Or a secret email address

Tight collaboration between
@annanordin.bsky.social 🇸🇪 and Thomas A. Kluiver from the Peng Lab at the Princess Maxima Center for Pediatric Oncology 🇳🇱 🙏

Supported by
@vetenskapsradet.bsky.social
@cancerfonden
@liu.se
@kawresearch.bsky.social
@scilifelab.se
@AdditionalVentures

I'm surprised that we should be surprised:
beyond 3D genome and open chromatin—it is the DNA Consensus sequence recognition that makes this difference!
Good old transcription factors biology.

And this feature of how Wnt/β-catenin seems to be universal - not only in hepatoblastoma 👇

The mechanistic answer is not far. It is the TCF/LEF diversity that bring β-catenin here or there!

Long story short, β-catenin has different targets in the two cell types 😱

In Hepatoblastoma two subtypes coexist, the fetal (F) more liver-like, and the embryonal (E), less dedifferentiated and more aggressive

In both there is constitutively active β-catenin
How does E cells express more classical #Wnt target genes?

You are a star!

The size that matters is that if the gametes

We are the CantùLab
liu.se/en/research/...

liu.se/en/research/...
Supported by
@kawresearch.bsky.social
@Cancerfonden
@vetenskapsradet.bsky.social
@scilifelab.se
@AdditionalVentures

It’s astonishing to see these unanticipated role of DVL proteins as chromatin-associating gene regulators. I wonder how many more proteins have unexpected second functions.
Well done on breaking the DVL paradigm!

We are the CantùLab @liu.se
liu.se/en/research/...
Superb✨collaboration w/ @remeseiro-lab.bsky.social @umeauniversitet.bsky.social

Supported by
@kawresearch.bsky.social
@Cancerfonden
@vetenskapsradet.bsky.social
@scilifelab.se
@AdditionalVentures

Why this matters?

It shows that extracellular signals (e.g. #WNT) are acute genomic remodellers

The Genome - both sequence and 3D structure - is complex but in a matter of minutes it responds to external clues

This is a beautiful adaptation towards cell plasticity.

Are the CTCF-mediated 3D interactions sites (RUWs) functionally important for #WNT activation ⚒️
Yes.
The evidence?
If individual RUWs are mutated ✂️ they halve (of ~40%) the WNT>induction of ONLY that gene!

We discovered
CTCF/Cohesin Repositioning Under Wnt (RUWs):
✅new CTCF binding sites across the genome in WNT-ON genome-wide
✅RUWs are needed for proper target gene expression
✅Yes, these include the usual suspects (e.g. AXIN2)
✅RUWs are dependent on β-catenin, which interacts with CTCF