Tomáš Heger
@tomasheger.bsky.social
Postdoc in Elena Seiradake's lab, Oxford Biochem (PhD @ Poul Nissen's lab, Århus, 🇩🇰)
Reposted by Tomáš Heger
Structural basis of modified ligand selectivity from N-terminal PAC1R alternative splicing pubmed.ncbi.nlm.nih.gov/41264251/ #cryoem
November 21, 2025 at 7:40 PM
Structural basis of modified ligand selectivity from N-terminal PAC1R alternative splicing pubmed.ncbi.nlm.nih.gov/41264251/ #cryoem
Reposted by Tomáš Heger
Yay! Our findings on the antidepressant drug vilazodone binding to the serotonin transporter (SERT) are out! We used cryo-electron microscopy and other advanced biophysics to provide data suggesting two high affinity binding poses.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structural basis of vilazodone dual binding mode to the serotonin transporter - Nature Communications
The serotonin transporter (SERT) is a key antidepressant target. Here, authors present a cryo-EM structure where the antidepressant vilazodone spans the main SERT binding site toward a secondary ...
www.nature.com
November 23, 2025 at 7:48 PM
Yay! Our findings on the antidepressant drug vilazodone binding to the serotonin transporter (SERT) are out! We used cryo-electron microscopy and other advanced biophysics to provide data suggesting two high affinity binding poses.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Tomáš Heger
At some point, Doppio will replace relion's gui. Already you can do more in Doppio than in relion! 🤩
We are pleased to announce Doppio version 1.4! Available here www.ccpem.ac.uk/software/dow...
Downloads – CCP-EM
www.ccpem.ac.uk
November 4, 2025 at 6:14 PM
At some point, Doppio will replace relion's gui. Already you can do more in Doppio than in relion! 🤩
Reposted by Tomáš Heger
Frequent user of epitope tags? Try ours!
IPI epitope tag antibodies & plasmids are now available through our partner @addgene.bsky.social!
buff.ly/YEuZDIp
IPI epitope tag antibodies & plasmids are now available through our partner @addgene.bsky.social!
buff.ly/YEuZDIp
Epitope tag antibody collection
Epitope tags are short amino acid sequences fused to a protein’s N- or C-terminus. When paired with an antibody that specifically recognizes this sequence, epit
buff.ly
September 23, 2025 at 6:02 PM
Frequent user of epitope tags? Try ours!
IPI epitope tag antibodies & plasmids are now available through our partner @addgene.bsky.social!
buff.ly/YEuZDIp
IPI epitope tag antibodies & plasmids are now available through our partner @addgene.bsky.social!
buff.ly/YEuZDIp
Reposted by Tomáš Heger
Our paper on SLC45A4 is out now in @nature.com! The orphan SLC45A4 transporter is a polyamine transporter involved in chronic pain signalling!
SLC45A4 is a pain gene encoding a neuronal polyamine transporter - Nature
The SLC45A4 gene encodes a neuronal polyamine transporter and is linked to pain response in humans and mice.
www.nature.com
August 21, 2025 at 11:05 AM
Our paper on SLC45A4 is out now in @nature.com! The orphan SLC45A4 transporter is a polyamine transporter involved in chronic pain signalling!
Reposted by Tomáš Heger
I am excited to share our latest preprint presenting the structure of human NHE6, a key endosomal Na⁺/H⁺ exchanger involved in Christianson syndrome.
We bring together #cryo-EM, NMR, SAXS, lipidomics, and functional assays for an integrative analysis of NHE6 architecture, function, and regulation.
We bring together #cryo-EM, NMR, SAXS, lipidomics, and functional assays for an integrative analysis of NHE6 architecture, function, and regulation.
July 28, 2025 at 9:31 PM
I am excited to share our latest preprint presenting the structure of human NHE6, a key endosomal Na⁺/H⁺ exchanger involved in Christianson syndrome.
We bring together #cryo-EM, NMR, SAXS, lipidomics, and functional assays for an integrative analysis of NHE6 architecture, function, and regulation.
We bring together #cryo-EM, NMR, SAXS, lipidomics, and functional assays for an integrative analysis of NHE6 architecture, function, and regulation.
Check out this fascinating study on a fungal multipass translocon! What's really neat is how they did it: cryo-EM structure from the endogenously tagged and purified SND3 complex. It will be exciting to see if there is any potential for SBDD against fungi, Trypanosoma, and Leishmania (?).
Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...
July 12, 2025 at 3:51 PM
Check out this fascinating study on a fungal multipass translocon! What's really neat is how they did it: cryo-EM structure from the endogenously tagged and purified SND3 complex. It will be exciting to see if there is any potential for SBDD against fungi, Trypanosoma, and Leishmania (?).
Reposted by Tomáš Heger
We are excited to present the first #cryoEM structures of human GABA transporter 3 – GAT3 – bound to an in-house selective inhibitor, SR-THAP, to substrate GABA, and in the substrate-free state!
@biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
@biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
Structural basis for selective inhibition of human GABA transporter GAT3
The astrocytic γ-aminobutyric acid (GABA) transporter, GAT3, is essential for terminating GABAergic signalling in the central nervous system. Selective inhibition of GAT3 offers a potential strategy f...
www.biorxiv.org
March 29, 2025 at 2:12 PM
We are excited to present the first #cryoEM structures of human GABA transporter 3 – GAT3 – bound to an in-house selective inhibitor, SR-THAP, to substrate GABA, and in the substrate-free state!
@biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
@biorxivpreprint.bsky.social
www.biorxiv.org/content/10.1...
Yes, please. I go with Nemesis drones and like their motto "to see means to win":
www.dronynemesis.cz/en.
www.dronynemesis.cz/en.
March 9, 2025 at 1:56 AM
Yes, please. I go with Nemesis drones and like their motto "to see means to win":
www.dronynemesis.cz/en.
www.dronynemesis.cz/en.
Oh yeah, P-types ❤️💙💛 and the copper one, ATP7B!
It was a great day full of science. Thank you so much everyone for being involved!
It was a great day full of science. Thank you so much everyone for being involved!
Huge congrats to @tomasheger.bsky.social who succesfully defended his PhD yesterday.
Such a joy to supervise with @tdieudonne.bsky.social and @poulnissen.bsky.social.
Big thank you to @melaniemcdowell.bsky.social, @henrietteautzen.bsky.social and Daan van Aalten for a great discussion.
P-type ❤️💙💛
Such a joy to supervise with @tdieudonne.bsky.social and @poulnissen.bsky.social.
Big thank you to @melaniemcdowell.bsky.social, @henrietteautzen.bsky.social and Daan van Aalten for a great discussion.
P-type ❤️💙💛
January 18, 2025 at 11:55 PM
Oh yeah, P-types ❤️💙💛 and the copper one, ATP7B!
It was a great day full of science. Thank you so much everyone for being involved!
It was a great day full of science. Thank you so much everyone for being involved!
Thank you so much for guiding me on this amazing journey, @poulnissen.bsky.social, @charlottstock.bsky.social, and @tdieudonne.bsky.social. So great and thorough discussion with @melaniemcdowell.bsky.social, @henrietteautzen.bsky.social and Daan van Aalten. I highly appreciate your involvement.
🎉Congrats @tomasheger.bsky.social for this excellent PhD defense. You were the expert in the room. Very very proud supervisors with @charlottstock.bsky.social and @poulnissen.bsky.social ! Many thanks to the jury members Daan van Aalten, @henrietteautzen.bsky.social and @melaniemcdowell.bsky.social
January 18, 2025 at 11:42 PM
Thank you so much for guiding me on this amazing journey, @poulnissen.bsky.social, @charlottstock.bsky.social, and @tdieudonne.bsky.social. So great and thorough discussion with @melaniemcdowell.bsky.social, @henrietteautzen.bsky.social and Daan van Aalten. I highly appreciate your involvement.