Gene regulatory network analysis pointed to NFIB as a central hub. It interacts e.g. with MEIS2 and TCF4, and drives late-stage enhancer activation. Heterochronic transplants suggests: maturation competence is sensitive to environmental timing.
#GeneRegulation #Scenic+ #NFIB #Transplantation
(6/n)

We also saw a shift in TF activity.

Footprinting showed increased NFI binding, especially NFIB, in late-born cohorts. These neurons activate maturation modules faster — possibly guided by chromatin remodeling during neurogenesis.
#TFfootprints #TranscriptionFactors #scATACseq
(5/n)

This shift was mirrored in chromatin accessibility.

scATAC-seq revealed stage-specific enhancer activation, with late-born cells opening distinct regulatory elements — especially in distal regions. A dynamic change in the regulatory landscape.
#Chromatin #Epigenetics #scATACseq
(4/n)

Using FlashTag to label isochronic cohorts, we tracked the fate of early- and late-born GABAergic neurons. Surprisingly, later-born neurons matured faster — reaching advanced transcriptional states in hours rather than days. Differentiation stayed relatively constant; maturation didn’t.
(3/n)

During corticogenesis, progenitor competence changes over time — generating distinct excitatory neuron types.
But it turns out that in inhibitory lineages, progenitors retain more stable differentiation competence. Instead, temporal progression alters maturation dynamics.
#PatchClamp
(2/n)