Even with the largest zoom on a classic compound optical system, scientists struggle to make sense of finer details. Microbiologists are turning to an unexpected source to clear things up: a moisture-absorbing material found in diapers. www.quantamagazine.org/expansion-mi...

Isadonna Tengganu and Ke Hu develop transfection and ExM protocols for Chromera velia, enabling direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondi.
journals.biologists.com/jcs/article/...

But none of our existing imaging methods (SoRa, STORM…) could really visualize integration within the bundled microtubules in neurites. So Ciarán set up U-ExM expansion microscopy, then performed it on microinjected neurons - now we could see plus-end growth and in-lattice renewal everywhere! 🧵 7/9

Introducing Cyclically Multiplexed Expansion Microscopy (Cy-ExM): a workflow for 3D nanoscale, high-plex imaging in whole cells. Cryo-preserved ultrastructure + iterative labeling + expansion microscopy → 20 targets in one dataset with ~70 nm lateral resolution.

www.biorxiv.org/content/10.6...

Last chance to apply for 'Ultrastructure expansion microscopy: from cells to tissue' yet? #EMBOExM

🔬 Advance your microscopy skills by joining us to explore U-ExM techniques and gain hands-on expertise in applying this powerful approach to mammalian cells.

Apply now: s.embl.org/uex26-01-bl

Are you staining DNA in your ExM samples?

We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.

You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.

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