BaglaenkoLab
@baglaenkolab.bsky.social
The Baglaenko Lab of Immunogenetics in the Division of Human Genetics at Cincinnati Children's Hospital is focused on developing new experimental and computational approaches to answer the most difficult questions in inflammatory and infectious diseases.
Reposted by BaglaenkoLab
Nature research paper: Precisely defining disease variant effects in CRISPR-edited single cells
go.nature.com/44M3ygX
go.nature.com/44M3ygX
Precisely defining disease variant effects in CRISPR-edited single cells - Nature
A plate-based assay called CRAFTseq has been developed that uses ‘multi-omic’ single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants on cell state and function.
go.nature.com
July 29, 2025 at 6:38 PM
Nature research paper: Precisely defining disease variant effects in CRISPR-edited single cells
go.nature.com/44M3ygX
go.nature.com/44M3ygX
I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Precisely defining disease variant effects in CRISPR-edited single cells - Nature
A plate-based assay called CRAFTseq has been developed that uses ‘multi-omic’ single-cell RNA sequencing and direct genotyping of CRISPR edits to test the functional effects of genetic variants o...
www.nature.com
July 23, 2025 at 3:21 PM
I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
My lab thinks a lot about CRISPR editing variants of interest in primary human cells. We made a little tool to help. Hopefully, someone else can get some use out of it. baglaenkolab.shinyapps.io/snpGuide/
github.com/ybaglaenko/s...
github.com/ybaglaenko/s...
shiny.io
June 2, 2025 at 8:19 PM
My lab thinks a lot about CRISPR editing variants of interest in primary human cells. We made a little tool to help. Hopefully, someone else can get some use out of it. baglaenkolab.shinyapps.io/snpGuide/
github.com/ybaglaenko/s...
github.com/ybaglaenko/s...