Fabrice Jean-Pierre
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Fabrice Jean-Pierre
@fabricejpierre.bsky.social
3.7K followers 520 following 180 posts
Assistant Professor @USherbrooke | Polymicrobial biofilms, interactions & mechanisms of recalcitrance | he/him

https://www.fjplab.com/
https://scholar.google.ca/citations?user=N5UlGZgAAAAJ&hl=en
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Reposted by Fabrice Jean-Pierre
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Gac Is a Transcriptional Repressor of the Lyme Disease Spirochete's OspC Virulence-Associated Surface Protein

Castro-Padovani, Saylor, Husted, Krusenstjerna, Jusufovic, and Stevenson
2023, J Bacteriol, 205:e0044022.
doi: 10.1128/jb.00440-22
pubmed.ncbi.nlm.nih.gov/36920207/
#MicroSky
(A) Electrophoretic mobility shift assay (EMSA) of labeled Ip89 ospC operator, without (−) and with (+) cytoplasmic extract from strain B31. The asterisk indicates shifted probe that was present only when borrelial extract was included, indicating presence of a DNA-binding component. Unbound DNA is indicated by “free.” Some labeled DNA probe remained in the wells of the gel. (B) Cytoplasmic extract from strain B31 was incubated with Ip89 ospC operator DNA affixed to beads, eluted with increasing concentrations of NaCl, and eluates were separated by SDS-polyacrylamide gel electrophoresis. Lanes are labeled with the concentration of NaCl used for that elution. The arrow indicated a protein band of 30 to 35 kDa that eluted in the 0.3 and 0.5 mM NaCl washes. (C) Amino acid sequence of GyrA and Gac (the two potential initiation methionines of Gac are indicated by boldface and underlining). Mass spectrometry identified nine unique peptides that all mapped to the Gac ORF. Peptides identified with high confidence are shaded green, and those with lower confidence are red. (D) Predicted structures of B. burgdorferi Gac and GyrA proteins, as determined by AlphaFold (https://alphafold.ebi.ac.uk). Both are consistent with the solved structures of B. burgdorferi Gac and the N-terminal domain of E. coli GyrA (https://www.rcsb.org/structure/4ckk).
November 7, 2025 at 3:51 PM
fabricejpierre.bsky.social
Finally! Prevotella is getting the love it deserves :-)
November 7, 2025 at 12:43 AM