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Rachael Ott

@rachaelott.bsky.social

2.4K followers 2.4K following 160 posts

Neurobiologist @UofSC 🔬 Super-resolution #microscopy, synaptic plasticity, cytoskeleton, #neurodegeneration 🦠

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Rachael Ott @rachaelott.bsky.social · Jan 17

For #FluorescenceFriday, tau (blue) and long loops of neurofilament proteins are shown in a primary hippocampal neuron 🔬 #Neuroscience #Microscopy

Tau (blue) and neurofilament (fire) are shown in a primary hippocampal neuron.

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Reposted by Rachael Ott

Kai Murk @kaimurk.bsky.social · 16h

This #microscopymonday is a cleaning day. Watch how a #microglia cell mops up and phagozytoses debris and junk in a very messy neuronal co-culture. BTW, it was a proper cleaning job → the neurons made it and formed nice networks
Phase contrast imaging started at DIV1. One frame per 20 min.

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Reposted by Rachael Ott

adesmazieres.bsky.social @adesmazieres.bsky.social · Nov 15

Join the Glia community on bluesky go.bsky.app/Lyzro3M

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Rachael Ott @rachaelott.bsky.social · 1d

Gorgeous clip!

Reposted by Rachael Ott

Dylan Burnette @mag2art.bsky.social · 1d

Fish keratocytes isolated from a fish scale crawling around. Technique: DIC microscopy. #CellBiology.
Another #NikonSmallWorldReject

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Rachael Ott @rachaelott.bsky.social · 1d

Thank you Catalina!

Reposted by Rachael Ott

Rachael Ott @rachaelott.bsky.social · 2d

For #FluorescenceFriday, α-spectrin (red) and lipid droplets (green) are shown in adipocytes 🔬 #Microscopy #CellBiology

α-spectrin (red), lipid droplets (green), and nuclei (blue) are shown in adipocytes. Scale bar is 20 μm.

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Rachael Ott @rachaelott.bsky.social · 2d

Protocol is linked here :)

Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adult Drosophila melanogaster adipose tissue

Summary: Optimization of adult Drosophila fat body fluorescence microscopy via modifications of tissue handling, fixation, and permeabilization steps in a whole-mount immunolabeling protocol.

journals.biologists.com

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Rachael Ott @rachaelott.bsky.social · 2d

Thank you! It’s BODIPY 505/510

Rachael Ott @rachaelott.bsky.social · 2d

For #FluorescenceFriday, α-spectrin (red) and lipid droplets (green) are shown in adipocytes 🔬 #Microscopy #CellBiology

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Reposted by Rachael Ott

Danielle Beckman @daniellebeckman.bsky.social · 2d

Viral trigger in Alzheimer’s disease is the real scary spooky #FluorescenceFriday.
Amyloid-β🟣 is an antimicrobial peptide that can be produced to trap pathogens in the brain. But it also induces an extensive neuroinflammatory response, as shown here with 🟠astrocytes. #NeuroCovid

Confocal microscopy photo showing astrocytes and amyloid-B

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Reposted by Rachael Ott

Hao Yin @haoyin.bsky.social · 2d

Sometimes, poor perfusion is attributed to a Venule🤓

Age-related rarefaction of capillary meshwork converging into principal cortical venules, most pronounced in layer 6/CC➡️
Gliosis+demyelination in 🐭 #WhiteMatterAging

#SciArt
@andyshihlab.bsky.social #NatNeuro 2025
www.nature.com/articles/s41...

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Reposted by Rachael Ott

Andrea Caldarola @andreacaldarola.bsky.social · 2d

For #FluorescenceFriday : 3 cells start at the same Ca2+ concentration but undergo 3 different Histamine responses. Imaged using the lifetime-based sensor Tq-Ca-FLITS.

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Reposted by Rachael Ott

Christophe 🔬 L @christlet.bsky.social · 17d

Beautiful live-cell imaging of lysosome tubulation along microtubules in this work just out from @lysoluis.bsky.social et al:
rupress.org/jcb/article/...

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Reposted by Rachael Ott

Schallek Lab @schalleklab.bsky.social · 3d

#FluorescenceFriday
Watch to the end! Immune cells in the retina remain flat until damage is detected. Cells change their shape and dive deep in response to damage (pink cell)
@cvsuor.bsky.social @urochester.bsky.social
@flaumeye.bsky.social @uofrbme.bsky.social
elifesciences.org/articles/98662

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Reposted by Rachael Ott

Stacey Ogden 🦔 @labogden.bsky.social · 2d

Happy #FluorescenceFriday! This is a maximum intensity projection of depth shaded actin (🌈) in a section from an E9.5 🐭 neural tube (NT). 🔬 by postdoc @christinaadaly.bsky.social 👩‍🔬 🧪 Image shows the floor plate and lumen of the developing NT.

#SciArt #DevBio #DevNeuro

Rainbow shading of a confocal micrograph of a developing mouse neural tube. The protein actin is stained and colored in rainbow to indicate depth in the section.

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Reposted by Rachael Ott

Nat Clarke @natclarke.bsky.social · 4d

🚨 My lab is hiring at all levels!

Interested in animal origins & evolutionary cell biology?

I'm recruiting a postdoc, PhD students & a research assistant to study the molecular evolution of cell adhesion using marine invertebrates + comparative genomics.

🔗: clarkelab.com/join/

Please repost!

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Reposted by Rachael Ott

Journal of Cell Biology @jcb.org · Aug 11

Geng, @kjverhey1.bsky.social et al find that KIF1C forms condensates where the exposed #kinesin motor domains entangle nearby #microtubules, causing them to bend & break in a process dependent on motor processivity, cluster properties, cytoplasmic viscosity, & MT anchors rupress.org/jcb/article/...

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Reposted by Rachael Ott

Manuel Thery @manuelthery.bsky.social · 5d

What would happen if cells would express more molecular motors ?
They would break all microtubules 😱
As shown by recent great work from @kjverhey1.bsky.social

rupress.org/jcb/article-...

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Reposted by Rachael Ott

Reto Fiolka @retof.bsky.social · 6d

We present multi-immersion Oblique Plane microscope (miOPM), a light-sheet platform that can be adapted to a wide range of applications, from sensitive live cell imaging to imaging organs and cleared tissues.
www.biorxiv.org/content/10.1...

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Reposted by Rachael Ott

Shweta Nandakumar @bucklingmt.bsky.social · 6d

Playing around with @kwolbachia.bsky.social 's LUT manager plugin. Absolutely love the campfire LUT🔥 😍#microtubulemonday

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Reposted by Rachael Ott

Ed Hutchinson @socialinfluenza.bsky.social · 6d

This is incredibly beautiful, and the fact that the resolution is sufficient to follow the path of DNA spooling inside the virion head and through the tail is amazing

The Daum Lab @daumlab.bsky.social · 6d

Out in Science Advances: Our #cryoEM structure of HFTV1, a virus infecting the halophile #archaea. *First full atomic structure (containing all structural proteins) of any tailed virus!* Congrats and thanks to all co-authors and our fantastic collaborators! www.science.org/doi/10.1126/...

Cryo-EM resolves the structure of the archaeal dsDNA virus HFTV1 from head to tail

This structure of an archaeal tailed virus (arTV) provides detailed insights into arTV assembly and infection mechanisms.

www.science.org

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Rachael Ott @rachaelott.bsky.social · 7d

Thank you Anna!

Reposted by Rachael Ott

Rachael Ott @rachaelott.bsky.social · 9d

For #FluorescenceFriday, RhoA (blue) and the actin cytoskeleton (magenta) are shown in a set of primary microglia 🔬 #Neuroscience #Microscopy

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Rachael Ott @rachaelott.bsky.social · 8d

Thank you Shweta! 😄

Reposted by Rachael Ott

Lori O'Brien @llobrienlab.bsky.social · 9d

Sharing this excellent review now out from postdoc Sarah McLarnon! Lots of useful info on 3D imaging, quantification of imaging data, and how this has been applied to the kidney. And since it's #FluorescenceFriday, sharing a figure with our own imaging data!
www.sciencedirect.com/science/arti...

Figure from our review paper showing various fluorescence microscopy images of cells and structures of the kidney

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