated, proteasome protein levels do not increase. However, cytokinin application enhances proteasome activity - Wang et al.

🔗 doi.org/10.1093/jxb/...
#PlantScience 🧪

Fig. 3 (shortened, full legend in paper): Regulation of proteasomal subunits during proteotoxic stress and leaf senescence. (A) Transcriptional response to proteotoxic stress of genes coding for proteasome subunits as assayed by promoter–GUS lines. Seedlings grown for 10 d on half-strength Murashige and Skoog (MS) medium with or without 15μM MG132 were stained for GUS activity and imaged. (B) Proteasomal subunit abundance during proteotoxic stress. Total protein extracts from 10-day-old wild-type seedlings treated for 2 d with either 30 μM MG132, 2 μM bortezomib, or DMSO were assessed by western blotting using protein-specific antibodies. Coomassie Brilliant Blue- (CBB) stained gels were used as loading control. (C) Expression of proteasomal subunit genes during leaf senescence as visualized by promoter–GUS reporter lines. Shown are the results for the first leaf pair, harvested from either 15-day-old plants (young) or 43-day-old plants (old).

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Journal of Experimental Botany @jxbotany.bsky.social · 2d

🌱 SPECIAL ISSUE RESEARCH 🌱

🔬 ECLIPSE is a potential adaptor protein that links ubiquitinated inner nuclear membrane proteins to the CDC48 complex, facilitating their degradation and maintaining nuclear membrane integrity in plants - Calvanese et al.

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

Fig. 1 (shortened, full legend in paper): ECLIPSE is closely associated with plant INMAD components. (A) Schematic diagram illustrating the identification of potential INMAD components using proximity labeling with SUN1 and PUX5 as baits. SUN1, a substrate of the INMAD pathway, undergoes polyubiquitination by unidentified E3 ligases. The ubiquitinated SUN1 is recognized by a potential adaptor protein, which facilitates the recruitment of CDC48 for subsequent retrotranslocation and degradation of SUN1. PUX5 is a previously reported negative regulator of the INMAD pathway, which interacts with CDC48 and functions to prevent SUN1 degradation. (B) Reanalysis of previously published MS data obtained from proximity labeling proteomics using HA-BioID2-SUN1 and PUX5-BioID2-HA transgenic plants. Specific proteins probed by each bait were identified using biotin mock-treated transgenic plants as controls.

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Reposted by Journal of Experimental Botany

Journal of Experimental Botany @jxbotany.bsky.social · 2d

🌱 SPECIAL ISSUE REVIEW 🌱

Jeran et al discuss the trafficking of plastid-targeted proteins, focusing on regulatory bottlenecks and mislocalization. PSBO, a PSII subunit, may link proplastid-to-chloroplast differentiation with plastid quality control 🔬

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

Fig. 3.Chloroplast quality control and degradation pathways. Accumulation of damaged proteins within chloroplasts triggers the nuclear expression of cpUPR-related genes, encoding plastid-targeted chaperones and proteases, in an attempt to restore chloroplast proteostasis. However, the prolonged presence of faulty proteins and aggregates, exacerbated by ROS and other stressors, activates chloroplast-dismantling mechanisms. These include whole-chloroplast autophagosome-mediated degradation (chlorophagy), chloroplast vesiculation-mediated pathways (CV), or fission-type microautophagy, similar to those observed in mitochondria. Ubiquitin (light blue circles, Ub) and ATG8-dependent pathways (ATG8 purple circles; autophagosome depicted in blue) play crucial roles in marking chloroplasts for degradation. Ultimately, the accumulation of damaged chloroplasts within the cell leads to vacuole-mediated programmed cell death.

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Reposted by Journal of Experimental Botany

Ryo Yokoyama @yokoyama-ryo.bsky.social · 2d

13CO2 labelling: new uses for a well-established photosynthesis research tool academic.oup.com/jxb/article/... @jxbotany.bsky.social

Validate User

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Journal of Experimental Botany @jxbotany.bsky.social · 2d

🌱 SPECIAL ISSUE REVIEW 🌱

Jeran et al discuss the trafficking of plastid-targeted proteins, focusing on regulatory bottlenecks and mislocalization. PSBO, a PSII subunit, may link proplastid-to-chloroplast differentiation with plastid quality control 🔬

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

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Journal of Experimental Botany @jxbotany.bsky.social · 2d

🌱 📖 SPECIAL ISSUE REVIEW 📖 🌱

🔬 Mantz et al. review current methods and developments for mass spectrometry-based identification of protein termini and discuss their use for plant protease substrate identification 🔬

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪 @degradomics.bsky.social

Fig. 2.Plant proteases involved in various stages of plant life cycle, organelle import, and responses to abiotic or biotic stress. Important processes and pathways regulated by proteases mentioned in the text are indicated, and proteases investigated by N-terminome approaches are named. Green cells represent healthy cells, while (dark) brown cells represent dying/dead cells. The apoplast is displayed in grey color. Nitrogen-fixing bacteria are schematically depicted in red colour, the cyanobacterium Synechocystis is indicated in the puddle next to the plant, and the green alga C. reinhardtii in the soil. Blue and violet ‘Pac-Mans’ depict plant and pathogen proteases, respectively. Created in BioRender. Huesgen Lab (2025) https://BioRender.com/6aue2gv.

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Journal of Experimental Botany @jxbotany.bsky.social · 3d

📝 SPECIAL ISSUE REVIEW 📝

Hoernstein et al. consolidate information on Acylamino acid-releasing enzyme, a serine protease affecting plant development & aging, & emphasize its conserved features across all kingdoms of life 🌱🦠🧫

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪 @reskilab.bsky.social

Fig. 3 (shortened, full legend in paper): Summary of the current knowledge of acylamino acid-releasing enzyme (AARE) localization and function in plants and animals. AtAARE (green) is depicted as plant AARE and human AARE (blue) as animal AARE. Structures of both isoforms (AF-Q84LM4-F1, AF-P13798-F1; https://alphafold.ebi.ac.uk/) were predicted with AlphaFold (Jumper et al., 2021; Varadi et al., 2024). Middle shows commonalities between plants and animals: Localization of AARE to the nucleus and to the cytosol has been shown in Arabidopsis and human cell lines (Shimizu et al., 2003; Nakai et al., 2012; Zeng et al., 2017; Hoernstein et al., 2023). AARE functions as part of the antioxidant defence system in plants and animals (Shimizu et al., 2003; Nakai et al., 2012; Gogliettino et al., 2014; Riccio et al., 2015; Zeng et al., 2017). Left (only plants): AARE localizes to both plastids and mitochondria in plants (Hoernstein et al., 2023).

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Reposted by Journal of Experimental Botany

SEBiology @sebiology.bsky.social · 3d

🎥 @jxbotany.bsky.social 75th Anniversary Symposium recordings are now available and exclusively free for SEB members!

Watch sessions on Beginnings, Growth, Maturity & Future 🌱

Watch them now:
www.sebiology.org/resource/all...

All Recordings from the JXB 75th Anniversary Symposium are now online

Access every recording from the Journal of Experimental Botany 75th Anniversary Symposium! Available on demand exclusively for SEB members.

www.sebiology.org

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Journal of Experimental Botany @jxbotany.bsky.social · 3d

🔒🌿 SPECIAL ISSUE REVIEW 🔒🌿

Paiva-Silva et al. summarize and integrate current knowledge on protease inhibition in plant–pathogen interactions and speculate on ways in which this could be harnessed to improve plant resilience to biotic stresses 🦠💪

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

Fig. 1.Events of protease inhibition in plant biotic stress at three different levels. (A) Inhibition of endogenous plant proteases by plant protease inhibitors; (B) inhibition of pathogen/pest proteases by plant-derived protease inhibitors; (C) inhibition of plant proteases by pathogen-derived protease inhibitors. All proteases are denoted by the name of their homologue in Arabidopsis unless indicated otherwise. See paper for figure legend with abbreviations explanation.

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Reposted by Journal of Experimental Botany

Journal of Experimental Botany @jxbotany.bsky.social · 4d

⚡ SPECIAL ISSUE REVIEW⚡

🌱 In this review, Yu & Feng establish a phylogenetically-guided classification framework of plant aspartic proteases, clarify their roles in development, stress, and adaptation, and identify future research priorities 🌱

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

Fig. 5.Roles of plant aspartic proteases during gametogenesis and fertilization. (A) Diagram of an Arabidopsis flower; (B) PCS1 and UNDEAD mediated tapetum PCD; (C) OsAP25 and OsAP37 mediated PCD in rice; (D) A36, A39, and OsAP65 mediated pollen germination and pollen tube growth; (E) diagram of Arabidopsis mature embryo sac, showing aspartic proteases expressed in egg cells and central cells; (F) roles of ECS1/2 during fertilization.

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Reposted by Journal of Experimental Botany

Ryo Yokoyama @yokoyama-ryo.bsky.social · 4d

Chloroplast-Localized ABC Transporter OsABCB24 Regulates Aleurone Cell Size and Grain Nutritional Quality in Rice by Modulating Auxin Homeostasis academic.oup.com/jxb/article-... @jxbotany.bsky.social

The Chloroplast-Localized ABC Transporter OsABCB24 Regulates Aleurone Cell Size and Grain Nutritional Quality in Rice by Modulating Auxin Homeostasis

Abstract. The aleurone in cereal grains is an outer cell layer enriched with multiple nutrients important for human health. Enhancing the thickness of the

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Reposted by Journal of Experimental Botany

Ryo Yokoyama @yokoyama-ryo.bsky.social · 4d

Defects in ethanol fermentation catalysed by pyruvate decarboxylases reduce arsenic levels in rice academic.oup.com/jxb/article-... @jxbotany.bsky.social

Defects in ethanol fermentation catalysed by pyruvate decarboxylases reduce arsenic levels in rice

Abstract. Reducing arsenic levels in paddy rice is an important agricultural issue. Rice alcohol dehydrogenase 2 (ADH2) deficiency results in reduced arsen

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Journal of Experimental Botany @jxbotany.bsky.social · 4d

⚡ SPECIAL ISSUE REVIEW⚡

🌱 In this review, Yu & Feng establish a phylogenetically-guided classification framework of plant aspartic proteases, clarify their roles in development, stress, and adaptation, and identify future research priorities 🌱

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

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Reposted by Journal of Experimental Botany

Ralf Reski @reskilab.bsky.social · 4d

Interesting review in JXB:
How and why plants came to smell green: the origins, biosynthesis, and roles of green leaf volatiles
#plantscience
academic.oup.com/jxb/advance-...

How and why plants came to smell green: the origins, biosynthesis, and roles of green leaf volatiles

Rapidly released from damaged cells, green leaf volatiles exemplify an evolutionary adaptation conserved across terrestrial plants, serving as essential de

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Reposted by Journal of Experimental Botany

Plant Editors @planteditors.bsky.social · 5d

Very cool #PlantScience in @jxbotany.bsky.social !

Thomas WIDIEZ @thomaswidiez.bsky.social · 6d

🌽 Maize vs. Fusarium 🌽

Overexpressing a maize lipoxygenase (ZmLOX4) boosts resistance to Fusarium infection.

Glad that our maize Gene editing & transformation platform, at @rdplab.bsky.social, contributed to this research, led by the Lanubile's Lab.

▶️ doi.org/10.1093/jxb/...

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Reposted by Journal of Experimental Botany

Thomas WIDIEZ @thomaswidiez.bsky.social · 6d

🌽 Maize vs. Fusarium 🌽

Overexpressing a maize lipoxygenase (ZmLOX4) boosts resistance to Fusarium infection.

Glad that our maize Gene editing & transformation platform, at @rdplab.bsky.social, contributed to this research, led by the Lanubile's Lab.

▶️ doi.org/10.1093/jxb/...

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Journal of Experimental Botany @jxbotany.bsky.social · 5d

📖 SPECIAL ISSUE REVIEW 📖

In this review, Wang et al. describe the protease, chaperone-like, and substrate processing working modes of plant FtsH, and summarize the role of FtsH in organelle protein homeostasis 🔄 🌱

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

Box 1. Key developments in understanding FtsH functions for organelle protein homeostasis in Arabidopsis The schematic illustration shows the primary and 3D structure of FtsH (A–C) and highlights recent advances in understanding the roles of plant FtsHs localized in chloroplasts (D, E, and H) and mitochondria (F and G).
See paper for full description of Box 1.

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Journal of Experimental Botany @jxbotany.bsky.social · 6d

📖 SPECIAL ISSUE REVIEW 📖

🛡️ RD21-like proteases act in immunity and are targeted by unrelated effectors produced by a diverse range of plant pathogens throughout the plant kingdom - Huang & van der Hoorn 📝

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪

Fig. 3.AlphaFold Multimer (AFM)-predicted models of RD21-like proteases and four different inhibitors. RD21 and its orthologues are shown in a pale green surface representation with the active site (red) and inhibitors are shown in light blue as cartoon and lines with disulfides and interface residues shown as sticks. AFM scores and PDB files of these models are available in Supplementary Table S1 and Supplementary Dataset S1, respectively.

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Journal of Experimental Botany @jxbotany.bsky.social · 6d

💡 SPECIAL ISSUE VIEWPOINT 💡

Immune proteases are promising targets for protein engineering 🛠️ to boost disease resistance in plants 🌾 - Schuster et al.

🔗 doi.org/10.1093/jxb/...

#PlantScience 🧪 @marischuster.bsky.social @aciattoni.bsky.social

Fig. 1.Four classes of roles of immune proteases illustrated via examples. (i) Pathogen perception: Required for Cladosporium Resistance-3 (Rcr3) protease is inhibited by the fungal avirulence effector Avr2. The Rcr3-Avr2 complex is recognized by the immune receptor Cf-2, triggering a defence response (Kruger et al, 2002). (ii) Regulation of the immune response: METACASPASE 4 (MC4) is activated by calcium upon wounding or pathogen attack. MC4 cleaves tonoplast-located ProPEP1 releasing PEP1 to the apoplast where it is perceived by PEP RECEPTORS (PEPRs) thereby initiating defence responses (Hander et al, 2019). (iii) Counteracting pathogen effectors: soybean aspartic protease GmAP5, degrades the Phytophthora sojae virulence factor glycoside hydrolase family 12 (GH12) protein, XEG1 (Xia et al., 2020). (iv) Direct pathogen attack: secreted aspartic proteases (SAPs) cleave Pseudomonas syringae MucD protein thereby suppressing bacterial growth (Wang et al., 2019).

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