New! 15y after the classic Aigouy et al "Cell Flow Reorients the Axis of Planar Polarity in the Wing Epithelium..." from lab of much missed Suzanne Eaton, we provide our take from post-doc Sara Tan "Tissue shear as a cue for aligning planar polarity in the developing Drosophila wing".
rdcu.be/d81Vn

Drosophila cell culture people: we've been using Qiagen Effectene to transfect S2 cells with ~30% efficiency, but latest batch is only giving us <1% efficiency (so no use to us!!). Qiagen don't seem to have a solution.

Anyone got suggestions for other (good!) fly cell culture transfection reagents?

Dodged that pre- Christmas bullet. Mycoplasma, not Covid.

Our latest on planar polarity mechanisms. Painstaking work by Alexandre Carayon, investigating mechanisms of cell-scale polarisation in the fly wing. Enjoy it (if this is your thing)!

For my planar polarity follower(s?), we have a new paper out on Fat-Dachsous.

Fat-Dachsous are known to interact (signal?) in trans between cells, but we now find a second (more important!) trans-interaction suggesting new models for Fat-Dachsous clustering

www.sciencedirect.com/science/arti...

Bluesky exclusive (at least until I get around to posting on the other place) – our latest work on understanding symmetry breaking in planar polarity:
www.sciencedirect.com/science/arti...
I would do a lengthy 'tweetorial' but luckily for you all I don't know how to.

Here for the science. But in the meantime for my friends who think it always rains in Sheffield, some pictures from Stanage Edge this morning.