#snRNAseq
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jia-shin1022.bsky.social
At the single-cell level, APOE4×Aβ showed a clear synergistic effect in neurons.
Both excitatory and inhibitory populations displayed a distinct interaction signature not seen with either genotype alone.
Early synergistic APOE4xAβ interaction emerges predominantly at the transcriptomic level in neurons, but not in glial cells. Hippocampi from aged (14–15-month-old) WT, E4, NLF, and E4NLF littermate mice (n = 7–8 mice per genotype) were dissected and processed for snRNAseq (n = 133,413 nuclei) and canonically clustered into interneurons, pyramidal cells, microglia, and astrocytes. (a) Pseudo-bulk weighted gene co-expression network analysis (WGCNA) showing module eigengene (ME) relationships across interneurons, pyramidal cells, microglia, and astrocytes. Module eigengene heatmaps show genotype-dependent relationships for each major hippocampal cell type.
November 16, 2025 at 10:35 PM
alexkwan.bsky.social
Lab is thrilled to leave frigid NY to go to sunny San Diego for #SfN2025 😂

Mon AM posters:
LBP182 - Psilocybin and layer 1 interneurons
LBP183 - Psilocybin in chronic stress model
LBP184 - Drugs + snRNAseq

Tue PM posters:
LL18 - Psilocybin and pyramidal cell types
MM3 - Drugs + rabies tracing
November 13, 2025 at 9:33 PM
lcolladotor.bsky.social
Para refinar la señal de habénula del vecino tálamo utilizamos nuestros datos de #snRNAseq para #deconvolución de nuestras muestras a granel #bulkRNA, estimando así la proporción de Hb. Estas proporciones las usamos para controlar diferencias en disecciones en el análisis de expresión diferencial 📊
November 5, 2025 at 11:47 PM
lcolladotor.bsky.social
Para contextualizar, integramos nuestros datos de #snRNAseq de Hb humana con datos #singlecell previamente publicados de Hb de ratón por el laboratorio de @gstuber.bsky.social. Algunos grupos de células neuronales como las LHb.2 enriquecidas por OPRM1 en humano aparecen conservadas entre especies 🐁
November 5, 2025 at 11:47 PM
lcolladotor.bsky.social
Creamos un mapa molecular con #snRNAseq de la Hb humana para entender la diversidad neuronal a un nivel molecular en esta región del cerebro única. Nuestros datos de 7 donadores control produjeron 16 mil núcleos y 17 grupos celulares, incluyendo 7 poblaciones neuronales laterales y 3 medias en Hb 🧪
November 5, 2025 at 11:47 PM
lcolladotor.bsky.social
Acá va en español 🇲🇽
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"¡Estamos orgullosos de anunciar que nuestro artículo ‘Análisis Transcriptómico de la Habénula Humana en Esquizofrenia’ es el artículo de portada de la edición de noviembre del American Journal of Psychiatry!" 🧠 #HabenulaLIBD #snRNAseq #Habénula doi.org/10.1176/appi...
November 5, 2025 at 11:47 PM
lahuuki.bsky.social
To refine habenula signal from neighboring thalamus we utilized our #snRNAseq data to perform #deconvolution on our bulk samples, estimating proportion of habenula. These proportions were used to control for dissection differences in the differential expression analysis
November 4, 2025 at 9:31 PM
lahuuki.bsky.social
We’ve created a #snRNAseq molecular atlas of the human Habenula to understand neuronal diversity on a molecular level in this unique brain region. Our snRNA-seq data on 7 control donors yielded 16k nuclei and 17 clusters, including 7 lateral & 3 medial Hb neuron populations 🧪
November 4, 2025 at 9:31 PM
lahuuki.bsky.social
Proud to announce our paper ‘Transcriptomic Analysis of the Human Habenula in Schizophrenia’ from @lieberinstitute.bsky.social is the cover article for the November issue of the American Journal of Psychiatry! 🧠 #HabenulaLIBD #snRNAseq #Habenula doi.org/10.1176/appi...
Transcriptomic Analysis of the Human Habenula in Schizophrenia | American Journal of Psychiatry
Objective: The objective of this study was to define the molecular neuroanatomy of the human habenula (Hb) and identify transcriptomic differences between brains of individuals with schizophrenia and ...
doi.org
November 4, 2025 at 9:31 PM
dev-journal.bsky.social
A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex

Read this this #LifelongDevSI #OA Techniques and Resources Article by Andre M. Xavier, Qianyu Lin, Chris J. Kang, Lucas Cheadle (@cheadlelab.bsky.social)

doi.org/10.1242/dev....
Figure 1 - Experimental design and introduction to the single-nucleus RNA sequencing dataset. (A) Schematic illustrating the late dark-rearing (LDR) paradigm and the workflow of the single-nucleus RNA sequencing (snRNAseq) experiments. (B) Quantification of Fos mRNA expression in sensory deprived (LDR) mice and in mice acutely exposed to light for between 15 min and 2 h, with stimulation timepoints labeled as follows: LDR15m (15 min of light), LDR30m (30 min), LDR1h (1 h) and LDR2h (2 h). Fos expression assessed by qPCR and normalized to Gapdh. Values plotted are additionally normalized to the LDR condition. (C) qPCR quantification of Jun mRNA expression (normalized to Gapdh) in V1 across all timepoints. Data obtained by qPCR and values plotted are normalized to LDR. In B and C, data are mean±s.e.m. n=3 mice per condition. One-way ANOVA followed by Tukey's post hoc test: *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. (D) Example confocal images of V1 in sections from a sensory deprived mouse (LDR) and a mouse re-exposed to light for 30 min (LDR30m). Fos mRNA (red), Jun mRNA (green) and DAPI (blue). Scale bar: 44 µm. (E) UMAP plot illustrating the 118,529 nuclei in the dataset categorized by general cell class: excitatory neurons (blue), inhibitory neurons (pink) and glia (green). (F) UMAP plot with all 16 clusters colored and labeled by cell type.
November 3, 2025 at 3:59 PM
erinhillburns.bsky.social
Opposite directions of association of higher physical activity and higher insulin resistance with human skeletal muscle cell type abundance and fiber type-level gene expression #snRNAseq #SingleNucleus #RNAseq #genomics 🧪🧬🖥️ www.biorxiv.org/content/10.1...
www.biorxiv.org
October 31, 2025 at 11:06 AM
precisioncellsys.bsky.social
Read the study: precisioncellsystems.com/resources/?_...

#SingleCell #snRNAseq #Automation #SamplePrep #Neuroscience #PrecisionCellSystems
Resource Library - Precision Cell Systems
Browse our collection of published protocols, peer-reviewed publications, and application notes featuring Precision Cell Systems technology.
precisioncellsystems.com
October 30, 2025 at 6:23 PM
mori-lab.bsky.social
We observed the same phenomenon in several other cancer types, such as CRC, prostate and breast. Specifically, it is a 10-year prospective cohort snRNAseq study of PDAC patients with initial metastatic recurrence in either liver or lung:
October 27, 2025 at 12:14 PM
kaessmannlab.bsky.social
In this study, led by @Xuefei and @Leti, we generated snRNAseq & snATACseq data from the livers of 17 species, covering:
a. Different diets (herbivores, omnivores, carnivores, insectivores) 🌿¬🍖🐜
b. Main mammalian lineages (monotremes, marsupials, placentals)🐒🐈🐑🦘
c. A bird outgroup 🐔
October 15, 2025 at 12:51 PM
prashanthi-ravi.bsky.social
(1/10) We’re excited to share our new preprint (doi.org/10.1101/2025...), which uncovers the spatiomolecular landscape of the human nucleus accumbens (NAc), by integrating snRNA-seq with Visium spatial transcriptomics across 10 control donors. 🧠 #NAcLIBD #snRNAseq #10xVisium
September 15, 2025 at 5:06 PM
ccstorytime.bsky.social
At the @dsmb-dk.bsky.social Annual Meeting 2025 and PhD student Ask Møbjerg is presenting our latest work on the acute effects of exercise on ECM remodelling of human muscle-tendon tissues using snRNAseq and RNAscope 🏋️‍♀️🔬

@ismcopenhagen.bsky.social
September 9, 2025 at 9:48 AM
abigailmackey1.bsky.social
Check out this cool preprint led by @ccstorytime.bsky.social with snRNAseq data on 60,000 nuclei from human muscle-tendon tissue 4 h after exercise

@myoblue.bsky.social
ccstorytime.bsky.social Chloé Yeung @ccstorytime.bsky.social · Sep 2
BSMB Autumn meeting Surrey talking about our latest work. Very proud of Team Tendon & collaborators for persevering with such a difficult tissue 👏
www.biorxiv.org/content/10.1...
@ismcopenhagen.bsky.social @steffendanielle.bsky.social @valerioizzi.bsky.social @abigailmackey1.bsky.social
September 3, 2025 at 4:40 AM
prepub-neurodegen.bsky.social
Comparative snRNAseq study of C9orf72, SOD1, and sALS spinal cord #NeuroDegeneration 🧪🧠
https://www.biorxiv.org/content/10.1101/2025.08.26.672029v1
August 30, 2025 at 4:01 PM
biorxivpreprint.bsky.social
Comparative snRNAseq study of C9orf72, SOD1, and sALS spinal cord https://www.biorxiv.org/content/10.1101/2025.08.26.672029v1
August 30, 2025 at 8:15 AM
biorxiv-neursci.bsky.social
Comparative snRNAseq study of C9orf72, SOD1, and sALS spinal cord https://www.biorxiv.org/content/10.1101/2025.08.26.672029v1
August 30, 2025 at 8:15 AM
flybottleescape.bsky.social
5. A functional ensemble is not just one molecular cell-type right? Gene markers are proposed for (-)valence—Rspo2 + Fezf2

Using snRNAseq we mapped noxious immediate early genes (IEGs) to find two subtypes of Rspo2+ neurons not expressing Fezf2 or other “valence markers”
July 24, 2025 at 1:55 PM
cronchi.bsky.social
Highlights from an exciting Day 2 at @endocrinesociety.bsky.social #ENDO2025 including my talk on #snRNAseq in #ACC at the #adrenalcancer symposium! @unibirmingham.bsky.social @uhbtrust.bsky.social
July 14, 2025 at 5:35 AM
dpalcon.bsky.social
El TFM es sobre el Análisis y validación funcional de genes y elementos cis reguladores (enhancers) involucrados en la especificación de territorios de la copa óptica del pez cebra, identificados mediante snRNAseq y snATACseq
July 9, 2025 at 4:07 PM
haoyin.bsky.social
#SpatialTranscriptomics #ParkinsonsDisease

A role of #FGF9 in Glia #Ferroptosis in PD?😁

+snRNAseq
MPTP🐭 #SubstantiaNigra

FGF9 ⏬in PD Neuron ⏫in PD Ependymal cell

FGF9-to-FGFR1/2/3 (CellChat, likely Neuron-to-Oligodendrocyte/Astrocyte) ⏬in PD

#NPJParkinsonsDis 2025
www.nature.com/articles/s41...
July 2, 2025 at 12:08 PM
rgottardo.bsky.social
All data are available including manually annotated single-cells (snRNAseq) and Visium spots! Great for benchmarking algorithms and computational tools!
June 22, 2025 at 7:09 AM