David Negus
@davidnegus.bsky.social
Microbiologist, lover of phage and predatory bacteria. Senior lecturer at Nottingham Trent University. Department of prokaryotes culturable.
Reposted by David Negus
🚨Preprint alert - this is a big one! We transfer the revolutionary power of TnSeq to bacteriophages.
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
November 20, 2025 at 8:40 PM
🚨Preprint alert - this is a big one! We transfer the revolutionary power of TnSeq to bacteriophages.
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Our HIDEN-SEQ links the "dark matter" genes of your favorite phage to any selectable phenotype, guiding the path from fun observations to molecular mechanisms.
A thread 1/8
Reposted by David Negus
Delighted for @mohamedeladawy.bsky.social on the publication of the "big one" from his PhD work.
- Largest genome-based study on uropathogens in Egypt.
- AMR genes for Pseudomonas, and those associated with meropenem poorly understood.
- Prediction databases need better curation.
#UTIsky #microsky
- Largest genome-based study on uropathogens in Egypt.
- AMR genes for Pseudomonas, and those associated with meropenem poorly understood.
- Prediction databases need better curation.
#UTIsky #microsky
Phenotype–genotype discordance in antimicrobial resistance profiles of Gram-negative uropathogens recovered from catheter-associated urinary tract infections in Egypt
AbstractObjectives. Catheter-associated urinary tract infections (CAUTIs) are among the most common healthcare-associated infections in low- and middle-inc
academic.oup.com
September 23, 2025 at 5:18 PM
Delighted for @mohamedeladawy.bsky.social on the publication of the "big one" from his PhD work.
- Largest genome-based study on uropathogens in Egypt.
- AMR genes for Pseudomonas, and those associated with meropenem poorly understood.
- Prediction databases need better curation.
#UTIsky #microsky
- Largest genome-based study on uropathogens in Egypt.
- AMR genes for Pseudomonas, and those associated with meropenem poorly understood.
- Prediction databases need better curation.
#UTIsky #microsky
Reposted by David Negus
If you are UK-based and working on any aspect of microbiomes (human, plant, insect, soil, animal, ...), please do sign up to Microbiome-Net for details of networking, funding and training opportunities.
forms.office.com/pages/respon...
forms.office.com/pages/respon...
September 13, 2025 at 6:31 AM
If you are UK-based and working on any aspect of microbiomes (human, plant, insect, soil, animal, ...), please do sign up to Microbiome-Net for details of networking, funding and training opportunities.
forms.office.com/pages/respon...
forms.office.com/pages/respon...
Wow, 17,000 applications!
marie-sklodowska-curie-actions.ec.europa.eu/news/msca-po...
marie-sklodowska-curie-actions.ec.europa.eu/news/msca-po...
marie-sklodowska-curie-actions.ec.europa.eu
September 11, 2025 at 10:31 PM
Wow, 17,000 applications!
marie-sklodowska-curie-actions.ec.europa.eu/news/msca-po...
marie-sklodowska-curie-actions.ec.europa.eu/news/msca-po...
You have to love Belgium. A country built on beers, bikes and bacteriophage.
September 10, 2025 at 8:04 PM
You have to love Belgium. A country built on beers, bikes and bacteriophage.
Reposted by David Negus
We have a position for an Assist Prof in Microbial Genomics in our School of III.
Hod Joan Geoghegan will be happy to field questions if you are interested. No specific area of microbial genomics preferred so please do consider it
www.jobs.ac.uk/job/DOO184/a...
Hod Joan Geoghegan will be happy to field questions if you are interested. No specific area of microbial genomics preferred so please do consider it
www.jobs.ac.uk/job/DOO184/a...
Assistant Professor (Research and Education) in Bacterial Genomics at University of Birmingham
Apply now for the Assistant Professor (Research and Education) in Bacterial Genomics role on jobs.ac.uk - the leading job board for higher education jobs. View details.
www.jobs.ac.uk
September 3, 2025 at 4:02 PM
We have a position for an Assist Prof in Microbial Genomics in our School of III.
Hod Joan Geoghegan will be happy to field questions if you are interested. No specific area of microbial genomics preferred so please do consider it
www.jobs.ac.uk/job/DOO184/a...
Hod Joan Geoghegan will be happy to field questions if you are interested. No specific area of microbial genomics preferred so please do consider it
www.jobs.ac.uk/job/DOO184/a...
Interested in investigating the use of bacteriocins for the treatment of urinary tract infections? Come work with me and @bugsinyourguts.bsky.social Professor Lesley Hoyles as a research associate on an exciting project funded by The Urology Foundation
vacancies.ntu.ac.uk/Job/JobDetai...
vacancies.ntu.ac.uk/Job/JobDetai...
Research Associate:Clifton Campus
vacancies.ntu.ac.uk
August 5, 2025 at 9:40 AM
Interested in investigating the use of bacteriocins for the treatment of urinary tract infections? Come work with me and @bugsinyourguts.bsky.social Professor Lesley Hoyles as a research associate on an exciting project funded by The Urology Foundation
vacancies.ntu.ac.uk/Job/JobDetai...
vacancies.ntu.ac.uk/Job/JobDetai...
Reposted by David Negus
I'm super happy to share this preprint on ‘Distribution of capsule and O types in #Klebsiella pneumoniae causing neonatal sepsis in Africa and South Asia: meta-analysis of genome-predicted serotype prevalence and potential vaccine coverage’
doi.org/10.1101/2025...
doi.org/10.1101/2025...
Distribution of capsule and O types in Klebsiella pneumoniae causing neonatal sepsis in Africa and South Asia: meta-analysis of genome-predicted serotype prevalence and potential vaccine coverage
Background: Klebsiella pneumoniae causes ~20% of sepsis in neonates, with ~40% crude mortality. A vaccine administered to pregnant women, protecting against 70% of K. pneumoniae infections, could aver...
doi.org
July 1, 2025 at 9:45 AM
I'm super happy to share this preprint on ‘Distribution of capsule and O types in #Klebsiella pneumoniae causing neonatal sepsis in Africa and South Asia: meta-analysis of genome-predicted serotype prevalence and potential vaccine coverage’
doi.org/10.1101/2025...
doi.org/10.1101/2025...
Reposted by David Negus
Great to see the typeset version of this invited review out.
Covers pretty much everything we know about metabolites/peptides produced by Klebsiella spp. and how they interact with the host. #microsky #microbiomesky @microbiotaandhost.bsky.social
doi.org/10.1530/MAH-...
Covers pretty much everything we know about metabolites/peptides produced by Klebsiella spp. and how they interact with the host. #microsky #microbiomesky @microbiotaandhost.bsky.social
doi.org/10.1530/MAH-...
Host interactions of bioactive molecules produced by Klebsiella spp
Abstract Klebsiella spp. are early colonisers and commensals of human skin, oral, nasal, throat and gut microbiotas, but are also opportunistic pathogens. Much of our information on these bacteria is ...
doi.org
May 13, 2025 at 7:56 AM
Great to see the typeset version of this invited review out.
Covers pretty much everything we know about metabolites/peptides produced by Klebsiella spp. and how they interact with the host. #microsky #microbiomesky @microbiotaandhost.bsky.social
doi.org/10.1530/MAH-...
Covers pretty much everything we know about metabolites/peptides produced by Klebsiella spp. and how they interact with the host. #microsky #microbiomesky @microbiotaandhost.bsky.social
doi.org/10.1530/MAH-...
🚨New publication🚨
We show that K. Pneumoniae bacteriophages can be incorporated into alginate to create an antibiofilm matrix effective against clinical K. pneumoniae strains
academic.oup.com/jambio/artic...
We show that K. Pneumoniae bacteriophages can be incorporated into alginate to create an antibiofilm matrix effective against clinical K. pneumoniae strains
academic.oup.com/jambio/artic...
Bacteriophage-embedded and coated alginate layers inhibit biofilm formation by clinical strains of Klebsiella pneumoniae
AbstractAims. This study aimed to determine the antibiofilm properties of Klebsiella pneumoniae phages previously isolated from Thai hospital sewage water.
academic.oup.com
April 25, 2025 at 10:47 AM
🚨New publication🚨
We show that K. Pneumoniae bacteriophages can be incorporated into alginate to create an antibiofilm matrix effective against clinical K. pneumoniae strains
academic.oup.com/jambio/artic...
We show that K. Pneumoniae bacteriophages can be incorporated into alginate to create an antibiofilm matrix effective against clinical K. pneumoniae strains
academic.oup.com/jambio/artic...
Reposted by David Negus
Very pleased to see this review out on host interactions of bioactive molecules produced by Klebsiella spp. #MicroSky
mah.bioscientifica.com
April 24, 2025 at 7:38 PM
Very pleased to see this review out on host interactions of bioactive molecules produced by Klebsiella spp. #MicroSky
Reposted by David Negus
New preprint from @mohamedeladawy.bsky.social
This represents the main body of work from Mohamed's thesis. He's carried out the largest study, to date, on G-ve (11 different genera) CAUTI-associated bacteria in Egypt. #UTIsky #Microsky #AMR
It's been a pleasure to supervise Mohamed's PhD studies.
This represents the main body of work from Mohamed's thesis. He's carried out the largest study, to date, on G-ve (11 different genera) CAUTI-associated bacteria in Egypt. #UTIsky #Microsky #AMR
It's been a pleasure to supervise Mohamed's PhD studies.
Phenotype-genotype discordance in antimicrobial resistance profiles of Gram-negative uropathogens recovered from catheter-associated urinary tract infections in Egypt https://www.biorxiv.org/content/10.1101/2025.04.17.649370v1
April 22, 2025 at 10:29 AM
New preprint from @mohamedeladawy.bsky.social
This represents the main body of work from Mohamed's thesis. He's carried out the largest study, to date, on G-ve (11 different genera) CAUTI-associated bacteria in Egypt. #UTIsky #Microsky #AMR
It's been a pleasure to supervise Mohamed's PhD studies.
This represents the main body of work from Mohamed's thesis. He's carried out the largest study, to date, on G-ve (11 different genera) CAUTI-associated bacteria in Egypt. #UTIsky #Microsky #AMR
It's been a pleasure to supervise Mohamed's PhD studies.
Reposted by David Negus
New paper from me and @davidnegus.bsky.social. We've trebled the number of authenticated webervirus genomes, and show that low-quality virome MAGs are to be avoided in comparative analyses.
academic.oup.com/femsec/advan...
academic.oup.com/femsec/advan...
Weberviruses are gut-associated phages that infect Klebsiella spp.
Abstract. Weberviruses are bacteriophages (phages) that can infect and lyse clinically relevant, multidrug-resistant (MDR) strains of Klebsiella. They are
academic.oup.com
April 19, 2025 at 8:31 AM
New paper from me and @davidnegus.bsky.social. We've trebled the number of authenticated webervirus genomes, and show that low-quality virome MAGs are to be avoided in comparative analyses.
academic.oup.com/femsec/advan...
academic.oup.com/femsec/advan...
Reposted by David Negus
Two ERC-funded postdoc positions available in the team - one here and second to follow - please repost! jobs.ncl.ac.uk/job/Newcastl...
Research Associate Genetic and functional analysis of Gram positive secretion systems and substrates
Research Associate Genetic and functional analysis of Gram positive secretion systems and substrates
jobs.ncl.ac.uk
April 9, 2025 at 12:29 PM
Two ERC-funded postdoc positions available in the team - one here and second to follow - please repost! jobs.ncl.ac.uk/job/Newcastl...
Reposted by David Negus
Congratulations to Caitlin Wildsmith on the second paper from her PhD with @davidnegus.bsky.social! #microsky
Genomic and phenotypic characterization of staphylococci isolated from the skin of non-human primates
The growth of wildlife tourism coupled with continued deforestation has resulted in increased contact between non-human primates (NHPs) and humans. Such events may promote the transmission of potentia...
www.microbiologyresearch.org
April 2, 2025 at 9:30 PM
Congratulations to Caitlin Wildsmith on the second paper from her PhD with @davidnegus.bsky.social! #microsky
Reposted by David Negus
We had a few weberviruses isolated and lots of K. pneumoniae strains/genomes but hadn't done anything with them. COVID lockdown meant Sam needed to change the focus of his PhD for a while. This is the result.
Nice collaboration with @davidnegus.bsky.social and @halllab.bsky.social, among others.
Nice collaboration with @davidnegus.bsky.social and @halllab.bsky.social, among others.
Weberviruses are gut-associated phages that infect Klebsiella spp. https://www.biorxiv.org/content/10.1101/2024.11.04.620279v1
Weberviruses are gut-associated phages that infect Klebsiella spp. https://www.biorxiv.org/content/10.1101/2024.11.04.620279v1
Weberviruses are bacteriophages (phages) that have been shown to infect and lyse members of the genu
www.biorxiv.org
November 5, 2024 at 8:16 AM
We had a few weberviruses isolated and lots of K. pneumoniae strains/genomes but hadn't done anything with them. COVID lockdown meant Sam needed to change the focus of his PhD for a while. This is the result.
Nice collaboration with @davidnegus.bsky.social and @halllab.bsky.social, among others.
Nice collaboration with @davidnegus.bsky.social and @halllab.bsky.social, among others.
Reposted by David Negus
New paper alert! New paper alert! Insight, including genomic analyses, in to Staphylococcal diversity on NHP skin. @microbiologysociety.org @davidnegus.bsky.social www.microbiologyresearch.org/content/jour...
Genomic and phenotypic characterization of staphylococci isolated from the skin of non-human primates
The growth of wildlife tourism coupled with continued deforestation has resulted in increased contact between non-human primates (NHPs) and humans. Such events may promote the transmission of potentia...
www.microbiologyresearch.org
March 25, 2025 at 5:48 PM
New paper alert! New paper alert! Insight, including genomic analyses, in to Staphylococcal diversity on NHP skin. @microbiologysociety.org @davidnegus.bsky.social www.microbiologyresearch.org/content/jour...