Thomas Balan
@thomasbalan.bsky.social
Postdoc in the Duharcourt lab at the Jacques Monod Institute
Sudying histone modifications during programmed DNA elimination in Paramecium
PhD from @upcite.bsky.social @ijmonod.bsky.social
Sudying histone modifications during programmed DNA elimination in Paramecium
PhD from @upcite.bsky.social @ijmonod.bsky.social
Congrats Andreas!!
October 30, 2025 at 9:35 AM
Congrats Andreas!!
@ijmonod.bsky.social @i2bcparissaclay.bsky.social @frm-officiel.bsky.social @EUR_G.E.N.E @upcite.bsky.social
October 21, 2025 at 2:46 PM
This is part of my PhD work that I recently concluded in the lab of @sandraduharcourt.bsky.social 🎓 🎓
Huge thanks to everyone who contributed to this story (Mélanie, Marc & Valério, who are co-authors with me on this project !) as well as all collaborators for their dedication and insights. 🎉
Huge thanks to everyone who contributed to this story (Mélanie, Marc & Valério, who are co-authors with me on this project !) as well as all collaborators for their dedication and insights. 🎉
October 21, 2025 at 2:46 PM
This is part of my PhD work that I recently concluded in the lab of @sandraduharcourt.bsky.social 🎓 🎓
Huge thanks to everyone who contributed to this story (Mélanie, Marc & Valério, who are co-authors with me on this project !) as well as all collaborators for their dedication and insights. 🎉
Huge thanks to everyone who contributed to this story (Mélanie, Marc & Valério, who are co-authors with me on this project !) as well as all collaborators for their dedication and insights. 🎉
By revealing how condensin I assists a domesticated transposase in sculpting the somatic genome, this work opens the door to exploring how genome organisation shapes DNA elimination in Paramecium 🤩
October 21, 2025 at 2:46 PM
By revealing how condensin I assists a domesticated transposase in sculpting the somatic genome, this work opens the door to exploring how genome organisation shapes DNA elimination in Paramecium 🤩
👉 To conclude, we show that condensin I physically and functionally interacts with PiggyMac, thus highlighting a strong interaction between the two machineries.
October 21, 2025 at 2:46 PM
👉 To conclude, we show that condensin I physically and functionally interacts with PiggyMac, thus highlighting a strong interaction between the two machineries.
🧬 We demonstrate that this new development-specific complex is essential for DNA elimination and the establishment of H3K9me3 & H3K27me3 patterns during development, fully phenocopying PiggyMac depletion.
October 21, 2025 at 2:46 PM
🧬 We demonstrate that this new development-specific complex is essential for DNA elimination and the establishment of H3K9me3 & H3K27me3 patterns during development, fully phenocopying PiggyMac depletion.
🧪Using TurboID proximity labelling, we identified condensin I subunits among the closest interactors of the PiggyMac endonuclease, leading to the identification of a development-specific condensin I complex
October 21, 2025 at 2:46 PM
🧪Using TurboID proximity labelling, we identified condensin I subunits among the closest interactors of the PiggyMac endonuclease, leading to the identification of a development-specific condensin I complex
During Paramecium development, 30% of the germline genome, including TEs, is precisely eliminated to form a functional somatic genome.
The endonuclease PiggyMac and its partners catalyse this process, but how they are targeted to DNA is unknown. (see doi.org/10.1002/wrna... for more)
The endonuclease PiggyMac and its partners catalyse this process, but how they are targeted to DNA is unknown. (see doi.org/10.1002/wrna... for more)
Small‐RNA‐guided histone modifications and somatic genome elimination in ciliates
In ciliates, an ancestral pathway for transposable element repression by small RNAs and histone methyltransferases has evolved into a highly orchestrated process to eliminate unwanted sequences from ...
doi.org
October 21, 2025 at 2:46 PM
During Paramecium development, 30% of the germline genome, including TEs, is precisely eliminated to form a functional somatic genome.
The endonuclease PiggyMac and its partners catalyse this process, but how they are targeted to DNA is unknown. (see doi.org/10.1002/wrna... for more)
The endonuclease PiggyMac and its partners catalyse this process, but how they are targeted to DNA is unknown. (see doi.org/10.1002/wrna... for more)