I agree, it would be super interesting to zoom in further into specific regions. We were aiming for detection of low abundance proteins and decided against sectioning further. But definitely the next step, also with mass spec getting more and more sensitive.

This was an excellent collaboration with Michiel Vermeulen. Thanks to @ wilkemeijer.bsky.social, @virginiar-andra.bsky.social & Suzan Stelloo for the amazing work. And of course, to all other authors!

@hubrechtinstitute.bsky.social
@rimls.bsky.social

Key findings:
• Mapped novel oscillatory proteins and transcripts that could play a role in somitogenesis
• Discovered a dynamic, antagonistic ligand–receptor pattern in R-Spondin/LGR signalling
• Perturbing ligand dynamics impairs signalling strength and somite differentiation

Congratulations Simona!!

And what a talk that was!

Thanks :-)

And a great collaboration with @irene-yuting-li.bsky.social and Ben Simons to understand how this coupling affects the somitogenesis.

Yes :-). What really made this project possible in the end was establishing single-cell tracking, which allowed us to quantify both cell cycle and signalling dynamics in single cells of the tissue. I’m very proud of Marek from the lab who set this up (and who started to look for postdoc positions!).