Lightnews — Scholar-powered news

Tobias Kroniger @kronigert.bsky.social · 9h

Very happy to hear that! It should be quite stable for "standard" proteomics methods. Can be a bit trickier for stepping or multi-segment methods, but even these should work as far as I have tested

1

Tobias Kroniger @kronigert.bsky.social · 9h

I sure hope that there wont be too many, haha. But I you encounter some, please report them! Happy testing :)

1 1

Tobias Kroniger @kronigert.bsky.social · 10h

Yes, that should be unnecessary now!

1 1

Tobias Kroniger @kronigert.bsky.social · 10h

What things did you adapt via notepad++? The polygon for dda measurements?

1

Tobias Kroniger @kronigert.bsky.social · 12h

Thank you! Yes, also TME only works for same instrument type. timsCompare works instrument independent! Can also be a great tool to recover a dia-PASEF scheme from a PRIDE repository for example.

2

Tobias Kroniger @kronigert.bsky.social · 14h

timsCompare is free, open-source, and available now on GitHub! All feedback, bug reports, and feature ideas are welcome.

github.com/kronigert/ti...

#MassSpec #TeamMassSpec #Proteomics #timsTOF #diaPASEF #diagonalPASEF

(Disclaimer: This is an independent project, not an official Bruker tool.)

GitHub - kronigert/timsCompare: A desktop application for mass spectrometry users to load, view, compare timsTOF methods and export isolation schemes and method reports from Bruker's .d method directo...

A desktop application for mass spectrometry users to load, view, compare timsTOF methods and export isolation schemes and method reports from Bruker's .d method directories. - kronigert/timsCom...

github.com

1 1 6

Tobias Kroniger @kronigert.bsky.social · 14h

Need to document your method for a publication or lab notebook? timsCompare generates professional, multi-page PDF reports with high-quality plots and neatly organized parameter tables. CSV export is also supported for easy data handling.

1 2

Tobias Kroniger @kronigert.bsky.social · 14h

Visualize acquisition schemes for PASEF, dia-PASEF, and diagonal-PASEF methods. You can easily export these window schemes directly from your raw data into a simple text file for documentation. The exported text file can also be read directly by timsControl.

1 1

Tobias Kroniger @kronigert.bsky.social · 14h

Simply drag & drop your .d or .m folders. timsCompare instantly gives you a side-by-side parameter comparison. Any differences between methods are automatically highlighted, so you can spot changes at a glance.

1 1

Tobias Kroniger @kronigert.bsky.social · 14h

Struggling to compare Bruker timsTOF methods or extract window schemes from raw .d files? I developed a free desktop tool to solve this: timsCompare! 🚀

It automates the manual work of extracting method parameters, presenting them in a structured and comparable view.

(A thread 🧵)

2 9 19

Tobias Kroniger @kronigert.bsky.social · Jun 14

Will send you a document via DM! :)

Tobias Kroniger @kronigert.bsky.social · Jun 13

Hi Dorothy, do you mean the valve plumbing or the method setup? I guess you plan to make a calibration segment at the start of each run for external calibration?

1

Tobias Kroniger @kronigert.bsky.social · May 27

If you're not attending ASMS but are interested in updates from Bruker, you can still take part in the eXceed symposia via livestream.

Register here to join the livestream:
www.bruker.com/en/landingpa...

#ASMS2025 #Bruker #MassSpectrometry #eXceedSymposia

ASMS 2025

Join Bruker at ASMS 2025. Experience cutting-edge advancements in mass spectrometry technology as the innovation superheroes come to ASMS 2025.

www.bruker.com

1 3

Tobias Kroniger @kronigert.bsky.social · Apr 4

Do you want to do m/z or IM calibration?

1

Reposted by Tobias Kroniger

Andrea Sinz 🧪 @sinzandrea.bsky.social · Feb 7

Job alert 🧪 ‼️ Hi #teammassspec: In our #RTG2467 @unihalle.bsky.social we have a job opening for a PhD student in my group! 🎉 If you want to do cool stuff in cross-linking mass spectrometry of the tumor suppressor p53, please take a look:
personal.verwaltung.uni-halle.de/jobs/wissmi/
Please RT!

Externe Stellenausschreibungen- Wissenschaftliche Mitarbeiterinnen und Mitarbeiter, Ärzte und Lehrkräfte für besondere Aufgaben

personal.verwaltung.uni-halle.de

2 13 17

Reposted by Tobias Kroniger

Andrea Sinz 🧪 @sinzandrea.bsky.social · Feb 13

🧪If you join our lab you will be able to use our three timsTOF mass spectrometers….and we also got a new microwave oven for our lab kitchen today!😜
#ChemSky
#AcademicSky

2 5 15

Reposted by Tobias Kroniger

Patricia Skowronek @patiskowronek.bsky.social · Jan 20

🚀 Robust and high sensitivity #proteomics: Our Nature protocol demystifies #PASEF workflows and provides ready-to-use dia-PASEF & synchro-PASEF methods. Find out how to achieve 7,000 protein groups or 29,000 phosphosites in 21min. Let's explore! #TeamMassSpec #Bruker doi.org/10.1038/s415... 1/🧵

https://doi.org/10.1038/s41596-024-01104-w1/🧵

4 10 35

Reposted by Tobias Kroniger

Andrea Sinz 🧪 @sinzandrea.bsky.social · Dec 15

TimsTOF ultra 2 cookies for the group.🍪😋🎄🎄🎄 #teammassspec @brukercorporation.bsky.social

2 6 38

Tobias Kroniger @kronigert.bsky.social · Dec 2

There is a disclaimer when you scan the QR-Code. They are built from biodegradable, food-contact-safe-plastic. Please hand-wash only!

1 4

Tobias Kroniger @kronigert.bsky.social · Nov 30

Both options are usable. For SN only directDIA is available, while TIMS DIANN uses a library-based approach

1 3

Tobias Kroniger @kronigert.bsky.social · Nov 29

I think it's very hard to make a fair comparison between instruments. There are just too many variables

1 1

Tobias Kroniger @kronigert.bsky.social · Nov 29

Happy to hear that everything was working out in the end! Crossing fingers for your measurements!

2

Reposted by Tobias Kroniger

Witold Szymański @witek-teammassspec.bsky.social · Nov 26

Just got my TimsTof Ultra cookie form 😏 a nice touch from #bruker

5 5 29

Reposted by Tobias Kroniger

Michael Sinden @mdsinden.bsky.social · Nov 25

@brukercorporation.bsky.social‘s latest app note highlights that ultra-high-throughput proteomics doesn’t have to compromise depth or quality.

Pairing a timsTOF HT with an Aurora Rapid 5x75 column, researchers ID over 7,200 protein groups & 85,658 precursors in 5 mins.

Read: bit.ly/3YWZk27

2 9

Reposted by Tobias Kroniger

Alejandro Brenes @ajbrenes.com · Nov 10

#teamMassSpec here is a starting pack in case you just moved over, or in case you've been absent for a while. This is #proteomics or #massspec related. The pack is not comprehensive but it's a start.

go.bsky.app/HH7kqEh

19 51 89