Christian Steinmetzger
@stonebutcher.bsky.social
Senior research engineer in the group of Katja Petzold at Uppsala University: https://petzoldlab.com/
Interested in RNA chemical biology and structure, even better if some NMR is involved
Interested in RNA chemical biology and structure, even better if some NMR is involved
With a 250 instead of 200 ms the experiment went to completion. Still curious that it used to work with 200 ms.
I also tested the 15N sofast-HMQC library pp sfhmqcf3gpph, with 128 increments, 125 ms D1, 71 ms AQ, and that completed normally. So only the 13C pp sf_mehmqcgpph seems to be affected
I also tested the 15N sofast-HMQC library pp sfhmqcf3gpph, with 128 increments, 125 ms D1, 71 ms AQ, and that completed normally. So only the 13C pp sf_mehmqcgpph seems to be affected
November 20, 2025 at 9:08 AM
With a 250 instead of 200 ms the experiment went to completion. Still curious that it used to work with 200 ms.
I also tested the 15N sofast-HMQC library pp sfhmqcf3gpph, with 128 increments, 125 ms D1, 71 ms AQ, and that completed normally. So only the 13C pp sf_mehmqcgpph seems to be affected
I also tested the 15N sofast-HMQC library pp sfhmqcf3gpph, with 128 increments, 125 ms D1, 71 ms AQ, and that completed normally. So only the 13C pp sf_mehmqcgpph seems to be affected
Pulse lengths (~8 us 1H 90°) and AQ (50 ms, with 720 points over 12 ppm) and 30 ms disk i/o delay are the same as always.
The experiment was already done with digital acquisition mode. Using baseopt also lead to an early abort + the IPSO error below that forced a restart.
The experiment was already done with digital acquisition mode. Using baseopt also lead to an early abort + the IPSO error below that forced a restart.
November 20, 2025 at 9:05 AM
Pulse lengths (~8 us 1H 90°) and AQ (50 ms, with 720 points over 12 ppm) and 30 ms disk i/o delay are the same as always.
The experiment was already done with digital acquisition mode. Using baseopt also lead to an early abort + the IPSO error below that forced a restart.
The experiment was already done with digital acquisition mode. Using baseopt also lead to an early abort + the IPSO error below that forced a restart.
For some more details:
- TS 3.8.0
- D1 of 200 ms, AQ 50 ms in the direct dimension
- indirect TD of 160, typically aborts after 50-70
- With a lower NS, e.g. 8 instead of 720, the experiment goes to completion
- A combination of low NS and shorter D1 (e.g. 8 scans with 100 ms) also errors out again
- TS 3.8.0
- D1 of 200 ms, AQ 50 ms in the direct dimension
- indirect TD of 160, typically aborts after 50-70
- With a lower NS, e.g. 8 instead of 720, the experiment goes to completion
- A combination of low NS and shorter D1 (e.g. 8 scans with 100 ms) also errors out again
November 19, 2025 at 6:50 PM
For some more details:
- TS 3.8.0
- D1 of 200 ms, AQ 50 ms in the direct dimension
- indirect TD of 160, typically aborts after 50-70
- With a lower NS, e.g. 8 instead of 720, the experiment goes to completion
- A combination of low NS and shorter D1 (e.g. 8 scans with 100 ms) also errors out again
- TS 3.8.0
- D1 of 200 ms, AQ 50 ms in the direct dimension
- indirect TD of 160, typically aborts after 50-70
- With a lower NS, e.g. 8 instead of 720, the experiment goes to completion
- A combination of low NS and shorter D1 (e.g. 8 scans with 100 ms) also errors out again
If you are there: come see me at poster 110 on Tuesday and Thursday to learn about new developments in nucleic acid DNP measurements
April 6, 2025 at 6:35 PM
If you are there: come see me at poster 110 on Tuesday and Thursday to learn about new developments in nucleic acid DNP measurements
The data browser used to be quite unreliable and slow for me in 4.x (at least on MacOS). TS 4.4.1 has significantly improved on that and now it works well again
November 23, 2024 at 7:30 AM
The data browser used to be quite unreliable and slow for me in 4.x (at least on MacOS). TS 4.4.1 has significantly improved on that and now it works well again