Alex Holehouse
@alexholehouse.bsky.social
Associate Prof. at WashU School of Medicine; biophysics/biochem/evolution of intrinsically disordered proteins. How does nature encode function without a stable structure? We work in vivo / in vitro / in silico. He/him.
https://www.holehouselab.com/
https://www.holehouselab.com/
Brief interruption to science:
For the four folks reading this who may be interested, death metal band Emperor is touring the US next spring. If you care, you care 🤘.
For the four folks reading this who may be interested, death metal band Emperor is touring the US next spring. If you care, you care 🤘.
November 21, 2025 at 11:41 PM
Brief interruption to science:
For the four folks reading this who may be interested, death metal band Emperor is touring the US next spring. If you care, you care 🤘.
For the four folks reading this who may be interested, death metal band Emperor is touring the US next spring. If you care, you care 🤘.
Also needs to be said that (as some folks know) our software packages are named after birds, so while I was away, the lab filled my office with birds... ...but honestly... *very* tastefully.
Will stay for the foreseeable future!
Will stay for the foreseeable future!
October 2, 2025 at 4:00 PM
Also needs to be said that (as some folks know) our software packages are named after birds, so while I was away, the lab filled my office with birds... ...but honestly... *very* tastefully.
Will stay for the foreseeable future!
Will stay for the foreseeable future!
Last week, Washington University made the perhaps questionable decision to award me tenure.
September 29, 2025 at 12:53 PM
Last week, Washington University made the perhaps questionable decision to award me tenure.
Our 4th generation protocol book has arrived!!
August 26, 2025 at 1:17 PM
Our 4th generation protocol book has arrived!!
Although the situation in the world right now is Not Good™, it remains such a preposterous privilege to work with such a wonderful bunch of people. Lab happy hour 25/08/01.
August 2, 2025 at 11:52 PM
Although the situation in the world right now is Not Good™, it remains such a preposterous privilege to work with such a wonderful bunch of people. Lab happy hour 25/08/01.
And now with experimentally-derived ultrastructure 😍
doi.org/10.1101/2024...
doi.org/10.1101/2024...
January 16, 2025 at 9:23 PM
And now with experimentally-derived ultrastructure 😍
doi.org/10.1101/2024...
doi.org/10.1101/2024...
For inspiration, here are the papers we discussed in 2023:
December 30, 2024 at 3:02 PM
For inspiration, here are the papers we discussed in 2023:
One or two delays out of DFW this afternoon….
December 24, 2024 at 9:34 PM
One or two delays out of DFW this afternoon….
So this is actually not a DF image at all, just a TL acquisition edited with high contrast to give a DF-like aesthetic. Just sharing it it cos its pretty 😍. Below is the normal composite of BF + FL
December 18, 2024 at 11:47 PM
So this is actually not a DF image at all, just a TL acquisition edited with high contrast to give a DF-like aesthetic. Just sharing it it cos its pretty 😍. Below is the normal composite of BF + FL
Achievement unlocked: we have our own (BF & FL) scope! h/t Aidan for this nice yeastypic taken just ~30 min after installation and setup!
December 18, 2024 at 1:53 AM
Achievement unlocked: we have our own (BF & FL) scope! h/t Aidan for this nice yeastypic taken just ~30 min after installation and setup!
Degradation implies the deterioration from something good to something less good, yet it is hard to imagine where exactly Outlook can go in this context...
November 26, 2024 at 12:13 AM
Degradation implies the deterioration from something good to something less good, yet it is hard to imagine where exactly Outlook can go in this context...
Post a picture you took (no description) to bring some zen to the timeline
November 16, 2024 at 3:35 PM
Post a picture you took (no description) to bring some zen to the timeline
We examined an essential ~200 residue IDR in the nucleosome-organizing protein Abf1 from budding yeast. Our work suggests that IDR-mediated interactions can be driven by varying contributions of sequence- and chemical specificity.
November 10, 2024 at 2:19 PM
We examined an essential ~200 residue IDR in the nucleosome-organizing protein Abf1 from budding yeast. Our work suggests that IDR-mediated interactions can be driven by varying contributions of sequence- and chemical specificity.
Why should you care? Intrinsically disordered regions are ubiquitous across the Tree of Life and have essential functions in many facets of cell biology, yet often appear poorly conserved as assessed by alignment-based metrics. How can this be?
November 10, 2024 at 2:19 PM
Why should you care? Intrinsically disordered regions are ubiquitous across the Tree of Life and have essential functions in many facets of cell biology, yet often appear poorly conserved as assessed by alignment-based metrics. How can this be?
We’re pleased to share our substantially updated (more below on that…) version of our manuscript exploring the relationship between conservation in sequence/function for intrinsically disordered regions (IDRs) www.biorxiv.org/content/10.1...
November 10, 2024 at 2:19 PM
We’re pleased to share our substantially updated (more below on that…) version of our manuscript exploring the relationship between conservation in sequence/function for intrinsically disordered regions (IDRs) www.biorxiv.org/content/10.1...
2024 lab retreat is a wrap
November 8, 2024 at 10:49 PM
2024 lab retreat is a wrap
Have a protein for which you want to predict disordered regions? Why not try metapredict V3?
www.biorxiv.org/content/10.1...
State-of-the-art accuracy, proteome-scale predictions in seconds, command-line tool, webserver, colab notebook, Docker container, etc.
Check it out here:
metapredict.net
www.biorxiv.org/content/10.1...
State-of-the-art accuracy, proteome-scale predictions in seconds, command-line tool, webserver, colab notebook, Docker container, etc.
Check it out here:
metapredict.net
November 7, 2024 at 11:15 PM
Have a protein for which you want to predict disordered regions? Why not try metapredict V3?
www.biorxiv.org/content/10.1...
State-of-the-art accuracy, proteome-scale predictions in seconds, command-line tool, webserver, colab notebook, Docker container, etc.
Check it out here:
metapredict.net
www.biorxiv.org/content/10.1...
State-of-the-art accuracy, proteome-scale predictions in seconds, command-line tool, webserver, colab notebook, Docker container, etc.
Check it out here:
metapredict.net
They grow up so fast 🥹
July 24, 2024 at 12:58 AM
They grow up so fast 🥹
Much more exciting to me is our ability to predict heterotypic interaction between two IDRs (or an IDR and a folded domain surface) with residue resolution, making it simple to build testable hypotheses as to how an IDR may interact with a partner
June 3, 2024 at 5:11 PM
Much more exciting to me is our ability to predict heterotypic interaction between two IDRs (or an IDR and a folded domain surface) with residue resolution, making it simple to build testable hypotheses as to how an IDR may interact with a partner
This approach allows us to do a few things. One of these is we can (qualitatively; lots of caveats) predict full phase diagrams from sequence
June 3, 2024 at 5:10 PM
This approach allows us to do a few things. One of these is we can (qualitatively; lots of caveats) predict full phase diagrams from sequence
Excited to share new work from Garrett Ginell et al where we present an approach for predicting intermolecular interaction driven by disordered regions. We focus on how chemical complementarity can contribute to and even dictate intermolecular interactions
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
June 3, 2024 at 5:09 PM
Excited to share new work from Garrett Ginell et al where we present an approach for predicting intermolecular interaction driven by disordered regions. We focus on how chemical complementarity can contribute to and even dictate intermolecular interactions
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
EMBL cellular mechanisms by phase separation kicks off with bang!
May 14, 2024 at 1:04 PM
EMBL cellular mechanisms by phase separation kicks off with bang!
IDPSeminars back this week! Sign up here--> idpseminars.com
April 2, 2024 at 6:31 PM
IDPSeminars back this week! Sign up here--> idpseminars.com