Zeshi Li (Jack)
@lizeshi.bsky.social
Assistant Professor, Chemical Biology and Drug Discovery, Utrecht U. PhD G.-J.Boons Lab; Post-doc C.Joo Lab TUDelft. https://www.uu.nl/medewerkers/ZLi2
Reposted by Zeshi Li (Jack)
Now finally peer-reviewed and published in @springernature.com Experimental & Molecular Medicine (EMM)🥳:
www.nature.com/articles/s12...
Our story was published back-to-back with the intriguing paper by Kim et al.:
www.nature.com/articles/s12...
www.nature.com/articles/s12...
Our story was published back-to-back with the intriguing paper by Kim et al.:
www.nature.com/articles/s12...
November 18, 2025 at 11:05 AM
Now finally peer-reviewed and published in @springernature.com Experimental & Molecular Medicine (EMM)🥳:
www.nature.com/articles/s12...
Our story was published back-to-back with the intriguing paper by Kim et al.:
www.nature.com/articles/s12...
www.nature.com/articles/s12...
Our story was published back-to-back with the intriguing paper by Kim et al.:
www.nature.com/articles/s12...
Had the pleasure to learn about this cool platform over a Friday afternoon seminar during Matt’s visit at UU.
Excited to share our laboratory's research tools to study Siglecs along with some interesting discoveries with these tools. Lot of help from many group, but @zeinab-jc.bsky.social was the major driver of this work. Please reach out if you would like to try them out. www.science.org/doi/10.1126/...
An ultrasensitive and modular platform to detect Siglec ligands and control immune cell function
Siglec-liposomes are a multivalent and modular platform for profiling Siglec ligands and modulating immune cell function.
www.science.org
November 17, 2025 at 7:23 PM
Had the pleasure to learn about this cool platform over a Friday afternoon seminar during Matt’s visit at UU.
Reposted by Zeshi Li (Jack)
New @angewandtechemie.bsky.social paper jointly supervised by Zeshi Li @lizeshi.bsky.social and Geert-Jan Boons describes the chemoenzymatic synthesis of O-acetylated GD3 as intact glycolipids, and the use there of to study virus binding in cellular context.
onlinelibrary.wiley.com/doi/10.1002/...
onlinelibrary.wiley.com/doi/10.1002/...
Chemoenzymatically Synthesized O‐Acetylated GD3 Gangliosides to Examine Viral Receptor Specificities in a Cellular Context
7-O-, 9-O-, and 7,9-di-O-acetylated GD3 gangliosides can be prepared by chemical assembly of a tetrasaccharide as α-glycosyl fluoride that is coupled to sphingosine using a glycosynthase, followed by...
onlinelibrary.wiley.com
November 15, 2025 at 12:43 AM
New @angewandtechemie.bsky.social paper jointly supervised by Zeshi Li @lizeshi.bsky.social and Geert-Jan Boons describes the chemoenzymatic synthesis of O-acetylated GD3 as intact glycolipids, and the use there of to study virus binding in cellular context.
onlinelibrary.wiley.com/doi/10.1002/...
onlinelibrary.wiley.com/doi/10.1002/...
#glycotime We hope our b2b publications serve as a heads-up for the #glycoRNA community, and drive the field forward constructively. Links👇
Kim et al. rdcu.be/eP4GQ
@nathanaelbkegel.bsky.social Kegel et al. nature.com/articles/s12276-025-01575-1 (Stefan Bauer lab)
Kim et al. rdcu.be/eP4GQ
@nathanaelbkegel.bsky.social Kegel et al. nature.com/articles/s12276-025-01575-1 (Stefan Bauer lab)
November 15, 2025 at 3:38 PM
#glycotime We hope our b2b publications serve as a heads-up for the #glycoRNA community, and drive the field forward constructively. Links👇
Kim et al. rdcu.be/eP4GQ
@nathanaelbkegel.bsky.social Kegel et al. nature.com/articles/s12276-025-01575-1 (Stefan Bauer lab)
Kim et al. rdcu.be/eP4GQ
@nathanaelbkegel.bsky.social Kegel et al. nature.com/articles/s12276-025-01575-1 (Stefan Bauer lab)
Reposted by Zeshi Li (Jack)
Very happy that NWO supports this major upgrade of our national EM infrastructure. It is great that this initiative got broad support all over NL, and it will EMPower fantastic science - looking forward to that!
www.uu.nl/en/news/33-m...
www.uu.nl/en/news/33-m...
33 million euro investment for national electron microscope facility EMPower
A major new national facility for electron microscopy, called EMPower, has been awarded to a consortium of Dutch universities and medical centers. EMPower aims to pave the way for major discoveries im...
www.uu.nl
November 4, 2025 at 7:14 AM
Very happy that NWO supports this major upgrade of our national EM infrastructure. It is great that this initiative got broad support all over NL, and it will EMPower fantastic science - looking forward to that!
www.uu.nl/en/news/33-m...
www.uu.nl/en/news/33-m...
Reposted by Zeshi Li (Jack)
Thrilled, and admittedly relieved, to see this major investment in Dutch #cryoEM become reality! 🇳🇱🔬
Honoured to be a co-applicant on this national initiative and very excited for the opportunity to advance our work in cellular structural virology! 🦠
www.uu.nl/en/news/33-m...
Honoured to be a co-applicant on this national initiative and very excited for the opportunity to advance our work in cellular structural virology! 🦠
www.uu.nl/en/news/33-m...
33 million euro investment for national electron microscope facility EMPower
A major new national facility for electron microscopy, called EMPower, has been awarded to a consortium of Dutch universities and medical centers. EMPower aims to pave the way for major discoveries im...
www.uu.nl
November 3, 2025 at 4:47 PM
Thrilled, and admittedly relieved, to see this major investment in Dutch #cryoEM become reality! 🇳🇱🔬
Honoured to be a co-applicant on this national initiative and very excited for the opportunity to advance our work in cellular structural virology! 🦠
www.uu.nl/en/news/33-m...
Honoured to be a co-applicant on this national initiative and very excited for the opportunity to advance our work in cellular structural virology! 🦠
www.uu.nl/en/news/33-m...
Deeply honored to be a co-applicant of #BioBeyond_NL. The team will develop enabling #MassSpec based technologies to advance (glyco)proteomics, glycomics and spatial omics.
Stay tuned for PhD/post-doc openings in the near future!
www.nwo.nl/en/news/nati...
Stay tuned for PhD/post-doc openings in the near future!
www.nwo.nl/en/news/nati...
National Roadmap: NWO invests 197 million euro in eleven Large-Scale Research Infrastructures | NWO
Eleven consortia from various scientific disciplines are set to launch projects of great value to science. But also to the economic and social prosperity of the Netherlands. For example, a consortium led by TU Delft will start work on a detailed virtual model of the Dutch energy system. In another project, a consortium of UMC Utrecht is developing an MRI scanner that can scan people in motion.
www.nwo.nl
November 3, 2025 at 7:20 PM
Deeply honored to be a co-applicant of #BioBeyond_NL. The team will develop enabling #MassSpec based technologies to advance (glyco)proteomics, glycomics and spatial omics.
Stay tuned for PhD/post-doc openings in the near future!
www.nwo.nl/en/news/nati...
Stay tuned for PhD/post-doc openings in the near future!
www.nwo.nl/en/news/nati...
Reposted by Zeshi Li (Jack)
Congratulations to CBDD researchers, Geert-Jan Boons and Zeshi Li @lizeshi.bsky.social, and members of #BioBeyond_NL, a consortium co-led by Maastricht, Utrecht, Radboud University and LUMC researchers to advance (glyco)proteomics, glycomics and spatial omics. Read more👇
www.nwo.nl/en/news/nati...
www.nwo.nl/en/news/nati...
National Roadmap: NWO invests 197 million euro in eleven Large-Scale Research Infrastructures | NWO
Eleven consortia from various scientific disciplines are set to launch projects of great value to science. But also to the economic and social prosperity of the Netherlands. For example, a consortium led by TU Delft will start work on a detailed virtual model of the Dutch energy system. In another project, a consortium of UMC Utrecht is developing an MRI scanner that can scan people in motion.
www.nwo.nl
November 3, 2025 at 6:58 PM
Congratulations to CBDD researchers, Geert-Jan Boons and Zeshi Li @lizeshi.bsky.social, and members of #BioBeyond_NL, a consortium co-led by Maastricht, Utrecht, Radboud University and LUMC researchers to advance (glyco)proteomics, glycomics and spatial omics. Read more👇
www.nwo.nl/en/news/nati...
www.nwo.nl/en/news/nati...
Reposted by Zeshi Li (Jack)
Advancing #glycoRNA imaging with FRET-based detection and expanding the scope of their presentation to extracellular vesicles - interestingly high levels of sialoglycoRNAs on blood EVs can be predictive of having cancer @naturecomms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
www.nature.com
April 11, 2025 at 2:53 PM
Advancing #glycoRNA imaging with FRET-based detection and expanding the scope of their presentation to extracellular vesicles - interestingly high levels of sialoglycoRNAs on blood EVs can be predictive of having cancer @naturecomms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Zeshi Li (Jack)
SCAM ALERT. This is a fake conference that ripped off my and other people’s names to trick people into registering. There is no such conference that I or the others shown were invited or agreed to. We are working to try to get this fraud taken down from the web.
chemsymposia.com
chemsymposia.com
Join Chemsymposia 2026: Research & Innovation | ChemSymposia 2026
Join us at Chemsymposia 2026 to discover groundbreaking research in physics applications. Connect with experts, engage in insightful discussions, and explore our venue, registration details, and speak...
chemsymposia.com
April 11, 2025 at 4:35 PM
SCAM ALERT. This is a fake conference that ripped off my and other people’s names to trick people into registering. There is no such conference that I or the others shown were invited or agreed to. We are working to try to get this fraud taken down from the web.
chemsymposia.com
chemsymposia.com
Reposted by Zeshi Li (Jack)
Question for glycoRNA proponents: What specific evidence would convince you that these signals are protein contaminants rather than modified RNA? Since so much evidence depends on extraction & analysis of pure RNase-sensitive glycoRNA, this seems like a problem.
Worth reading the whole exchange ⬇️
Worth reading the whole exchange ⬇️
Second preprint casting doubt on glycoRNA.
“we further demonstrate that recombinant soluble LAMP1 can be purified following the glycoRNA isolation method… thus representing a considerable source of glycans in samples of glycoRNA”
Still waiting for a journal publication of either preprint.
“we further demonstrate that recombinant soluble LAMP1 can be purified following the glycoRNA isolation method… thus representing a considerable source of glycans in samples of glycoRNA”
Still waiting for a journal publication of either preprint.
March 30, 2025 at 2:38 PM
Question for glycoRNA proponents: What specific evidence would convince you that these signals are protein contaminants rather than modified RNA? Since so much evidence depends on extraction & analysis of pure RNase-sensitive glycoRNA, this seems like a problem.
Worth reading the whole exchange ⬇️
Worth reading the whole exchange ⬇️
Here is an updated version of a preprint posted in 2024: It describes a non-RNA glycoconjugate persisted throughout RNA sample processing, with similar electrophoretic properties and enzymatic reactivity as glycoRNA. It confounds gel-/blot-based glycoRNA assays.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
RNA-Associated Glycoconjugates Highlight Potential Ambiguities in glycoRNA Analysis
A recent ground-breaking study suggested that small RNA from mammalian cells can undergo N-glycan modifications (termed glycoRNA) 1. The discovery relied upon a metabolic glycan labeling strategy in c...
www.biorxiv.org
March 28, 2025 at 2:33 PM
Here is an updated version of a preprint posted in 2024: It describes a non-RNA glycoconjugate persisted throughout RNA sample processing, with similar electrophoretic properties and enzymatic reactivity as glycoRNA. It confounds gel-/blot-based glycoRNA assays.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Zeshi Li (Jack)
Nanopore technology might be able to sequence proteins soon! Read about the road ahead in this exciting Perspective rdcu.be/edWPi in @naturebiotech.bsky.social
Toward single-molecule protein sequencing using nanopores
Nature Biotechnology - Maglia and colleagues discuss advances in nanopore technology en route to single-molecule protein sequencing
rdcu.be
March 18, 2025 at 10:18 AM
Nanopore technology might be able to sequence proteins soon! Read about the road ahead in this exciting Perspective rdcu.be/edWPi in @naturebiotech.bsky.social
Reposted by Zeshi Li (Jack)
We're just completing an R&D sprint to identify an LC additive that increases our glycan identifications 3x and enhances glycan charge states by up to 45%🚀.
It's too early to publicly disclose its identity, but here's a results preview.
Interested? Message or email us, we'd love to collaborate.
It's too early to publicly disclose its identity, but here's a results preview.
Interested? Message or email us, we'd love to collaborate.
March 24, 2025 at 11:16 AM
We're just completing an R&D sprint to identify an LC additive that increases our glycan identifications 3x and enhances glycan charge states by up to 45%🚀.
It's too early to publicly disclose its identity, but here's a results preview.
Interested? Message or email us, we'd love to collaborate.
It's too early to publicly disclose its identity, but here's a results preview.
Interested? Message or email us, we'd love to collaborate.
Reposted by Zeshi Li (Jack)
Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
March 24, 2025 at 3:56 PM
Excited to share our new preprint on co-transcriptional splicing! U1 snRNP is recruited to RNA polymerase II through multiple interaction sites, with elongation factor SPT6 binding directly to the U1 snRNP protein.
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
www.biorxiv.org/content/10.1...
#cryoEM #transcription #splicing
Excited to share an update on heparan sulfate (HS)-associated cell-surface RNA (hepRNA):
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Cell-Surface RNA Forms Ternary Complex with RNA-Binding Proteins and Heparan Sulfate to Recruit Immune Receptors
Recent discoveries have shown the presence of RNA molecules on the cell surface, defying the traditional view that RNA only functions intracellularly. However, it is not well understood how cell-surfa...
www.biorxiv.org
March 24, 2025 at 3:18 PM
Excited to share an update on heparan sulfate (HS)-associated cell-surface RNA (hepRNA):
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Zeshi Li (Jack)
Transcriptome-wide Identification of GlycoRNAs by Clier-seq Pipeline https://www.biorxiv.org/content/10.1101/2025.02.28.639800v1
March 1, 2025 at 1:16 AM
Transcriptome-wide Identification of GlycoRNAs by Clier-seq Pipeline https://www.biorxiv.org/content/10.1101/2025.02.28.639800v1
Reposted by Zeshi Li (Jack)
Proteins are a source of glycans found in preparations of glycoRNA https://www.biorxiv.org/content/10.1101/2025.03.17.643711v1
March 18, 2025 at 6:17 AM
Proteins are a source of glycans found in preparations of glycoRNA https://www.biorxiv.org/content/10.1101/2025.03.17.643711v1
Reposted by Zeshi Li (Jack)
Hello, Bluesky community! Let our first post be a good news: CBDD researchers Geert-Jan Boons and Rob de Vries (@rpdevrieslab.bsky.social), together with researchers in ErasmusMC Rotterdam, were awarded an #ENW-XL from the Dutch Research Council. Stay tuned for #phd/#post-doc openings! #glycotime
December 18, 2024 at 11:39 AM
Hello, Bluesky community! Let our first post be a good news: CBDD researchers Geert-Jan Boons and Rob de Vries (@rpdevrieslab.bsky.social), together with researchers in ErasmusMC Rotterdam, were awarded an #ENW-XL from the Dutch Research Council. Stay tuned for #phd/#post-doc openings! #glycotime