www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Meta-analysis 37 scRNAseq libraries + 44 scATACseq datasets->
5 Foam cell states
ICAM1+ inflammatory
FOLR2+ phagocytic
MMP9+ matrix-remodeling
ANGPTL4+ hypoxic
COL1A2+ fibroblastic
#Biomol_MDPI 2024
www.mdpi.com/2218-273X/14...
Meta-analysis 37 scRNAseq libraries + 44 scATACseq datasets->
5 Foam cell states
ICAM1+ inflammatory
FOLR2+ phagocytic
MMP9+ matrix-remodeling
ANGPTL4+ hypoxic
COL1A2+ fibroblastic
#Biomol_MDPI 2024
www.mdpi.com/2218-273X/14...
journals.plos.org/ploscompbiol...
journals.plos.org/ploscompbiol...
I also did early single-cell work—RNA-seq and ATAC-seq.
That experience paid off when I collaborated with Tao Liu, creator of MACS2 to work on scATACseq.
Tao was my hero during my PhD!
I also did early single-cell work—RNA-seq and ATAC-seq.
That experience paid off when I collaborated with Tao Liu, creator of MACS2 to work on scATACseq.
Tao was my hero during my PhD!
MaxFuse
Integration of spatial & single-cell data across modalities with weakly linked features
Fuzzy Smoothing
Iterative cycles of coembedding, graph-smoothing & matching
tri-omic scRNAseq scATACseq CODEX integration
#NatBiotech 2023
www.nature.com/articles/s41...
MaxFuse
Integration of spatial & single-cell data across modalities with weakly linked features
Fuzzy Smoothing
Iterative cycles of coembedding, graph-smoothing & matching
tri-omic scRNAseq scATACseq CODEX integration
#NatBiotech 2023
www.nature.com/articles/s41...
8 years ago, I worked on single-cell RNA-seq & ATAC-seq at Harvard FAS Informatics. At Dana-Farber, I collaborated with Tao Liu for scATACseq, creator of MACS2—the most popular peak caller. That experience was invaluable.
8 years ago, I worked on single-cell RNA-seq & ATAC-seq at Harvard FAS Informatics. At Dana-Farber, I collaborated with Tao Liu for scATACseq, creator of MACS2—the most popular peak caller. That experience was invaluable.
#OpenAccess: buff.ly/UXLfbrp
#OpenAccess: buff.ly/UXLfbrp
🎥 Dive deeper with the video: youtu.be/XNZx5Dlp6jY?...
#Bioinformatics #10xGenomics #LIBD
🎥 Dive deeper with the video: youtu.be/XNZx5Dlp6jY?...
#Bioinformatics #10xGenomics #LIBD
Footprinting showed increased NFI binding, especially NFIB, in late-born cohorts. These neurons activate maturation modules faster — possibly guided by chromatin remodeling during neurogenesis.
#TFfootprints #TranscriptionFactors #scATACseq
(5/n)
Footprinting showed increased NFI binding, especially NFIB, in late-born cohorts. These neurons activate maturation modules faster — possibly guided by chromatin remodeling during neurogenesis.
#TFfootprints #TranscriptionFactors #scATACseq
(5/n)
scRNAseq
scATACseq
CITEseq
#SpatialTranscriptomics #Visium
Marginal Reticular Cells, not fibroblastic reticular cells, are high in PDGFRβ, #FibrillarCollagen, Proteoglycan core proteins & DIO2
#Immunity 2024
www.cell.com/immunity/ful...
scRNAseq
scATACseq
CITEseq
#SpatialTranscriptomics #Visium
Marginal Reticular Cells, not fibroblastic reticular cells, are high in PDGFRβ, #FibrillarCollagen, Proteoglycan core proteins & DIO2
#Immunity 2024
www.cell.com/immunity/ful...
Work for true or sample-paired scRNAseq + scATACseq
Bayesian framework to learn hidden TF activity & generate high-confidence TF-peak-gene circuits
vs TRIPOD FigR
#NatComputSci 2023
www.nature.com/articles/s43...
Work for true or sample-paired scRNAseq + scATACseq
Bayesian framework to learn hidden TF activity & generate high-confidence TF-peak-gene circuits
vs TRIPOD FigR
#NatComputSci 2023
www.nature.com/articles/s43...
scRNAseq + scATACseq + single-cell transcription factor perturbation (CROP-seq, lentiviral gRNA library for 20 TFs, 3 gRNAs/TF, 1 gRNA/cell in >22k cells) in
GLI3-HES1 antagonism for dorsoventral patterning in human telencephalon
#Nature 2023
www.nature.com/articles/s41...
scRNAseq + scATACseq + single-cell transcription factor perturbation (CROP-seq, lentiviral gRNA library for 20 TFs, 3 gRNAs/TF, 1 gRNA/cell in >22k cells) in
GLI3-HES1 antagonism for dorsoventral patterning in human telencephalon
#Nature 2023
www.nature.com/articles/s41...
Try ShinyCell2!😆
Compatible with data formats Seurat Signac ArchR Scanpy
vs cellxgene WebAtlas Vitessce
github.com/the-ouyang-l...
bioRxiv 2024
www.biorxiv.org/content/10.1...
Try ShinyCell2!😆
Compatible with data formats Seurat Signac ArchR Scanpy
vs cellxgene WebAtlas Vitessce
github.com/the-ouyang-l...
bioRxiv 2024
www.biorxiv.org/content/10.1...
scRNAseq scATACseq
12- vs 3-mo
#Aging
1⃣Mesenchymal Phox2B/ESRRγ/HoxB7-TGFβ2➡️ #Fibrosis (steroidogenic stroma, fibroblast, theca, but NOT pericyte)
2⃣Granulosa cell HSPA5 #ATF3 ATF4➡️#ERStress
Any #VascularAging?
#GeroScience 2025
link.springer.com/article/10.1...
scRNAseq scATACseq
12- vs 3-mo
#Aging
1⃣Mesenchymal Phox2B/ESRRγ/HoxB7-TGFβ2➡️ #Fibrosis (steroidogenic stroma, fibroblast, theca, but NOT pericyte)
2⃣Granulosa cell HSPA5 #ATF3 ATF4➡️#ERStress
Any #VascularAging?
#GeroScience 2025
link.springer.com/article/10.1...
#EESAgeing #SingleCell #Aging #ATACseq #RNAseq #Immunology #Multiomics #scRNAseq #scATACseq
#EESAgeing #SingleCell #Aging #ATACseq #RNAseq #Immunology #Multiomics #scRNAseq #scATACseq
#GABA #Neuroscience #scATACseq #scRNAseq
www.nature.com/articles/s41...
1/n
#GABA #Neuroscience #scATACseq #scRNAseq
scATAC-seq revealed stage-specific enhancer activation, with late-born cells opening distinct regulatory elements — especially in distal regions. A dynamic change in the regulatory landscape.
#Chromatin #Epigenetics #scATACseq
(4/n)
scATAC-seq revealed stage-specific enhancer activation, with late-born cells opening distinct regulatory elements — especially in distal regions. A dynamic change in the regulatory landscape.
#Chromatin #Epigenetics #scATACseq
(4/n)
📊 We used single-cell multi-omics (RNA + ATAC) + bulk ATAC on PBMCs to build a cell-by-cell map of aging.
👉 Poster 83, EMBO | EMBL Symposium
#SingleCell #Aging #EMBL #Multiomics #scRNAseq #scATACseq
📊 We used single-cell multi-omics (RNA + ATAC) + bulk ATAC on PBMCs to build a cell-by-cell map of aging.
👉 Poster 83, EMBO | EMBL Symposium
#SingleCell #Aging #EMBL #Multiomics #scRNAseq #scATACseq
Happy 2025 everyone 🎆 !
Happy 2025 everyone 🎆 !
#CellRanger pipelines. This is inspired by our work at #LIBD and resources from 10x Genomics. youtu.be/fHVLi9-sftM?...
#CellRanger pipelines. This is inspired by our work at #LIBD and resources from 10x Genomics. youtu.be/fHVLi9-sftM?...
snRNAseq scATACseq 10X Visium
13 CM subsets
NFYa marks CM4-like subsets
CM NFYa KO🐭->
Embryonic death
#CardiacNoncompaction/Hypertrabeculation
⏬Proliferation
⏬Mitochondrial respiration
#DevCell 2023
www.sciencedirect.com/science/arti...
snRNAseq scATACseq 10X Visium
13 CM subsets
NFYa marks CM4-like subsets
CM NFYa KO🐭->
Embryonic death
#CardiacNoncompaction/Hypertrabeculation
⏬Proliferation
⏬Mitochondrial respiration
#DevCell 2023
www.sciencedirect.com/science/arti...