You will identify immune mechanisms underlying neuroinflammatory autoimmune diseases in children vs adults--autoimmune encephalitis (AE) and multiple sclerosis (MS). You will apply multi-omics single-cell assays to study the role of B/plasma cells and antibodies using patient samples (CSF and blood)

Main dish or Appetizer
1. Adas Polo & Lamb (Fatemeh Yousefian and Masoud Ilkhani)
2. Lasagna Bolognese (Alex Nazzari & Matt Stern)

Dessert
1. Mohanthal (Hardy Nariya)
2. Cesta di Frutta @katyely.bsky.social

While #FlowLITE quantifies total antibodies, #FlowBEAT also quantifies antibody specificities to defined antigens. Both protocols can be adapted to your research needs.
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star-protocols.cell.com/protocols/4572

For context: FlowLITE was based on our previous technology, FlowBEAT, which we developed to measure human antibody isotypes against #SARSCoV2 and autoantigens in the #nasal #mucosa of #COVID19 patients (Sci Transl Med, 2024)
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www.science.org/doi/10.1126/...

#FlowLITE is a modular, easy-to-build, bead-based assay for characterizing & quantifying absolute number of antibodies in small volumes (~10μL) of any fluid specimen. This is Justin Hahm’s Honors Thesis project (Highest Honors!). He was mentored by PhD student Alex Nazzari, the co-first author.
2/4

Main dish or Appetizer
1. Adas Polo & Lamb (Fatemeh Yousefian and Masoud Ilkhani)
2. Lasagna Bolognese (Alex Nazzari & Matt Stern)

Dessert
1. Mohanthal (Hardy Nariya)
2. Cesta di Frutta @katyely.bsky.social