We’re bringing our latest progress in neuromodulator imaging to #SfN2025! Don’t miss Dr. Li’s talk (Nov 19) and our lab’s posters (Nov 16). We look forward to connecting, exchanging ideas, and exploring collaborations. #neuroscience #neuroimaging #biosensors @sfn.org

6/ Sustained serotonin release in vCA1 keeps 5-HT2C–expressing pyramidal neuron ensembles, linking temporally separated events. When the gap exceeds the brain’s “safe window”, brief serotonin release fails to drive these ensembles, thus preventing maladaptive learning.

3/ We found that serotonin is the key.
In trace fear conditioning, systemic serotonin manipulations bidirectionally shift the associable interval. Using our 5-HT3.0 sensor, we showed real-time, in vivo evidence that serotonin release patterns in vCA1 tightly track this time window.

(3/3) Applying Cort1.0 in vivo: We observed stress-induced 😰CORT elevation in the hypothalamus (e.g., during tail suspension) with Cort1.0 by fiber photometry. Real-time stress hormone readouts, live!

(2/3) Applying Prog1.0 in vivo: We detected both the maternal behavior-associated🤱 and spontaneous PROG signals ☀️🌙in the hypothalamus with Prog1.0 by fiber photometry.

(1/3) Excited to introduce our new GRAB sensors for a series of steroid hormones! These tools enable real-time detection of steroid hormone dynamics in vivo🐭🧠. Happy to share these sensors and welcome any feedback! Please contact [email protected] for information.

Join the "Genetically-Encoded and Chemigenetic Tools for Analysis and Control of Biological Systems" symposium at #ChemPacific2025! Explore cutting-edge tools alongside other world-class science in Hawaii. 🌴🌴
pacifichem.org

Thrilled to share our latest research published in Nature Communications! Our new GRAB sensor of SNPF promotes discoveries in the dynamics and molecular regulation between neuropeptide and neurotransmitter release in vivo. Check out the full paper here: www.nature.com/articles/s41...

(4/6) Through multiplex imaging using 2P microscopy, we've explored the dynamics of ACh and norepinephrine in the visual cortex across various behaviors. rACh1h responds to both water licking and forced running, while NE2m responds only to forced running.

(3/6) With fiber photometry, we've shown the ability to perform multiplex recordings of ACh and dopamine signals during Pavlovian conditioning tasks, and distinct dynamics between ACh and serotonin during REM sleep.

(2/6) These sensors are rationally engineered with a chimeric GPCR strategy, providing a superior signal-to-noise ratio compared to existing green ACh sensors.

(5/7) The major breakthrough of this study is the simultaneous multi-color recording of spontaneous and behavior-associated DA, ACh, and cAMP signals in the NAc region in living mice, providing unprecedented insights into complex neurochemical networks.

(4/7) In zebrafish, we achieved 3-color in vivo imaging by expressing HaloDA1.0 with the red Ca2+ sensor and the green ATP sensor. We observed coordinated activation patterns during electrical shocks and acute seizure attack

(3/7) In acute brain slices, we achieved simultaneous imaging of three key neuromodulators—DA, ACh, and eCB release—following electrical stimulation and pharmacological interventions

(2/7) This sensor combines our GPCR activation-based (GRAB) strategy with the cpHaloTag-dye approach, providing high sensitivity and a spectral range from far-red to NIR.