**Abstract:** This paper presents a novel approach to constructing functional, scalable artificial vascular networks utilizing 3D-bioprinted scaffolds and dynamic micro-environment gradient engineering. Building upon established principles of biomaterial science, microfluidics, and cellular differentiation, we introduce a closed-loop feedback system that dynamically adjusts oxygen and growth factor concentrations within a bioprinted scaffold, driving directed angiogenesis and vessel maturation. The system, dubbed the Gradient-Adaptive Vascularization Engine (GAVE), overcomes limitations of static gradient approaches by responding in real-time to cellular behavior and perfusion conditions.

Marquardt, Jonas; Vockert, Niklas; Behrenbruch, Niklas; et al. Alzheimer's and dementia Vol. 21 Suppl 9, no. Suppl 9, p. e110578

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Brown adipose tissue is extensively vascularized, which is essential for its physiological activities. Li et al. show that nutrient/stress-sensing O-GlcNAc signaling in brown adipocytes dynamically adapts to environmental stimuli to stimulate brown adipose tissue vascularization and thermogenesis via a KCP-mediated paracrine mechanism.

**Abstract:** Current 3D bioprinting methods for bone regeneration often struggle with achieving robust vascularization, hindering nutrient diffusion and osteointegration. This work proposes a novel methodology leveraging stochastically generated, hierarchical vascular patterns within the bioink matrix, guided by a self-optimizing, physics-informed reinforcement learning (RL) framework. Our approach dramatically improves vascular network density and organization, resulting in accelerated osteointegration and enhanced scaffold mechanical properties for scalable bone tissue engineering applications.

**Abstract:** Current methods for assessing vascularization following spinal cord injury (SCI) in murine models rely on subjective histological analysis, limiting throughput and reproducibility. This paper proposes a novel, fully automated system leveraging multi-modal machine learning (MMML) to quantify scaffold vascularization with high accuracy and speed. The system integrates optical coherence tomography (OCT), immunohistochemistry (IHC), and micro-computed tomography (micro-CT) data to generate a comprehensive vascularization score, facilitating more rigorous and efficient preclinical evaluation of regenerative therapies.

3D bioprinting has revolutionized tissue engineering, enabling intricate, physiologically relevant constructs unattainable with conventional techniques, yet it remains limited in integrating soft and ...