Background Genome spatial organization plays a fundamental role in biological function across all domains of life. While the principles of nuclear architecture have been well-characterized in animals ...

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Université de Bordeaux

After sensing capsids, the Thoeris antiphage defense system triggers a cascade that leads to NAD+ cleavage and cell arrest, which are dynamics that are mirrored in some mammalian immune pathways.

Investigating small-molecule dynamics within microbes is essential for comprehensive studies of microbial function. Both intra-organism and inter-organism small molecule dynamics play critical roles in microbial physiology, symbiosis, and disease. However, monitoring these dynamics remains highly challenging using most existing techniques. Fluorophore-based genetically encoded biosensors are powerful tools for tracking small-molecule dynamics in vivo and hold high potential for driving new discoveries. These biosensors are most commonly used in fluorescence imaging, often in combination with perfusion devices that allow precise control over environmental conditions. When integrated with advanced imaging techniques, this approach provides high-resolution, spatially and temporally resolved data, enabling insights into single-cell microbial responses. Despite their promise, implementing such biosensors remains technically challenging. Understanding the key steps is crucial for broader adoption. Here, we present a protocol designed to support the effective deployment of newly engineered biosensors into microbes for quantitative ratiometric fluorescence imaging under controlled conditions.

Dans un article publié dans Current Biology, des scientifiques explorent comment l’auxine, une hormone végétale, voyage dans la tige d’une mousse pou

Chez les organismes multicellulaires, l'équilibre entre l’activation et la répression des gènes est crucial pour le développement.

The rapid detection of a pathogen in a new environment and/or in a new host plant is essential to mitigate disease outbreaks. However, bacteria with a wide plant host range, many asymptomatic hosts, a...