There was a vaccine for Lyme disease, but production was canceled due to insufficient demand. www.cdc.gov/lyme/about/l...

I've heard nice things about jlc3dp.com

Thanks! Yep, we do 3D excitation (z scan with a top hat) and 3D modeling of fluorophore positions. See, e.g., example2.

These and many other simulations are covered in the paper. Example notebooks for each simulation are provided as part of the code base, and on Google Colab (see github.com/ries-lab/Sim...). We hope the community will find this useful and contribute their own simulated examples using this package.

For single molecule tracking, we optimized laser power, pattern size, photon limit and pattern dwell time to increase the measurable diffusion coefficient by 70% over that captured by the standard Abberior tracking sequence.