Matthew Alexander, PhD
@mattmuscleguy.bsky.social
Geneticist, Skeletal Muscle, Drug development, Gene therapies. Non-coding RNA, and Zebrafish Aficionado. Lover of all things muscle. All posts are my own. Instagram @thealexanderlab
Lab website: https://www.uab.edu/medicine/peds/research/division-researc
Lab website: https://www.uab.edu/medicine/peds/research/division-researc
Reposted by Matthew Alexander, PhD
Proteomics-based evaluation of AAV dystrophin gene therapy outcomes in mdx skeletal muscle
insight.jci.org/articles/vie...
insight.jci.org/articles/vie...
JCI Insight -
Proteomics-based evaluation of AAV dystrophin gene therapy outcomes in mdx skeletal muscle
insight.jci.org
November 28, 2025 at 8:56 PM
Proteomics-based evaluation of AAV dystrophin gene therapy outcomes in mdx skeletal muscle
insight.jci.org/articles/vie...
insight.jci.org/articles/vie...
Reposted by Matthew Alexander, PhD
Enhancer regulator MLL4 controls skeletal muscle metabolic efficiency by limiting AMPK-mediated fuel catabolism
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Enhancer regulator MLL4 controls skeletal muscle metabolic efficiency by limiting AMPK-mediated fuel catabolism - Nature Communications
Skeletal muscle is central to whole-body energy balance. Here, Yang et al. show that MLL4-dependent enhancer activation is a key regulatory node for the governance of muscle metabolic efficiency and s...
www.nature.com
November 27, 2025 at 7:22 PM
Enhancer regulator MLL4 controls skeletal muscle metabolic efficiency by limiting AMPK-mediated fuel catabolism
www.nature.com/articles/s41...
www.nature.com/articles/s41...
New #NatureComms paper: Non-canonical roles of Keap1/Nrf2 in regulating quiescence and early activation in adult muscle stem cells. #Myoblue tinyurl.com/5x6r7ffv
https://www.nature.com/articles/s41467-025-65506-4
tinyurl.com
November 26, 2025 at 1:04 PM
New #NatureComms paper: Non-canonical roles of Keap1/Nrf2 in regulating quiescence and early activation in adult muscle stem cells. #Myoblue tinyurl.com/5x6r7ffv
New #SkeletalMuscle paper: Complement-independent pathogenic influences of anti-HMGCR + and anti-SRP + immune-mediated necrotizing myopathy autoantibodies on engineered muscle function. #Myoblue tinyurl.com/6feyda42
Complement-independent pathogenic influences of anti-HMGCR + and anti-SRP + immune-mediated necrotizing myopathy autoantibodies on engineered muscle function - Skeletal Muscle
Background Immune-mediated necrotizing myopathy (IMNM) is a subgroup of idiopathic inflammatory myopathies associated with anti-signal recognition particle (SRP) or anti-3-hydroxy-3-methylglutaryl-CoA...
tinyurl.com
November 26, 2025 at 12:59 PM
New #SkeletalMuscle paper: Complement-independent pathogenic influences of anti-HMGCR + and anti-SRP + immune-mediated necrotizing myopathy autoantibodies on engineered muscle function. #Myoblue tinyurl.com/6feyda42
New #PNASNews paper: Exercise suppresses DEAF1 to normalize mTORC1 activity and reverse muscle aging. #Myoblue tinyurl.com/y5rayxmz
Exercise suppresses DEAF1 to normalize mTORC1 activity and reverse muscle aging | PNAS
Skeletal muscle is essential for movement, respiration, and metabolism, with mTORC1
acting as a key regulator of protein synthesis and degradation....
tinyurl.com
November 25, 2025 at 3:40 PM
New #PNASNews paper: Exercise suppresses DEAF1 to normalize mTORC1 activity and reverse muscle aging. #Myoblue tinyurl.com/y5rayxmz
New #SkeletalMuscle paper: FLASH: innovative integrated enzymatic-fluorescent labeling for automated muscle fiber typing, metabolic and morphometric analysis. #Myoblue tinyurl.com/4cuhffbv
FLASH: innovative integrated enzymatic-fluorescent labeling for automated muscle fiber typing, metabolic and morphometric analysis - Skeletal Muscle
Background Skeletal muscle is a dynamic tissue capable of structural and metabolic remodeling in response to physiological and pathological stimuli. These adaptations are central to understanding the ...
tinyurl.com
November 22, 2025 at 5:22 PM
New #SkeletalMuscle paper: FLASH: innovative integrated enzymatic-fluorescent labeling for automated muscle fiber typing, metabolic and morphometric analysis. #Myoblue tinyurl.com/4cuhffbv
Congrats to Alan Beggs & colleagues for their new #hmg_journal paper: Loss of adenylosuccinate synthetase 1 in mice recapitulates features of ADSS1 myopathy. #Myoblue tinyurl.com/ycysuctx
Validate User
tinyurl.com
November 22, 2025 at 5:21 PM
Congrats to Alan Beggs & colleagues for their new #hmg_journal paper: Loss of adenylosuccinate synthetase 1 in mice recapitulates features of ADSS1 myopathy. #Myoblue tinyurl.com/ycysuctx
New #CellMetabolism paper: Gut microbiome-adipose crosstalk modulates soluble IL-6 receptor influencing exercise responsiveness in glycemic control and insulin sensitivity. #Myoblue tinyurl.com/3b8dtpzd
Gut microbiome-adipose crosstalk modulates soluble IL-6 receptor influencing exercise responsiveness in glycemic control and insulin sensitivity
Wang and Wu et al. identify soluble interleukin-6 receptor (sIL-6R) as a key exerkine
determining the efficacy of exercise in diabetes prevention, which is modulated by
microbiome-dependent leucine th...
tinyurl.com
November 20, 2025 at 7:08 PM
New #CellMetabolism paper: Gut microbiome-adipose crosstalk modulates soluble IL-6 receptor influencing exercise responsiveness in glycemic control and insulin sensitivity. #Myoblue tinyurl.com/3b8dtpzd
New #NatureComms paper: TBC1D1 functions as a negative regulator of satellite cells for muscle regeneration. #Myoblue tinyurl.com/2wk5pkjt
https://www.nature.com/articles/s41467-025-65141-z
tinyurl.com
November 19, 2025 at 11:33 AM
New #NatureComms paper: TBC1D1 functions as a negative regulator of satellite cells for muscle regeneration. #Myoblue tinyurl.com/2wk5pkjt
Reposted by Matthew Alexander, PhD
Excited to share Emma Sutton's recently published Ph.D. research! By using cells, worms and mice, we report that mifepristone, a glucocorticoid receptor anatagonist, alone and in combination with an approved SMN-dependent therapy, has the potential to improve SMA disease pathology.
rdcu.be/eQpXG
rdcu.be/eQpXG
Mifepristone alone and in combination with scAAV9-SMN1 gene therapy improves disease phenotypes in Smn2B/- spinal muscular atrophy mice
Scientific Reports - Mifepristone alone and in combination with scAAV9-SMN1 gene therapy improves disease phenotypes in Smn2B/- spinal muscular atrophy mice
rdcu.be
November 19, 2025 at 11:20 AM
Excited to share Emma Sutton's recently published Ph.D. research! By using cells, worms and mice, we report that mifepristone, a glucocorticoid receptor anatagonist, alone and in combination with an approved SMN-dependent therapy, has the potential to improve SMA disease pathology.
rdcu.be/eQpXG
rdcu.be/eQpXG
Congrats to @chrisfry and colleagues for their new paper: Zinc deficiency contributes to blunted myogenesis in chronic kidney disease | American Journal of Physiology-Cell Physiology | American Physiological Society #Myoblue journals.physiology.org/doi/abs/10.1...
Zinc deficiency contributes to blunted myogenesis in chronic kidney disease | American Journal of Physiology-Cell Physiology | American Physiological Society
Frailty in patients with chronic kidney disease (CKD) greatly exacerbates disease comorbidities and increases probability of death. Prior research underscores molecular alterations in skeletal muscle ...
journals.physiology.org
November 19, 2025 at 11:31 AM
Congrats to @chrisfry and colleagues for their new paper: Zinc deficiency contributes to blunted myogenesis in chronic kidney disease | American Journal of Physiology-Cell Physiology | American Physiological Society #Myoblue journals.physiology.org/doi/abs/10.1...
New @NatMetabolism paper: Impaired cAMP–PKA–CREB1 signalling drives mitochondrial dysfunction in skeletal muscle during cancer cachexia. #Myoblue tinyurl.com/4adh7uy3
https://www.nature.com/articles/s42255-025-01397-5
tinyurl.com
November 17, 2025 at 10:04 PM
New @NatMetabolism paper: Impaired cAMP–PKA–CREB1 signalling drives mitochondrial dysfunction in skeletal muscle during cancer cachexia. #Myoblue tinyurl.com/4adh7uy3
Myself and the rest of the #UABExerciseMed had a great time at the Tornado Alley Muscle Meeting last week. Outstanding talks from #AnnaThalackerMercer #DavisEnglund and their trainees. Kudos to @kevinmurachphd.bsky.social #DavisEnglund and the other organizers for putting on a great meeting.
November 17, 2025 at 9:31 PM
Myself and the rest of the #UABExerciseMed had a great time at the Tornado Alley Muscle Meeting last week. Outstanding talks from #AnnaThalackerMercer #DavisEnglund and their trainees. Kudos to @kevinmurachphd.bsky.social #DavisEnglund and the other organizers for putting on a great meeting.
The FDA's removal and black box warning of non-ambulatory #DMD patients for gene therapy was announced yesterday and will have a huge impact for the clinical development and target populations. Full description with enhanced safety monitoring here: www.fda.gov/news-events/...
FDA Approves New Safety Warning and Revised Indication that Limits Use for Elevidys Following Reports of Fatal Liver Injury
The U.S. Food and Drug Administration today announced it is taking action to approve new labeling submitted by the company that includes the addition of a Boxed Warning, the agency’s most prominent sa...
www.fda.gov
November 15, 2025 at 11:51 AM
The FDA's removal and black box warning of non-ambulatory #DMD patients for gene therapy was announced yesterday and will have a huge impact for the clinical development and target populations. Full description with enhanced safety monitoring here: www.fda.gov/news-events/...
Interesting work from @rudnicki-lab.bsky.social in #NatureComms : Intrinsic dysfunction in muscle stem cells lacking dystrophin begins during secondary myogenesis. #Myoblue tinyurl.com/4byakujj
https://www.nature.com/articles/s41467-025-64999-3
tinyurl.com
November 15, 2025 at 11:37 AM
Interesting work from @rudnicki-lab.bsky.social in #NatureComms : Intrinsic dysfunction in muscle stem cells lacking dystrophin begins during secondary myogenesis. #Myoblue tinyurl.com/4byakujj
Looking forward to attending the upcoming Tornado Alley Muscle Meeting this week hosted by #KevinMurachPhD #DavisEnglund #ZachHettinger, and #Ben Miller. Hopefully no actual tornados 😄#Myoblue
November 12, 2025 at 7:41 PM
Looking forward to attending the upcoming Tornado Alley Muscle Meeting this week hosted by #KevinMurachPhD #DavisEnglund #ZachHettinger, and #Ben Miller. Hopefully no actual tornados 😄#Myoblue
New #NatureComms paper: Alterations in peroxisome-mitochondria interplay in skeletal muscle accelerate muscle dysfunction. #Myoblue tinyurl.com/4knhr7mh
Alterations in peroxisome-mitochondria interplay in skeletal muscle accelerate muscle dysfunction - Nature Communications
The role of peroxisomes in skeletal muscle remains largely unexplored. Here, the authors show that peroxisomal dysfunction and disrupted crosstalk with mitochondria drive age-related muscle decline, u...
tinyurl.com
November 11, 2025 at 1:25 PM
New #NatureComms paper: Alterations in peroxisome-mitochondria interplay in skeletal muscle accelerate muscle dysfunction. #Myoblue tinyurl.com/4knhr7mh
Congrats to #LaitilaJenni & colleagues for their new @JCI_insight paper: Myosin inhibition partially rescues the myofibre proteome in X-linked myotubular myopathy. #Myoblue tinyurl.com/yyuthutn
JCI Insight -
Myosin inhibition partially rescues the myofibre proteome in X-linked myotubular myopathy
tinyurl.com
November 4, 2025 at 5:32 PM
Congrats to #LaitilaJenni & colleagues for their new @JCI_insight paper: Myosin inhibition partially rescues the myofibre proteome in X-linked myotubular myopathy. #Myoblue tinyurl.com/yyuthutn
Reposted by Matthew Alexander, PhD
Muscle sparing through differential nutritional control of three muscle growth mechanisms: how zebrafish larvae deal with starvation
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Muscle sparing through differential nutritional control of three muscle growth mechanisms: how zebrafish larvae deal with starvation
How vertebrate skeletal muscle size is regulated and balanced with body size over the life-course is unclear, but is important for human health and qu…
www.sciencedirect.com
October 30, 2025 at 4:34 PM
Muscle sparing through differential nutritional control of three muscle growth mechanisms: how zebrafish larvae deal with starvation
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
New #stemcellreports paper: Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSC-derived myogenic progenitor cells. #Myoblue tinyurl.com/yyjhn94p
Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSC-derived myogenic progenitor cells
In this manuscript, Sacco, Caputo, and colleagues demonstrate that modulation of the
JAK2/STAT3 signaling pathway during in vitro myogenic differentiation promotes expansion
of PAX7+ SMPCs in multiple...
tinyurl.com
October 30, 2025 at 4:07 PM
New #stemcellreports paper: Modulation of the JAK2-STAT3 pathway promotes expansion and maturation of human iPSC-derived myogenic progenitor cells. #Myoblue tinyurl.com/yyjhn94p
New #SkeletalMuscle paper: TET exhibits enzymatic-independent and-dependent functions during Drosophila flight muscle development and aging. #Myoblue tinyurl.com/7maz5wsy
TET exhibits enzymatic-independent and-dependent functions during Drosophila flight muscle development and aging - Skeletal Muscle
Background Enzymes of the Ten-Eleven Translocation family are responsible for 5-methylcytosine (5mC) oxidation and play a key role in regulating DNA demethylation during various developmental processe...
tinyurl.com
October 28, 2025 at 12:58 PM
New #SkeletalMuscle paper: TET exhibits enzymatic-independent and-dependent functions during Drosophila flight muscle development and aging. #Myoblue tinyurl.com/7maz5wsy
New #MolTherapy paper: Dose- and genotype-dependent cardiac arrhythmia and sudden death in rats following microdystrophin gene therapy. #DMD #Myoblue tinyurl.com/4f5tj2xn
Dose- and genotype-dependent cardiac arrhythmia and sudden death in rats following microdystrophin gene therapy
High-dose microdystrophin gene therapy improves muscle pathology in DMDmdx rats but
can induce dose-dependent cardiac toxicity, including arrhythmias and sudden death,
in both DMDmdx and wild-type rat...
tinyurl.com
October 23, 2025 at 8:33 PM
New #MolTherapy paper: Dose- and genotype-dependent cardiac arrhythmia and sudden death in rats following microdystrophin gene therapy. #DMD #Myoblue tinyurl.com/4f5tj2xn
New #stemcellreports paper: Delineating transcriptomic signatures of in vitro human skeletal muscle models in comparison to in vivo references. #Myoblue tinyurl.com/mphh5u6j
Delineating transcriptomic signatures of in vitro human skeletal muscle models in comparison to in vivo references
In this article, Yilmaz and colleagues perform a large-scale transcriptomics metaanalysis
with over 400 samples comparing established in vitro skeletal muscle models to in
vivo skeletal muscle samples...
tinyurl.com
October 23, 2025 at 8:27 PM
New #stemcellreports paper: Delineating transcriptomic signatures of in vitro human skeletal muscle models in comparison to in vivo references. #Myoblue tinyurl.com/mphh5u6j
Interesting new #ScienceAdvances paper: Lysosomal damage is a therapeutic target in Duchenne muscular dystrophy. #Myoblue tinyurl.com/mu2dvhc5
Lysosomal damage is a therapeutic target in Duchenne muscular dystrophy
Lysosomal damage contributes to DMD pathology and is a therapeutic target worth considering for current and future approaches.
tinyurl.com
October 23, 2025 at 1:42 PM
Interesting new #ScienceAdvances paper: Lysosomal damage is a therapeutic target in Duchenne muscular dystrophy. #Myoblue tinyurl.com/mu2dvhc5
New #MolTherapyMethClinDev paper on: Enhanced therapeutic potential of a microdystrophin with an extended C-terminal domain. #Myoblue tinyurl.com/y43m53h5
Enhanced therapeutic potential of a microdystrophin with an extended C-terminal domain
Microdystrophins are miniaturized versions of dystrophin, used in AAV gene therapy
of Duchenne muscular dystrophy. Danos and colleagues describe an enhanced microdystrophin
with a C-terminal extension...
tinyurl.com
October 23, 2025 at 1:41 PM
New #MolTherapyMethClinDev paper on: Enhanced therapeutic potential of a microdystrophin with an extended C-terminal domain. #Myoblue tinyurl.com/y43m53h5