Sara Rouhanifard
@srouhanifard.bsky.social
Asst. Prof of bioengineering @Northeastern University | RNA modifications | RNA localization | click chemistry | single-molecule studies | www.rouhanifardlab.com
3/ But that other 13%?
☑️ Jurkat-specific ψ-sites hit oncogenic and immune activation genes
☑️ Primary T cell–specific ψ-sites appear on trafficking and calcium signaling genes
🧬 Immortalized cells also showed more clustered ψ patterns, hinting at altered control compared to primary T cells.
☑️ Jurkat-specific ψ-sites hit oncogenic and immune activation genes
☑️ Primary T cell–specific ψ-sites appear on trafficking and calcium signaling genes
🧬 Immortalized cells also showed more clustered ψ patterns, hinting at altered control compared to primary T cells.
July 17, 2025 at 5:22 PM
3/ But that other 13%?
☑️ Jurkat-specific ψ-sites hit oncogenic and immune activation genes
☑️ Primary T cell–specific ψ-sites appear on trafficking and calcium signaling genes
🧬 Immortalized cells also showed more clustered ψ patterns, hinting at altered control compared to primary T cells.
☑️ Jurkat-specific ψ-sites hit oncogenic and immune activation genes
☑️ Primary T cell–specific ψ-sites appear on trafficking and calcium signaling genes
🧬 Immortalized cells also showed more clustered ψ patterns, hinting at altered control compared to primary T cells.
2/ We were genuinely surprised by this result — we expected transformation to cause widespread disruption. But 87% of ψ-sites were shared between Jurkat and primary T cells!
Most differences came from gene expression, not ψ-site selection.
This process seems to be tightly conserved.
Most differences came from gene expression, not ψ-site selection.
This process seems to be tightly conserved.
July 17, 2025 at 5:22 PM
2/ We were genuinely surprised by this result — we expected transformation to cause widespread disruption. But 87% of ψ-sites were shared between Jurkat and primary T cells!
Most differences came from gene expression, not ψ-site selection.
This process seems to be tightly conserved.
Most differences came from gene expression, not ψ-site selection.
This process seems to be tightly conserved.
1/🧵
🚨 New paper published in RNA!
Scientists often say anecdotally that RNA modifications are disrupted in immortalized cells — but no one’s really tested it.
So we did: ψ-mapping in primary T cells vs. Jurkat cells using direct RNA-seq.
📄 tinyurl.com/TCellPsiRNA
#nanopore #RNA #pseudouridine
🚨 New paper published in RNA!
Scientists often say anecdotally that RNA modifications are disrupted in immortalized cells — but no one’s really tested it.
So we did: ψ-mapping in primary T cells vs. Jurkat cells using direct RNA-seq.
📄 tinyurl.com/TCellPsiRNA
#nanopore #RNA #pseudouridine
July 17, 2025 at 5:22 PM
1/🧵
🚨 New paper published in RNA!
Scientists often say anecdotally that RNA modifications are disrupted in immortalized cells — but no one’s really tested it.
So we did: ψ-mapping in primary T cells vs. Jurkat cells using direct RNA-seq.
📄 tinyurl.com/TCellPsiRNA
#nanopore #RNA #pseudouridine
🚨 New paper published in RNA!
Scientists often say anecdotally that RNA modifications are disrupted in immortalized cells — but no one’s really tested it.
So we did: ψ-mapping in primary T cells vs. Jurkat cells using direct RNA-seq.
📄 tinyurl.com/TCellPsiRNA
#nanopore #RNA #pseudouridine
4/6 📊 Plasticity vs stability: ~30% of Ψ sites changed occupancy across conditions (“plastic”), while the rest remained constant (“static”). Among the plastic sites was a key Ψ site in YTHDF1—an m6A reader involved in translation, which changed in response to cellular and environmental cues.
March 21, 2025 at 3:42 PM
4/6 📊 Plasticity vs stability: ~30% of Ψ sites changed occupancy across conditions (“plastic”), while the rest remained constant (“static”). Among the plastic sites was a key Ψ site in YTHDF1—an m6A reader involved in translation, which changed in response to cellular and environmental cues.
1/6 🧬New paper at @cp-cellsystems.bsky.social led by @sashafanari.bsky.social! Does pseudouridine (Ψ) dynamically respond to cell state? We used nanopore DRS + Mod-p ID to map Ψ in neuron-like cells under “normal” and “perturbed” conditions (healthy differentiation and unhealthy Pb2+ poisoning).
March 21, 2025 at 3:42 PM
1/6 🧬New paper at @cp-cellsystems.bsky.social led by @sashafanari.bsky.social! Does pseudouridine (Ψ) dynamically respond to cell state? We used nanopore DRS + Mod-p ID to map Ψ in neuron-like cells under “normal” and “perturbed” conditions (healthy differentiation and unhealthy Pb2+ poisoning).
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February 21, 2025 at 2:39 PM
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