🐣 Jesse Thomas from @csiro.bsky.social & @deakinuniversity.bsky.social gave us a highlight of the design of improved diagnostic qPCR assays for IBDV detection, an economically important poultry disease in Australia
#YoungVIIN2025 #VIINYIS
#YoungVIIN2025 #VIINYIS
November 14, 2025 at 3:58 AM
🐣 Jesse Thomas from @csiro.bsky.social & @deakinuniversity.bsky.social gave us a highlight of the design of improved diagnostic qPCR assays for IBDV detection, an economically important poultry disease in Australia
#YoungVIIN2025 #VIINYIS
#YoungVIIN2025 #VIINYIS
A new reporter mouse for visualising Hedgehog/GLI signalling activity
This Research Highlight showcases the work from Hannah Schrader Dear, Nicole Franks, Benjamin Allen and colleagues:
journals.biologists.com/dev/article/...
This Research Highlight showcases the work from Hannah Schrader Dear, Nicole Franks, Benjamin Allen and colleagues:
journals.biologists.com/dev/article/...
November 7, 2025 at 12:33 PM
A new reporter mouse for visualising Hedgehog/GLI signalling activity
This Research Highlight showcases the work from Hannah Schrader Dear, Nicole Franks, Benjamin Allen and colleagues:
journals.biologists.com/dev/article/...
This Research Highlight showcases the work from Hannah Schrader Dear, Nicole Franks, Benjamin Allen and colleagues:
journals.biologists.com/dev/article/...
From #PCR to #NGS library prep, the right #DNApolymerase makes all the difference. Watchmaker offers a full portfolio for any workflow, from robust PCR/qPCR & isothermal amplification to high-fidelity NGS and specialty enzymes.
👉 Explore the portfolio: t.co/IIHaazI4A5
👉 Explore the portfolio: t.co/IIHaazI4A5
October 28, 2025 at 3:03 PM
From #PCR to #NGS library prep, the right #DNApolymerase makes all the difference. Watchmaker offers a full portfolio for any workflow, from robust PCR/qPCR & isothermal amplification to high-fidelity NGS and specialty enzymes.
👉 Explore the portfolio: t.co/IIHaazI4A5
👉 Explore the portfolio: t.co/IIHaazI4A5
Our qPCR data is now protected by sharks!
October 23, 2025 at 9:02 PM
Our qPCR data is now protected by sharks!
Using RT-qPCR as the reference standard on 197 nasopharyngeal swabs,
the Pluslife assay showed a positive percentage agreement (PPA) of 96.30% and a
negative percentage agreement (NPA) of 99.30% (κ=0.962, P<0.001). The EasyNAT
assay demonstrated a PPA of 96.30% and NPA of 98.60% (κ=0.949, P<0.001).
the Pluslife assay showed a positive percentage agreement (PPA) of 96.30% and a
negative percentage agreement (NPA) of 99.30% (κ=0.962, P<0.001). The EasyNAT
assay demonstrated a PPA of 96.30% and NPA of 98.60% (κ=0.949, P<0.001).
October 24, 2025 at 7:15 AM
Using RT-qPCR as the reference standard on 197 nasopharyngeal swabs,
the Pluslife assay showed a positive percentage agreement (PPA) of 96.30% and a
negative percentage agreement (NPA) of 99.30% (κ=0.962, P<0.001). The EasyNAT
assay demonstrated a PPA of 96.30% and NPA of 98.60% (κ=0.949, P<0.001).
the Pluslife assay showed a positive percentage agreement (PPA) of 96.30% and a
negative percentage agreement (NPA) of 99.30% (κ=0.962, P<0.001). The EasyNAT
assay demonstrated a PPA of 96.30% and NPA of 98.60% (κ=0.949, P<0.001).
Azenta-Genewiz - currently wiping the floor with Sigma, IDT and especially ThermoFisher for qPCR dual labelled probes. £85 versus £350 (Genewiz vs Thermo). It pays to shop around...
October 24, 2025 at 10:30 AM
Azenta-Genewiz - currently wiping the floor with Sigma, IDT and especially ThermoFisher for qPCR dual labelled probes. £85 versus £350 (Genewiz vs Thermo). It pays to shop around...
Entweder qPCR für spezifische Fragen oder Next generation RNA-Sequenzierung für das Metagenom 😉
October 14, 2025 at 10:22 AM
Entweder qPCR für spezifische Fragen oder Next generation RNA-Sequenzierung für das Metagenom 😉
In Romania (2024), backyard birds—chickens, geese, ducks, pheasants—did not sustain circulation. An H5N1 outbreak ended quickly, with RT-qPCR turning negative. Rapid responses plus biosafety can stop local persistence and viral spread. 3/8
September 29, 2025 at 2:26 PM
In Romania (2024), backyard birds—chickens, geese, ducks, pheasants—did not sustain circulation. An H5N1 outbreak ended quickly, with RT-qPCR turning negative. Rapid responses plus biosafety can stop local persistence and viral spread. 3/8
Congrats Debjyoti @nullsimpleton.bsky.social from @ufz.de: great outcome of #RootWayS project.
Interrelating amplicon sequencing, qPCR & proteomics from field samples of #root channels: what a challenge leading to this wonderful outcome @globalchangebio.bsky.social.
Link: dx.doi.org/10.1111/gcb....
Interrelating amplicon sequencing, qPCR & proteomics from field samples of #root channels: what a challenge leading to this wonderful outcome @globalchangebio.bsky.social.
Link: dx.doi.org/10.1111/gcb....
Cover Crop Root Channels Promote Bacterial Adaptation to Drought in the Maize Rhizosphere
In this study, we examine the structural and functional changes in the bacterial communities in the rhizosphere of a summer cash crop (maize) that reuses root channels from winter cover crops to impr...
dx.doi.org
September 25, 2025 at 10:59 PM
Congrats Debjyoti @nullsimpleton.bsky.social from @ufz.de: great outcome of #RootWayS project.
Interrelating amplicon sequencing, qPCR & proteomics from field samples of #root channels: what a challenge leading to this wonderful outcome @globalchangebio.bsky.social.
Link: dx.doi.org/10.1111/gcb....
Interrelating amplicon sequencing, qPCR & proteomics from field samples of #root channels: what a challenge leading to this wonderful outcome @globalchangebio.bsky.social.
Link: dx.doi.org/10.1111/gcb....
I just ran a whole ass qPCR while forgetting to put the plate in the actual instrument. It was in the centrifuge the whole time. I quit science.
September 17, 2025 at 11:06 PM
I just ran a whole ass qPCR while forgetting to put the plate in the actual instrument. It was in the centrifuge the whole time. I quit science.
Ultra low light bio-optical ULS24 CMOS sensor incorporated into ultra portable qPCR detection system ()
November 18, 2024 at 11:01 PM
Ultra low light bio-optical ULS24 CMOS sensor incorporated into ultra portable qPCR detection system ()
I'm too nervous about our qPCR samples that are running to be able to write.
September 4, 2024 at 8:06 PM
I'm too nervous about our qPCR samples that are running to be able to write.
I ran qPCR today... Happy to report I am still solid bench material! Got cool data! Happy #newPI
Feels good to do what you trained for !
Feels good to do what you trained for !
December 4, 2024 at 11:50 PM
I ran qPCR today... Happy to report I am still solid bench material! Got cool data! Happy #newPI
Feels good to do what you trained for !
Feels good to do what you trained for !
Nobody cares about trad qualifications in the biohacking world. 😋
But e.g. trad qualifications: B.S., ~3 yrs of wet lab and surgical experience in academia including gonadectomies, brain harvesting, stereotactic brain surgery, brain staining, RNA extraction (RT-PCR), qPCR, etc.
But e.g. trad qualifications: B.S., ~3 yrs of wet lab and surgical experience in academia including gonadectomies, brain harvesting, stereotactic brain surgery, brain staining, RNA extraction (RT-PCR), qPCR, etc.
May 3, 2023 at 5:40 PM
Nobody cares about trad qualifications in the biohacking world. 😋
But e.g. trad qualifications: B.S., ~3 yrs of wet lab and surgical experience in academia including gonadectomies, brain harvesting, stereotactic brain surgery, brain staining, RNA extraction (RT-PCR), qPCR, etc.
But e.g. trad qualifications: B.S., ~3 yrs of wet lab and surgical experience in academia including gonadectomies, brain harvesting, stereotactic brain surgery, brain staining, RNA extraction (RT-PCR), qPCR, etc.
had tissue-level analysis done to examine which cells took up the mRNA-LNPs. If you guessed it was predominantly antigen-presenting cells that took them up (as has been shown in every study looking at this question), you would be right. Interestingly, some uptake was also seen in B cells.
January 12, 2025 at 3:17 PM
had tissue-level analysis done to examine which cells took up the mRNA-LNPs. If you guessed it was predominantly antigen-presenting cells that took them up (as has been shown in every study looking at this question), you would be right. Interestingly, some uptake was also seen in B cells.
New FDA statement after WaPo report re: detection H5N1 in milk supply
"qPCR findings do not represent actual virus that may be a risk to consumers"
"assessing any positive findings through egg inoculation tests, a gold-standard for determining viable virus"
tinalexander.github.io/notes/attach...
"qPCR findings do not represent actual virus that may be a risk to consumers"
"assessing any positive findings through egg inoculation tests, a gold-standard for determining viable virus"
tinalexander.github.io/notes/attach...
April 23, 2024 at 10:28 PM
New FDA statement after WaPo report re: detection H5N1 in milk supply
"qPCR findings do not represent actual virus that may be a risk to consumers"
"assessing any positive findings through egg inoculation tests, a gold-standard for determining viable virus"
tinalexander.github.io/notes/attach...
"qPCR findings do not represent actual virus that may be a risk to consumers"
"assessing any positive findings through egg inoculation tests, a gold-standard for determining viable virus"
tinalexander.github.io/notes/attach...
Because we all have dNTPs, primers, probe, Tag enzyme, RT enzyme, RNA isolation kit, table centrifuge and of course a good qPCR machine at home ... 🤔 right? 🤭🤦♂️
June 21, 2025 at 10:13 PM
Because we all have dNTPs, primers, probe, Tag enzyme, RT enzyme, RNA isolation kit, table centrifuge and of course a good qPCR machine at home ... 🤔 right? 🤭🤦♂️
Is it weird for vendors to reach out with special birthday discounts on qPCR reagents because it is your qPCR machine's 10th birthday?
Because I feel like that's weird.
And yet, here we are.
Because I feel like that's weird.
And yet, here we are.
July 16, 2025 at 8:38 PM
Is it weird for vendors to reach out with special birthday discounts on qPCR reagents because it is your qPCR machine's 10th birthday?
Because I feel like that's weird.
And yet, here we are.
Because I feel like that's weird.
And yet, here we are.
Molly Jones from @kent.ac.uk speaking on a novel #eDNA model that accounts for qPCR time series data, and for contamination and inhibition. #UKDNAWG25
May 13, 2025 at 3:12 PM
Molly Jones from @kent.ac.uk speaking on a novel #eDNA model that accounts for qPCR time series data, and for contamination and inhibition. #UKDNAWG25
Little scientist ego boost today when my custom-designed qPCR primer pairs for GAPDH were all within acceptable efficiency range, except for the IDT "ready-made" pair that I purchased on the side, which was by far the least efficient. Also - despair that people are wasting money on a bad pair!
May 15, 2025 at 6:00 PM
Little scientist ego boost today when my custom-designed qPCR primer pairs for GAPDH were all within acceptable efficiency range, except for the IDT "ready-made" pair that I purchased on the side, which was by far the least efficient. Also - despair that people are wasting money on a bad pair!
In both mouse & human data sets, B:H ratios aligned with qPCR data and diet-derived read normalization.
Additionally, we found data from a milk microbiome study, where they used a spike-in to estimate total bacterial biomass. Spike-in and B:H biomass estimates showed almost perfect agreement
Additionally, we found data from a milk microbiome study, where they used a spike-in to estimate total bacterial biomass. Spike-in and B:H biomass estimates showed almost perfect agreement
July 31, 2025 at 3:33 PM
In both mouse & human data sets, B:H ratios aligned with qPCR data and diet-derived read normalization.
Additionally, we found data from a milk microbiome study, where they used a spike-in to estimate total bacterial biomass. Spike-in and B:H biomass estimates showed almost perfect agreement
Additionally, we found data from a milk microbiome study, where they used a spike-in to estimate total bacterial biomass. Spike-in and B:H biomass estimates showed almost perfect agreement
Other vials were opened and used, with some remenants refrozen and shipped. This will affect the quality of the mRNA.
Plasmid DNA was detected via qPCR with Ori, Spike and SV40 promoter probes. Strangely, the 3 probes give different content of DNA, which should not happen.
6/8
Plasmid DNA was detected via qPCR with Ori, Spike and SV40 promoter probes. Strangely, the 3 probes give different content of DNA, which should not happen.
6/8
September 6, 2025 at 5:35 PM
Other vials were opened and used, with some remenants refrozen and shipped. This will affect the quality of the mRNA.
Plasmid DNA was detected via qPCR with Ori, Spike and SV40 promoter probes. Strangely, the 3 probes give different content of DNA, which should not happen.
6/8
Plasmid DNA was detected via qPCR with Ori, Spike and SV40 promoter probes. Strangely, the 3 probes give different content of DNA, which should not happen.
6/8
Mussels on the move: new records of the invasive non-native quagga mussel (Dreissena rostriformis bugensis) in Great Britain using eDNA and a new probe-based qPCR assay https://www.biorxiv.org/content/10.1101/2023.12.18.572119v1
Mussels on the move: new records of the invasive non-native quagga mussel (Dreissena rostriformis bugensis) in Great Britain using eDNA and a new probe-based qPCR assay https://www.biorxiv.org/content/10.1101/2023.12.18.572119v1
Invasive non-native species (INNS) pose a worldwide environmental threat, negatively impacting invad
www.biorxiv.org
December 19, 2023 at 1:40 AM
Mussels on the move: new records of the invasive non-native quagga mussel (Dreissena rostriformis bugensis) in Great Britain using eDNA and a new probe-based qPCR assay https://www.biorxiv.org/content/10.1101/2023.12.18.572119v1
50% Cash Back on #NZYTech #PCR/#qPCR Polymerases & Master Mixes
Buy £300+ enzymes, kits & other #reagents from selected products:
tinyurl.com/3c4vvxk4
receive credit for 50% of the buying value to put towards buying selected NZYTech products from Jan to March '25
Ends 31/12/24
Buy £300+ enzymes, kits & other #reagents from selected products:
tinyurl.com/3c4vvxk4
receive credit for 50% of the buying value to put towards buying selected NZYTech products from Jan to March '25
Ends 31/12/24
December 18, 2024 at 12:45 PM
50% Cash Back on #NZYTech #PCR/#qPCR Polymerases & Master Mixes
Buy £300+ enzymes, kits & other #reagents from selected products:
tinyurl.com/3c4vvxk4
receive credit for 50% of the buying value to put towards buying selected NZYTech products from Jan to March '25
Ends 31/12/24
Buy £300+ enzymes, kits & other #reagents from selected products:
tinyurl.com/3c4vvxk4
receive credit for 50% of the buying value to put towards buying selected NZYTech products from Jan to March '25
Ends 31/12/24