van der Net et al. show that mechanical interactions with the stromal component collagen I trigger reprogramming of colorectal cancer cells into a fetal-like state, through mechanosensitive integrins ...

Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01252-7CRISPR screens are widely used to identify essential genes and potential drug targets, but typical setups were designed for the study of highly proliferative cancer cell lines. Now, a protocol, using inducible Cas9, enables screens of non-proliferative cell states including senescence, quiescence and terminal differentiation, thereby expanding the applicability of CRISPR screens.

Hyperactivation of WNT signaling is a well-established hallmark of cancer. Various epithelial cancers express high levels of WNT7B and WNT10A that are not commonly expressed during tissue homeostasis,...