This collaborative project was conducted across the following institutes. @stowersinstitute.bsky.social @mdanderson.bsk.social @oxfordwomenshealth.bsky.social @cambiochem.bsky.social @ox.ac.uk @cam.ac.uk

Thank you coauthors @shylonatasha.bsky.social @zgrif26.bsky.social @thomsanger.bsky.social @hollfelderlab.bsky.social @swilliamslabox.bsky.social @aussiebiologist.bsky.social @rrbehringer.bsky.social

Through confocal imaging we describe basement membrane deposition in the ovary during folliculogenesis. We can report a double basement membrane deposited on either side of the granulosa cell layer surrounding the follicles.

Exploring the sub-cellular architecture of the germinal bed & stage II follicles, we could analyse the granulosa (g) and theca (th) cell layers surrounding stage II follicles. Primordial follicles (pf) start exhibiting enlarged nuclei and cytoplasm!

Oogenesis takes place in 10 stages. It starts in the oogonial nest - a mitotic stem cell niche within the germinal bed - which then undergo meiosis to form primordial follicles
We identified oogonia and primordial follicles using section H&E staining paired with confocal DAPI/WGA staining!

Congratulations @juliaeckert.bsky.social! Such a great pipeline that will help many researchers doing analysis of 3D epithelial tissues!

6/6
Massive Thanks to co-first @shylonatasha.bsky.social & all coauthors @hollfelderlab.bsky.social @swilliamslabox.bsky.social‬ @aussiebiologist.bsky.social
for our collaboration between
@stowersinstitute.bsky.social @oxford_uni ‪@cam.ac.uk‬ @oxfordwomenshealth.bsky.social‬ @cambiochem.bsky.social

5/6
Lumenogenesis coincides with circular expression of gastrulation marker Brachyury prior to AP-axis formation suggesting a novel role of Brachyury preceding #gastrulation

4/6
We suggest alternative anterior-posterior #patterning regulation in chameleons with anterior marker genes Cerberus expressed in the middle (arrows) and on the posterior (asterisks) of the embryo but not on the anterior side, while Lefty is completely absent.
#AP-axis

3/6
A purse string mechanism through supra-cellular actin cables closes the epiblast lumen dorsally suggesting a novel form of epiblast lumenogenesis and amniotic cavity formation.

2/6
The #squamate chameleon diverges from what has been reported for #chicken pre-gastrulation morphogenesis
Starting off as flat plate the chameleon hollows and adopts a dome-like shape. Hinge points at the side of the dome result in the formation of amniotic folds.
#devbio #evodevo

Had a lovely time presenting my new chameleon study at the UK #EvoDevo meeting on Friday!
Great discussions and really interesting talks!

If there is still space, could I please be added?
I'm working on comparative analysis of embryogenesis across the amniote phylum using non-model organisms.

Congratulations to Svetlana and all coauthors, this is such a cool study!

Congratulations @aaronhgriffing.bsky.social & team!

such cool data!

really impressive imaging and 3D reconstructions!