Kresten Lindorff-Larsen
@lindorfflarsen.bsky.social
Protein and coffee lover, father of two, professor of biophysics and sudo scientist at the Linderstrøm-Lang Centre for Protein Science, University of Copenhagen 🇩🇰
Pinned
We (@sobuelow.bsky.social) developed AF-CALVADOS to integrate AlphaFold and CALVADOS to simulate flexible multidomain proteins at scale
See preprint for:
— Ensembles of >12000 full-length human proteins
— Analysis of IDRs in >1500 TFs
📜 doi.org/10.1101/2025...
💾 github.com/KULL-Centre/...
See preprint for:
— Ensembles of >12000 full-length human proteins
— Analysis of IDRs in >1500 TFs
📜 doi.org/10.1101/2025...
💾 github.com/KULL-Centre/...
Reposted by Kresten Lindorff-Larsen
Energy transitions can happen faster than we think:
In 2000 almost 90% of Denmark's electricity was from fossil fuels.
In 2024 less than 10% of Danish electricity was from fossil fuels.
In 2000 almost 90% of Denmark's electricity was from fossil fuels.
In 2024 less than 10% of Danish electricity was from fossil fuels.
December 1, 2025 at 4:18 PM
Energy transitions can happen faster than we think:
In 2000 almost 90% of Denmark's electricity was from fossil fuels.
In 2024 less than 10% of Danish electricity was from fossil fuels.
In 2000 almost 90% of Denmark's electricity was from fossil fuels.
In 2024 less than 10% of Danish electricity was from fossil fuels.
Reposted by Kresten Lindorff-Larsen
Love this; inspired by early experiences and cemented by the incomparable @jiwasa.bsky.social at an incredible workshop she and @theroglab.bsky.social organized in '22, I've become thoroughly convinced that - at least for me - developing figures from scratch is a key part of scientific inquiry.
The hidden danger of Biorender
(& the death of scientific illustration)
A short thread 🧵
(& the death of scientific illustration)
A short thread 🧵
December 1, 2025 at 1:04 PM
Love this; inspired by early experiences and cemented by the incomparable @jiwasa.bsky.social at an incredible workshop she and @theroglab.bsky.social organized in '22, I've become thoroughly convinced that - at least for me - developing figures from scratch is a key part of scientific inquiry.
Happy December 1st
December 1, 2025 at 8:34 PM
Happy December 1st
"Ok, imagine this. What if a medical diagnosis didn't just give you an answer, but also gave you a clear, easy to understand explanation of why"
If you didn't understand the paper below, you can watch this explainer video (or the first 3 minutes at least), for some more made-up AI-generated stuff
If you didn't understand the paper below, you can watch this explainer video (or the first 3 minutes at least), for some more made-up AI-generated stuff
November 28, 2025 at 9:27 PM
"Ok, imagine this. What if a medical diagnosis didn't just give you an answer, but also gave you a clear, easy to understand explanation of why"
If you didn't understand the paper below, you can watch this explainer video (or the first 3 minutes at least), for some more made-up AI-generated stuff
If you didn't understand the paper below, you can watch this explainer video (or the first 3 minutes at least), for some more made-up AI-generated stuff
Reposted by Kresten Lindorff-Larsen
This suggests that FtsZ's disordered C-terminal linker may encode geometric information and that this could have an evolutionary role!
November 26, 2025 at 9:29 PM
This suggests that FtsZ's disordered C-terminal linker may encode geometric information and that this could have an evolutionary role!
Reposted by Kresten Lindorff-Larsen
Happy to share a new preprint from the lab: Marko became interested in whether the disordered linker of FtsZ might influence how the protein organizes into the Z-ring. And this led to some surprising findings!
Preprint: www.biorxiv.org/content/10.1...
Preprint: www.biorxiv.org/content/10.1...
Charge segregation in the C-terminal linker of FtsZ enables Z-ring scaling with prokaryotic cell width
Bacterial cytokinesis is orchestrated by the Z-ring, a cytoskeletal structure formed by treadmilling filaments of the tubulin-like GTPase FtsZ. During assembly, filament curvature must match the cell ...
www.biorxiv.org
November 26, 2025 at 9:29 PM
Happy to share a new preprint from the lab: Marko became interested in whether the disordered linker of FtsZ might influence how the protein organizes into the Z-ring. And this led to some surprising findings!
Preprint: www.biorxiv.org/content/10.1...
Preprint: www.biorxiv.org/content/10.1...
STOP THE COUNT!
November 26, 2025 at 10:03 PM
STOP THE COUNT!
Reposted by Kresten Lindorff-Larsen
All wiggly protein afficionados 🍝 - The IDP grodon conf is filling up fast. Please submit your registration ASAP! The conference takes place June 21-27 2026 in Les Diablerets, Switzerland, with an amazing speaker lineup! Details: tinyurl.com/yfm5jsxt @idpgrc.bsky.social @idpseminars.bsky.social
2026 Intrinsically Disordered Proteins Conference GRC
The 2026 Gordon Research Conference on Intrinsically Disordered Proteins will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.
www.grc.org
November 26, 2025 at 6:04 PM
All wiggly protein afficionados 🍝 - The IDP grodon conf is filling up fast. Please submit your registration ASAP! The conference takes place June 21-27 2026 in Les Diablerets, Switzerland, with an amazing speaker lineup! Details: tinyurl.com/yfm5jsxt @idpgrc.bsky.social @idpseminars.bsky.social
Reposted by Kresten Lindorff-Larsen
TTPD Episode 9 with @fraserlab.com: I Can Do It With a Broken Grant Cycle
(Sorry for the crappy audio in places!)
Topics: Government Shutdown/Reopening, Travels, Jane Richardson's impact (en.wikipedia.org/wiki/Jane_S....), Arena BioWorks closing, AI review tools
wankowiczlab.com/2025/11/24/T...
(Sorry for the crappy audio in places!)
Topics: Government Shutdown/Reopening, Travels, Jane Richardson's impact (en.wikipedia.org/wiki/Jane_S....), Arena BioWorks closing, AI review tools
wankowiczlab.com/2025/11/24/T...
November 24, 2025 at 3:22 PM
TTPD Episode 9 with @fraserlab.com: I Can Do It With a Broken Grant Cycle
(Sorry for the crappy audio in places!)
Topics: Government Shutdown/Reopening, Travels, Jane Richardson's impact (en.wikipedia.org/wiki/Jane_S....), Arena BioWorks closing, AI review tools
wankowiczlab.com/2025/11/24/T...
(Sorry for the crappy audio in places!)
Topics: Government Shutdown/Reopening, Travels, Jane Richardson's impact (en.wikipedia.org/wiki/Jane_S....), Arena BioWorks closing, AI review tools
wankowiczlab.com/2025/11/24/T...
Reposted by Kresten Lindorff-Larsen
Proteins are dynamic structures, but structural biology often shows them as static snapshots. Inspired by long-exposure photography and generative art, I built ProteinCHAOS, an artistic tool inspired by molecular dynamics to capture protein flexibility over time, much like long-exposure images.
November 23, 2025 at 11:18 PM
Proteins are dynamic structures, but structural biology often shows them as static snapshots. Inspired by long-exposure photography and generative art, I built ProteinCHAOS, an artistic tool inspired by molecular dynamics to capture protein flexibility over time, much like long-exposure images.
One of these is Figure 1 from my first paper and the other is an image generated using Nano Banana Pro solely from the caption of Figure 1 of my first paper
![[Figure generated using AI] Progress curves for the reaction between GSH (Merck) and 2-VP (Aldrich). 0.2 mM 2-VP was incubated with 5 mM (circles), 10 mM (squares), 15 mM (diamonds), and 20 mM (triangles) GSH at pH 6.8, and 25°C. At the indicated times, 20 l were withdrawn and injected into a Vydac C18 column on a Hewlett–Packard HPLC system running at 1 ml/min with 0.1% trifluoroacetic acid in 10% acetonitrile. The reaction could be followed using online fluorescence detection ( ex 254 nm, em 340 nm), since free 2-VP fluoresces much more that its GSH conjugate. The solid lines represent nonlinear least-squares fit to a single exponential function. (Inset) Pseudo-first-order rate constants are plotted against glutathione concentration. The slope of the straight line is an estimate of the second-order rate constant at pH 6.8.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:av5rwmfzfvex2hquvjxtlqlz/bafkreicvkeu6oucxhtcjppmro3xl4k7io6dkgvspiqfnjhm2ad34lt52am@jpeg)
![[Figure from my first paper with the caption] Progress curves for the reaction between GSH (Merck) and 2-VP (Aldrich). 0.2 mM 2-VP was incubated with 5 mM (circles), 10 mM (squares), 15 mM (diamonds), and 20 mM (triangles) GSH at pH 6.8, and 25°C. At the indicated times, 20 l were withdrawn and injected into a Vydac C18 column on a Hewlett–Packard HPLC system running at 1 ml/min with 0.1% trifluoroacetic acid in 10% acetonitrile. The reaction could be followed using online fluorescence detection ( ex 254 nm, em 340 nm), since free 2-VP fluoresces much more that its GSH conjugate. The solid lines representnonlinear least-squares fit to a single exponential function. (Inset) Pseudo-first-order rate constants are plotted against glutathione concentration. The slope of the straight line is an estimate of the second-order rate constant at pH 6.8.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:av5rwmfzfvex2hquvjxtlqlz/bafkreiesuag34bsz45mfvir6fxrotzs6dqn5kss4mlygacwumgm4odtl6y@jpeg)
November 23, 2025 at 9:18 PM
One of these is Figure 1 from my first paper and the other is an image generated using Nano Banana Pro solely from the caption of Figure 1 of my first paper
Reposted by Kresten Lindorff-Larsen
Italian researchers (and Batman) conducted a field study on the Milan metro (138 rides)... ⬇️
"Passengers were significantly more likely to offer their seat when Batman was present (67.21% vs. 37.66%, OR = 3.393, p < 0.001)"
I can smell IgNobel
pmc.ncbi.nlm.nih.gov/articles/PMC...
"Passengers were significantly more likely to offer their seat when Batman was present (67.21% vs. 37.66%, OR = 3.393, p < 0.001)"
I can smell IgNobel
pmc.ncbi.nlm.nih.gov/articles/PMC...
a close up of a cartoon character wearing a batman costume .
ALT: a close up of a cartoon character wearing a batman costume .
media.tenor.com
November 23, 2025 at 5:24 PM
Italian researchers (and Batman) conducted a field study on the Milan metro (138 rides)... ⬇️
"Passengers were significantly more likely to offer their seat when Batman was present (67.21% vs. 37.66%, OR = 3.393, p < 0.001)"
I can smell IgNobel
pmc.ncbi.nlm.nih.gov/articles/PMC...
"Passengers were significantly more likely to offer their seat when Batman was present (67.21% vs. 37.66%, OR = 3.393, p < 0.001)"
I can smell IgNobel
pmc.ncbi.nlm.nih.gov/articles/PMC...
Reposted by Kresten Lindorff-Larsen
New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!
November 19, 2025 at 9:16 PM
New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!
Reposted by Kresten Lindorff-Larsen
Jeg står her omkring i morgen fra ca. kl 10 og frem til 11:30 eller der omkring. Så går turen videre til Panum. Håber vi ses!
November 16, 2025 at 7:53 PM
Jeg står her omkring i morgen fra ca. kl 10 og frem til 11:30 eller der omkring. Så går turen videre til Panum. Håber vi ses!
Reposted by Kresten Lindorff-Larsen
Global Analysis of Aggregation Determinants in Small Protein Domains https://www.biorxiv.org/content/10.1101/2025.11.11.687847v1
November 13, 2025 at 1:47 AM
Global Analysis of Aggregation Determinants in Small Protein Domains https://www.biorxiv.org/content/10.1101/2025.11.11.687847v1
Reposted by Kresten Lindorff-Larsen
Since you ask.
From the birth of quantum to the birth of CERN.
From the birth of quantum to the birth of CERN.
November 13, 2025 at 12:43 PM
Since you ask.
From the birth of quantum to the birth of CERN.
From the birth of quantum to the birth of CERN.
Reposted by Kresten Lindorff-Larsen
My first full contribution from my time in @opig.stats.ox.ac.uk is now out! Together with @fspoendlin.bsky.social (and with contributions from King Ifashe), we created FlAbDab and FTCRDab: two large-scale, open molecular dynamics datasets to study flexibility in immune receptors.
November 12, 2025 at 9:21 PM
My first full contribution from my time in @opig.stats.ox.ac.uk is now out! Together with @fspoendlin.bsky.social (and with contributions from King Ifashe), we created FlAbDab and FTCRDab: two large-scale, open molecular dynamics datasets to study flexibility in immune receptors.
😱 I worry the next step is to allow spaces in filenames
No no no begs every archivist. You are never going to be able to find anything. Please don’t start using emojis in file names. Who asked for this? What fresh hell is next?
November 12, 2025 at 8:21 PM
😱 I worry the next step is to allow spaces in filenames
Reposted by Kresten Lindorff-Larsen
Uncovering the flexibility of CDR loops in antibodies and TCRs through large-scale molecular dynamics https://www.biorxiv.org/content/10.1101/2025.11.10.687725v1
November 12, 2025 at 7:48 PM
Uncovering the flexibility of CDR loops in antibodies and TCRs through large-scale molecular dynamics https://www.biorxiv.org/content/10.1101/2025.11.10.687725v1
Reposted by Kresten Lindorff-Larsen
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
November 5, 2025 at 4:23 PM
Excited to share our latest @nature.com: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation.💊🧪 🧵👇 www.nature.com/articles/s41...
Reposted by Kresten Lindorff-Larsen
🎙️ Next up Dec 2 in VESS!
Thea Schulze (Lindorff-Larsen Lab): Predicting mutated protein abundance @tkschulze.bsky.social
Taylor Mighell (Lehner Lab): Massive mutagenesis to understand GPCRs @taylor-mighell.bsky.social
🔗 More info at varianteffect.org/seminar-series
@varianteffect.bsky.social
Thea Schulze (Lindorff-Larsen Lab): Predicting mutated protein abundance @tkschulze.bsky.social
Taylor Mighell (Lehner Lab): Massive mutagenesis to understand GPCRs @taylor-mighell.bsky.social
🔗 More info at varianteffect.org/seminar-series
@varianteffect.bsky.social
November 4, 2025 at 6:31 PM
🎙️ Next up Dec 2 in VESS!
Thea Schulze (Lindorff-Larsen Lab): Predicting mutated protein abundance @tkschulze.bsky.social
Taylor Mighell (Lehner Lab): Massive mutagenesis to understand GPCRs @taylor-mighell.bsky.social
🔗 More info at varianteffect.org/seminar-series
@varianteffect.bsky.social
Thea Schulze (Lindorff-Larsen Lab): Predicting mutated protein abundance @tkschulze.bsky.social
Taylor Mighell (Lehner Lab): Massive mutagenesis to understand GPCRs @taylor-mighell.bsky.social
🔗 More info at varianteffect.org/seminar-series
@varianteffect.bsky.social
Reposted by Kresten Lindorff-Larsen
*If you would like to be considered for a talk at this workshop, please register & submit an abstract by 7 November*
We also have a fantastic line-up of invited speakers: Matteo Degiacomi, Lucie Delemotte, Weria Pezeshkian, Mohsen Sadeghi & Ilpo Vattulainen
Registration: aias.au.dk/events/show/...
We also have a fantastic line-up of invited speakers: Matteo Degiacomi, Lucie Delemotte, Weria Pezeshkian, Mohsen Sadeghi & Ilpo Vattulainen
Registration: aias.au.dk/events/show/...
November 4, 2025 at 11:46 AM
*If you would like to be considered for a talk at this workshop, please register & submit an abstract by 7 November*
We also have a fantastic line-up of invited speakers: Matteo Degiacomi, Lucie Delemotte, Weria Pezeshkian, Mohsen Sadeghi & Ilpo Vattulainen
Registration: aias.au.dk/events/show/...
We also have a fantastic line-up of invited speakers: Matteo Degiacomi, Lucie Delemotte, Weria Pezeshkian, Mohsen Sadeghi & Ilpo Vattulainen
Registration: aias.au.dk/events/show/...
Reposted by Kresten Lindorff-Larsen
Probing the energy landscape of α-Synuclein amyloid fibril formation by systematic K-to-Q mutagenesis https://www.biorxiv.org/content/10.1101/2025.11.01.685997v1
November 3, 2025 at 10:48 PM
Probing the energy landscape of α-Synuclein amyloid fibril formation by systematic K-to-Q mutagenesis https://www.biorxiv.org/content/10.1101/2025.11.01.685997v1
Reposted by Kresten Lindorff-Larsen
Anyway, we need some joy, so here's the Egyptian foreign minister being given a Lego Pyramid by the Danish foreign minister.
November 3, 2025 at 2:35 PM
Anyway, we need some joy, so here's the Egyptian foreign minister being given a Lego Pyramid by the Danish foreign minister.