Nature Protocols
@natprot.nature.com
New Article! Putting mammalian early embryonic cells into dormancy
Putting mammalian early embryonic cells into dormancy
Nature Protocols, Published online: 26 January 2026; doi:10.1038/s41596-025-01303-zThe authors provide a suite of in vitro protocols through which mouse and human pluripotent stem cells, mouse blastocysts and human blastoids can be reversibly induced to enter a diapause-like dormant state via pharmacological inhibition of mTOR
dlvr.it
January 27, 2026 at 12:12 AM
New Article! Putting mammalian early embryonic cells into dormancy
New Article! Investigation of mechanical forces during multicellular early angiogenic sprouting by three-dimensional traction force microscopy in hydrogel matrices
Investigation of mechanical forces during multicellular early angiogenic sprouting by three-dimensional traction force microscopy in hydrogel matrices
Nature Protocols, Published online: 23 January 2026; doi:10.1038/s41596-025-01275-0Investigating mechanical forces during angiogenic sprouting by three-dimensional traction force microscopy (TFM) in hydrogel matrices, including steps to analyze and interpret cell–extracellular matrix forces using open-source TFMLAB software.
dlvr.it
January 23, 2026 at 10:20 AM
New Article! Investigation of mechanical forces during multicellular early angiogenic sprouting by three-dimensional traction force microscopy in hydrogel matrices
New Article! Screening high-quality lignins extracted from biomass to prepare biobased wood adhesives
Screening high-quality lignins extracted from biomass to prepare biobased wood adhesives
Nature Protocols, Published online: 22 January 2026; doi:10.1038/s41596-025-01316-8This protocol isolates lignin from various types of biomass, screens for high-quality lignins, and uses them to directly prepare lignin adhesives without chemical modification, the performance of which is then assessed. It produces lignin adhesives with light colors and superior properties.
dlvr.it
January 23, 2026 at 3:56 AM
New Article! Screening high-quality lignins extracted from biomass to prepare biobased wood adhesives
New Article! Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas
Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas
Nature Protocols, Published online: 21 January 2026; doi:10.1038/s41596-025-01287-wThis protocol details transsynaptic tracing approaches using retrograde and anterograde viral tracers to map and manipulate neuron–tumor circuits in xenografts, brain organoid models and co-cultures.
dlvr.it
January 21, 2026 at 3:01 PM
New Article! Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas
New Article! Electrocatalytic C‒N bond construction from inorganic nitrogen sources in water
Electrocatalytic C‒N bond construction from inorganic nitrogen sources in water
Nature Protocols, Published online: 20 January 2026; doi:10.1038/s41596-025-01298-7Urea, formamide, cyclohexanone oxime and amino acids are vital raw materials. Their synthesis often requires high temperatures and dangerous reagents. This protocol describes an aqueous electrocatalytic approach using inorganic nitrogen sources.
dlvr.it
January 20, 2026 at 12:58 PM
New Article! Electrocatalytic C‒N bond construction from inorganic nitrogen sources in water
New Article! Standardized protocols for analyzing lipocartilage and lipochondrocytes
Standardized protocols for analyzing lipocartilage and lipochondrocytes
Nature Protocols, Published online: 19 January 2026; doi:10.1038/s41596-025-01324-8Lipocartilage has been identified as a novel adipose-related cartilage subtype generated by lipochondrocytes, which produce intracellular lipid vacuoles that provide structural rigidity to the outer ear. Now, a protocol for reproducible isolation of lipocartilage and lipochondrocytes in mice is reported.
dlvr.it
January 20, 2026 at 2:28 AM
New Article! Standardized protocols for analyzing lipocartilage and lipochondrocytes
New Article! A comprehensive framework for statistical testing of brain dynamics
A comprehensive framework for statistical testing of brain dynamics
Nature Protocols, Published online: 19 January 2026; doi:10.1038/s41596-025-01300-2This protocol covers a package for running statistical testing on the temporal dynamics of neural activity data obtained from various functional neural recording modalities.
dlvr.it
January 20, 2026 at 2:28 AM
New Article! A comprehensive framework for statistical testing of brain dynamics
New Article! GPSeq maps the radial organization of eukaryotic genomes along the nuclear periphery–center axis
GPSeq maps the radial organization of eukaryotic genomes along the nuclear periphery–center axis
Nature Protocols, Published online: 19 January 2026; doi:10.1038/s41596-025-01278-xGPSeq is a genome-wide method for probing the radial organization of the genome in cells or nuclei by progressive in situ digestion of chromatin with a restriction enzyme diffusing inward from the periphery followed by high-throughput sequencing.
dlvr.it
January 20, 2026 at 2:28 AM
New Article! GPSeq maps the radial organization of eukaryotic genomes along the nuclear periphery–center axis
New Article! Isolation, purification and characterization of lipocartilage in mice
Isolation, purification and characterization of lipocartilage in mice
Nature Protocols, Published online: 19 January 2026; doi:10.1038/s41596-025-01302-0Here Plikus and colleagues provide a step-by-step protocol for the isolation and characterization of lipocartilage from mouse ear and the purification of its lipochondrocytes.
dlvr.it
January 20, 2026 at 2:28 AM
New Article! Isolation, purification and characterization of lipocartilage in mice
Join us on 22 January 2026 for “Methods Mythbusting: Insights from Journal Editors and protocols.xn--io-02t
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January 19, 2026 at 11:05 AM
Join us on 22 January 2026 for “Methods Mythbusting: Insights from Journal Editors and protocols.xn--io-02t
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Reposted by Nature Protocols
We’re thrilled to share our latest iTP-seq protocol for mapping bacterial translation landscapes in vitro — with a complete experimental workflow streamlined by Mélanie Gillard and an open source Python library for data analysis developed by Thibaud Renault.
January 17, 2026 at 2:22 PM
We’re thrilled to share our latest iTP-seq protocol for mapping bacterial translation landscapes in vitro — with a complete experimental workflow streamlined by Mélanie Gillard and an open source Python library for data analysis developed by Thibaud Renault.
New Article! Profiling active RNA polymerase II transcription start sites from total RNA by capped small RNA sequencing (csRNA-seq)
Profiling active RNA polymerase II transcription start sites from total RNA by capped small RNA sequencing (csRNA-seq)
Nature Protocols, Published online: 16 January 2026; doi:10.1038/s41596-025-01285-yWe present a protocol for high-resolution genome-wide mapping of nascent RNA polymerase II transcription initiation of both stable RNAs and transiently expressed RNAs using total RNA from diverse sample types.
dlvr.it
January 16, 2026 at 9:23 AM
New Article! Profiling active RNA polymerase II transcription start sites from total RNA by capped small RNA sequencing (csRNA-seq)
Reposted by Nature Protocols
💡 Did you know? Methods are among the most cited research outputs.
For #LoveMethods26, join experts for interactive sessions:
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January 15, 2026 at 2:21 PM
💡 Did you know? Methods are among the most cited research outputs.
For #LoveMethods26, join experts for interactive sessions:
👉 Jan 21: @protocolsio.bsky.social Hackathon
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For #LoveMethods26, join experts for interactive sessions:
👉 Jan 21: @protocolsio.bsky.social Hackathon
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New Article! iTP-seq: a scalable profiling workflow to characterize bacterial translation landscapes in vitro
iTP-seq: a scalable profiling workflow to characterize bacterial translation landscapes in vitro
Nature Protocols, Published online: 15 January 2026; doi:10.1038/s41596-025-01294-xThis protocol describes inverse toeprinting coupled to next-generation sequencing, an in vitro approach to characterize bacterial translation at codon resolution that can accommodate custom synthetic libraries and various translation perturbations.
dlvr.it
January 15, 2026 at 10:22 AM
New Article! iTP-seq: a scalable profiling workflow to characterize bacterial translation landscapes in vitro
New Article! Upconversion particle-based optical tweezers for sensing applications
Upconversion particle-based optical tweezers for sensing applications
Nature Protocols, Published online: 15 January 2026; doi:10.1038/s41596-025-01264-3Upconversion particle-based holographic fluorescence optical tweezers enable super-resolved photonic force microscopy and applications on long-range subfemtonewton force sensing, intracellular viscosity measurements and temperature sensing.
dlvr.it
January 15, 2026 at 10:22 AM
New Article! Upconversion particle-based optical tweezers for sensing applications
I edit manuscripts on very diverse topics and frequently have to look up words that are new to me.
Today the word was "rugosity".
A few weeks ago it was "psychrophilia".
The question is: Can I assume that the interested reader will know what they mean?
Today the word was "rugosity".
A few weeks ago it was "psychrophilia".
The question is: Can I assume that the interested reader will know what they mean?
January 15, 2026 at 9:40 AM
I edit manuscripts on very diverse topics and frequently have to look up words that are new to me.
Today the word was "rugosity".
A few weeks ago it was "psychrophilia".
The question is: Can I assume that the interested reader will know what they mean?
Today the word was "rugosity".
A few weeks ago it was "psychrophilia".
The question is: Can I assume that the interested reader will know what they mean?
New Article! Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq
Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq
Nature Protocols, Published online: 12 January 2026; doi:10.1038/s41596-025-01304-yThis protocol describes a flexible workflow for joint profiling of chromatin accessibility and transcriptomes in single nuclei, compatible with either plate or droplet single-cell isolation platforms.
dlvr.it
January 12, 2026 at 10:48 AM
New Article! Single-nucleus chromatin accessibility and gene expression co-profiling by ISSAAC-seq
New Article! Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level
Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level
Nature Protocols, Published online: 12 January 2026; doi:10.1038/s41596-025-01279-wA strategy for isolating Escherichia coli ribosome–nascent chain complexes and analyzing their conformational dynamics and interactors at peptide level using hydrogen–deuterium exchange mass spectrometry is presented.
dlvr.it
January 12, 2026 at 10:48 AM
New Article! Hydrogen/deuterium exchange mass spectrometry analysis of ribosome-nascent chain complexes to study protein biogenesis at the peptide level
New Article! Deployment of a cloud-based passive defecation monitoring system for continuous gut health monitoring
Deployment of a cloud-based passive defecation monitoring system for continuous gut health monitoring
Nature Protocols, Published online: 09 January 2026; doi:10.1038/s41596-025-01296-9In this protocol, the authors explain how to set up and use the cloud-based Precision Health Integrated Diagnostic (PHIND) system, a smart-toilet-based platform for passive and automated defecation monitoring to provide continuous gut health information.
dlvr.it
January 10, 2026 at 9:30 AM
New Article! Deployment of a cloud-based passive defecation monitoring system for continuous gut health monitoring
New Article! Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles
Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles
Nature Protocols, Published online: 09 January 2026; doi:10.1038/s41596-025-01286-xThis protocol covers the acoustics-based separation of biological particles such as viruses and single extracellular vesicles from biofluids, including plasma and saliva, with high purity.
dlvr.it
January 10, 2026 at 9:30 AM
New Article! Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles
New Article! In vivo Raman spectroscopy for real-time biochemical assessment of tissue pathology and physiology
In vivo Raman spectroscopy for real-time biochemical assessment of tissue pathology and physiology
Nature Protocols, Published online: 07 January 2026; doi:10.1038/s41596-025-01274-1We present a protocol for the acquisition and analysis of in vivo Raman spectra to extract biochemical information from tissues for biomedical applications.
bit.ly
January 7, 2026 at 9:04 AM
New Article! In vivo Raman spectroscopy for real-time biochemical assessment of tissue pathology and physiology
Take a look at Nature Methods December issue for 8 methods to watch in 2026! Virtual cells, the cancer microbiome, protein editing, and more... www.nature.com/nmeth/volume...
Nature Methods - Method of the Year 2025: EM-based connectomics
Auditory neurons in the FlyWire/FAFB whole-brain connectome.See Editorial
www.nature.com
January 6, 2026 at 6:53 PM
Take a look at Nature Methods December issue for 8 methods to watch in 2026! Virtual cells, the cancer microbiome, protein editing, and more... www.nature.com/nmeth/volume...
Reposted by Nature Protocols
Our Method of the Year 2025 is...drumroll please...EM-based connectomics!!
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Method of the Year 2025: electron microscopy-based connectomics - Nature Methods
A large network of interconnected neurons serves as the basis of brain function and of behavior. Methodological advances have enabled the reconstruction of large-scale and even whole-brain connectomes...
www.nature.com
December 8, 2025 at 4:59 PM
Our Method of the Year 2025 is...drumroll please...EM-based connectomics!!
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Our Editorial introduces our choice and highlights six Comments and other related content in this special issue. Please join us in celebrating EM-based connectomics! 🎉🧠🔬
www.nature.com/articles/s41...
Join Springer Nature editors and the protocols.io team for two exciting events at #LoveMethods26!
💻Transform your protocols at our Hackathon - Jan 21
🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22
👉FREE registration & more details: excelscior.uc.pt/love-methods...
💻Transform your protocols at our Hackathon - Jan 21
🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22
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Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
protocols.io
January 6, 2026 at 2:11 PM
Join Springer Nature editors and the protocols.io team for two exciting events at #LoveMethods26!
💻Transform your protocols at our Hackathon - Jan 21
🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22
👉FREE registration & more details: excelscior.uc.pt/love-methods...
💻Transform your protocols at our Hackathon - Jan 21
🔮Gain insight on methods publishing at our Methods Mythbusting - Jan 22
👉FREE registration & more details: excelscior.uc.pt/love-methods...
New Article! CRISPR screens in human neural organoids and assembloids
CRISPR screens in human neural organoids and assembloids
Nature Protocols, Published online: 19 December 2025; doi:10.1038/s41596-025-01299-6This protocol combines pooled CRISPR–Cas9 screening with neural organoid and assembloid models and illustrates how it can be applied to map hundreds of disease genes onto cellular pathways and specific aspects of human neural development.
bit.ly
December 19, 2025 at 9:18 PM
New Article! CRISPR screens in human neural organoids and assembloids