Andrew Plested
@andrewplested.bsky.social
Glutamate
ORCID: 0000-0001-6062-0832
ORCID: 0000-0001-6062-0832
Your daily reminder that AI "knowledge" is low quality.
Does PGE2 (in inflammation) activate/upregulate sodium #ionchannels important in pain? It's unclear.
AI-answer #1 ("PGE activation of NaV") is a dud, it's wrong.
AI-answer #2 ("PGE activation of TTX-R") is bluster, and lacks perspective.
Does PGE2 (in inflammation) activate/upregulate sodium #ionchannels important in pain? It's unclear.
AI-answer #1 ("PGE activation of NaV") is a dud, it's wrong.
AI-answer #2 ("PGE activation of TTX-R") is bluster, and lacks perspective.
November 12, 2025 at 5:54 AM
Your daily reminder that AI "knowledge" is low quality.
Does PGE2 (in inflammation) activate/upregulate sodium #ionchannels important in pain? It's unclear.
AI-answer #1 ("PGE activation of NaV") is a dud, it's wrong.
AI-answer #2 ("PGE activation of TTX-R") is bluster, and lacks perspective.
Does PGE2 (in inflammation) activate/upregulate sodium #ionchannels important in pain? It's unclear.
AI-answer #1 ("PGE activation of NaV") is a dud, it's wrong.
AI-answer #2 ("PGE activation of TTX-R") is bluster, and lacks perspective.
Moon just before it rolled down this mountain into the Tagliamento valley.
November 10, 2025 at 8:43 AM
Moon just before it rolled down this mountain into the Tagliamento valley.
Calcium and magnesium in the pore of NMDA receptors!
Lipids play an unexpected role. The hydration shells of Ca2+ and Mg2+ could be the difference between permeation and block. So much physiology in the energetics of H2O.
Very stimulating work! Congrats @lab-furukawa.bsky.social
#iglurs
Lipids play an unexpected role. The hydration shells of Ca2+ and Mg2+ could be the difference between permeation and block. So much physiology in the energetics of H2O.
Very stimulating work! Congrats @lab-furukawa.bsky.social
#iglurs
November 8, 2025 at 6:30 AM
Calcium and magnesium in the pore of NMDA receptors!
Lipids play an unexpected role. The hydration shells of Ca2+ and Mg2+ could be the difference between permeation and block. So much physiology in the energetics of H2O.
Very stimulating work! Congrats @lab-furukawa.bsky.social
#iglurs
Lipids play an unexpected role. The hydration shells of Ca2+ and Mg2+ could be the difference between permeation and block. So much physiology in the energetics of H2O.
Very stimulating work! Congrats @lab-furukawa.bsky.social
#iglurs
My name is Kevin Rowland, and I’m the leader of the band…
October 18, 2025 at 7:52 AM
My name is Kevin Rowland, and I’m the leader of the band…
Ingenious concept - light sheet on serial sections by photo-etching the expanded hydrogel, layer by layer.
Photo-sectioning is quite slow, and the volumetric images are insanely large and complex, but the data look wonderful.
www.science.org/doi/10.1126/...
Photo-sectioning is quite slow, and the volumetric images are insanely large and complex, but the data look wonderful.
www.science.org/doi/10.1126/...
October 17, 2025 at 7:00 AM
Ingenious concept - light sheet on serial sections by photo-etching the expanded hydrogel, layer by layer.
Photo-sectioning is quite slow, and the volumetric images are insanely large and complex, but the data look wonderful.
www.science.org/doi/10.1126/...
Photo-sectioning is quite slow, and the volumetric images are insanely large and complex, but the data look wonderful.
www.science.org/doi/10.1126/...
Perhaps even more incredible are the full field of view synchronised events, measured with our high-affinity sensor iGlu3-Slow2. (No we don't know what they are, thanks for asking). We also see them (less often) with iGluSnFR3, and basically never with iGlu3Fast (once after damage to slice).
4/6
4/6
October 12, 2025 at 4:24 PM
Perhaps even more incredible are the full field of view synchronised events, measured with our high-affinity sensor iGlu3-Slow2. (No we don't know what they are, thanks for asking). We also see them (less often) with iGluSnFR3, and basically never with iGlu3Fast (once after damage to slice).
4/6
4/6
Katalin made some mutants of iGluSnFR3 that have improved properties. They end up being similar to (although not the same as) the new iGluSnFR4 variants.
iGlu3Fast is quite incredible in 2-Photon. This is a hippocampal dendrite, spontaneous activity. A world away from 1 Hz fluctuations!
2/6
iGlu3Fast is quite incredible in 2-Photon. This is a hippocampal dendrite, spontaneous activity. A world away from 1 Hz fluctuations!
2/6
October 12, 2025 at 4:24 PM
Katalin made some mutants of iGluSnFR3 that have improved properties. They end up being similar to (although not the same as) the new iGluSnFR4 variants.
iGlu3Fast is quite incredible in 2-Photon. This is a hippocampal dendrite, spontaneous activity. A world away from 1 Hz fluctuations!
2/6
iGlu3Fast is quite incredible in 2-Photon. This is a hippocampal dendrite, spontaneous activity. A world away from 1 Hz fluctuations!
2/6
Walking to the station in Dresden. Some architecture from across the eras.
September 30, 2025 at 12:29 PM
Walking to the station in Dresden. Some architecture from across the eras.
Hotel carpet, yes carpet, is a trip.
September 30, 2025 at 8:45 AM
Hotel carpet, yes carpet, is a trip.
Lovely afternoon in Berlin to run a half-marathon - for the first time.
September 28, 2025 at 5:16 PM
Lovely afternoon in Berlin to run a half-marathon - for the first time.
Redbow - after the downpour, at sunset, Bösebrücke
September 11, 2025 at 5:37 PM
Redbow - after the downpour, at sunset, Bösebrücke
Wild yet peaceful city.
September 9, 2025 at 6:30 AM
Wild yet peaceful city.
Parisian doors @taraska.bsky.social
September 3, 2025 at 4:10 PM
Parisian doors @taraska.bsky.social
September 1, 2025 at 6:49 PM
Post an image from your summer that confers peace.
August 30, 2025 at 2:42 PM
Post an image from your summer that confers peace.
60 km bike tour today, along the path of the Berlin Wall from Hermsdorf to Alt Kladow.
August 19, 2025 at 3:21 PM
60 km bike tour today, along the path of the Berlin Wall from Hermsdorf to Alt Kladow.
Still going, but at least now we are cranking. Train #7 is comfortably today’s fastest. Our journey is much easier in a day since this fast line reduced this Munich to Berlin leg to 4 hours.
August 10, 2025 at 8:33 PM
Still going, but at least now we are cranking. Train #7 is comfortably today’s fastest. Our journey is much easier in a day since this fast line reduced this Munich to Berlin leg to 4 hours.
But train #7 will be ICE 1018 to Berlin Gesundbrunnen, one hour later or so.
August 10, 2025 at 5:24 PM
But train #7 will be ICE 1018 to Berlin Gesundbrunnen, one hour later or so.
We made it to Wörgl perfectly on time but our connecting train (train #6, RJ 84 to Munich) is late. Here’s a picture of our little REX3 ready to trundle back to Schwarzach, with the mountains we passed in the background.
August 10, 2025 at 3:38 PM
We made it to Wörgl perfectly on time but our connecting train (train #6, RJ 84 to Munich) is late. Here’s a picture of our little REX3 ready to trundle back to Schwarzach, with the mountains we passed in the background.
Passing the mythical Kitzbühel, of downhill ski championships fame.
August 10, 2025 at 2:40 PM
Passing the mythical Kitzbühel, of downhill ski championships fame.
Train #5 is REX3 to Wörgl. We are making a very beautiful detour around the engineering work near Rosenheim.
August 10, 2025 at 1:19 PM
Train #5 is REX3 to Wörgl. We are making a very beautiful detour around the engineering work near Rosenheim.
Cable cars in Bad Hofgastein.
August 10, 2025 at 12:29 PM
Cable cars in Bad Hofgastein.