Ian Shih
@shihyyi.bsky.social
Brain imager, Professor and Vice Chair for Research at UNC Neurology; Associate Director UNC Biomedical Research Imaging Center; Director, UNC Center for Animal MRI (http://camri.org/)
We hope this protocol will enable broader adoption of multimodal electrical-based recording + fMRI, bridging information across modalities and species. Please reach out to Tatiana ([email protected]) if you are interested in practicing this protocol.
November 20, 2025 at 2:19 PM
We hope this protocol will enable broader adoption of multimodal electrical-based recording + fMRI, bridging information across modalities and species. Please reach out to Tatiana ([email protected]) if you are interested in practicing this protocol.
6/6 The HDCV software developed in-house at UNC sets analyte-specific waveforms and drives TTL synchronization with fMRI. We include practical parameters for dopamine and oxygen detection, stimulation programming, and transparent analysis steps.
November 20, 2025 at 2:19 PM
6/6 The HDCV software developed in-house at UNC sets analyte-specific waveforms and drives TTL synchronization with fMRI. We include practical parameters for dopamine and oxygen detection, stimulation programming, and transparent analysis steps.
5/6 We also describe noise-cleaning steps: a 4th-order Bessel low-pass filter (2 kHz) to remove MRI-induced high-frequency noise, and TTL-based interleaving within a 100 ms window to prevent RF and gradient interference during FSCV.
November 20, 2025 at 2:18 PM
5/6 We also describe noise-cleaning steps: a 4th-order Bessel low-pass filter (2 kHz) to remove MRI-induced high-frequency noise, and TTL-based interleaving within a 100 ms window to prevent RF and gradient interference during FSCV.
4/6 The protocol details the fabrication of carbon-fiber silica electrodes, MR-compatible hardware setup, stereotaxic implantation and animal care, synchronized acquisition, and end-to-end preprocessing and analysis - a reproducible 7-day workflow from build to data.
November 20, 2025 at 2:17 PM
4/6 The protocol details the fabrication of carbon-fiber silica electrodes, MR-compatible hardware setup, stereotaxic implantation and animal care, synchronized acquisition, and end-to-end preprocessing and analysis - a reproducible 7-day workflow from build to data.
3/6 To benchmark the platform before in vivo experiments, we describe the use of a dummy cell to profile noise inside the MRI, and a gravity-driven microfluidic dopamine flow cell with secure waste collection for MR-compatible testing.
November 20, 2025 at 2:16 PM
3/6 To benchmark the platform before in vivo experiments, we describe the use of a dummy cell to profile noise inside the MRI, and a gravity-driven microfluidic dopamine flow cell with secure waste collection for MR-compatible testing.
2/6 What is FSCV? A chemically selective, label-free electrochemical technique that quantifies rapid neurochemical dynamics and has already been applied in humans. Combined with fMRI, it bridges spatial and temporal scales to link molecular events to brain-wide activity.
November 20, 2025 at 2:16 PM
2/6 What is FSCV? A chemically selective, label-free electrochemical technique that quantifies rapid neurochemical dynamics and has already been applied in humans. Combined with fMRI, it bridges spatial and temporal scales to link molecular events to brain-wide activity.
Amazing presentation Alessandro! The series of studies connected so well and I truly enjoyed how the story flowed!
June 28, 2025 at 8:00 AM
Amazing presentation Alessandro! The series of studies connected so well and I truly enjoyed how the story flowed!
Thanks for organizing this Francesca! This will definitely be one of the most memorable parts of the trip!
May 15, 2025 at 6:14 AM
Thanks for organizing this Francesca! This will definitely be one of the most memorable parts of the trip!
On Thursday (May 15) afternoon at 15:33 in the preclinical brain imaging oral session, Liming Hsu will present a chemogenetic fMRI study building on our prior work (www.science.org/doi/10.1126/...), studying how PVT modulates locus coeruleus-induced MD-PrL connectivity and behavior.
May 9, 2025 at 10:31 AM
On Thursday (May 15) afternoon at 15:33 in the preclinical brain imaging oral session, Liming Hsu will present a chemogenetic fMRI study building on our prior work (www.science.org/doi/10.1126/...), studying how PVT modulates locus coeruleus-induced MD-PrL connectivity and behavior.
On Wednesday (May 14) afternoon at 15:45 in the multimodal fMRI oral session, Liming Hsu will cover a recently published story about the specificity of “optogenetic terminal stimulation”: pubmed.ncbi.nlm.nih.gov/40090667/
May 9, 2025 at 10:31 AM
On Wednesday (May 14) afternoon at 15:45 in the multimodal fMRI oral session, Liming Hsu will cover a recently published story about the specificity of “optogenetic terminal stimulation”: pubmed.ncbi.nlm.nih.gov/40090667/
On Wednesday (May 14) afternoon at 13:30 in the preclinical fMRI e-poster session, Weiting Zhang will discuss our initial attempts to resolve what causes synchronous resting-state connectivity in the striatum between two hemispheres.
May 9, 2025 at 10:31 AM
On Wednesday (May 14) afternoon at 13:30 in the preclinical fMRI e-poster session, Weiting Zhang will discuss our initial attempts to resolve what causes synchronous resting-state connectivity in the striatum between two hemispheres.
On Monday (May 12) afternoon at 17:12 in the fMRI acquisition/contrast oral session, Sheng Song will give an overview of SORDINO and its use in awake behaving mice.
May 9, 2025 at 10:31 AM
On Monday (May 12) afternoon at 17:12 in the fMRI acquisition/contrast oral session, Sheng Song will give an overview of SORDINO and its use in awake behaving mice.
On Monday (May 12) afternoon at 16:48 in the Neurofluid oral session, Sungho Lee will present the use of SORDINO on mapping glymphatic dynamics and choroid plexus efflux.
May 9, 2025 at 10:31 AM
On Monday (May 12) afternoon at 16:48 in the Neurofluid oral session, Sungho Lee will present the use of SORDINO on mapping glymphatic dynamics and choroid plexus efflux.
Also on Monday (May 12) morning at 8:15 in the multimodal e-poster session, Liming Hsu will showcase the use of miniscope calcium imaging during SORDINO fMRI and how it may help examine the clusters of cells in PrL linking to distinct large-scale networks.
May 9, 2025 at 10:31 AM
Also on Monday (May 12) morning at 8:15 in the multimodal e-poster session, Liming Hsu will showcase the use of miniscope calcium imaging during SORDINO fMRI and how it may help examine the clusters of cells in PrL linking to distinct large-scale networks.
On Monday (May 12) morning at 8:15 in the multimodal e-poster session, Tatiana Shnitko will discuss the use of fMRI, electrophysiology, and electrochemistry to study dopaminergic modulation of connectivity dynamics and stress-driven repetitive behaviors.
May 9, 2025 at 10:31 AM
On Monday (May 12) morning at 8:15 in the multimodal e-poster session, Tatiana Shnitko will discuss the use of fMRI, electrophysiology, and electrochemistry to study dopaminergic modulation of connectivity dynamics and stress-driven repetitive behaviors.
On Monday (May 12) morning at 8:15 in the fMRI acquisition e-poster session, Sam Booth will demonstrate the use of PETALUTE for fMRI (collab with @uzayemir.bsky.social and Steve Sawiak), featuring a golden angle scheme to achieve flexible spatiotemporal resolution with multi-echo capability!
May 9, 2025 at 10:31 AM
On Monday (May 12) morning at 8:15 in the fMRI acquisition e-poster session, Sam Booth will demonstrate the use of PETALUTE for fMRI (collab with @uzayemir.bsky.social and Steve Sawiak), featuring a golden angle scheme to achieve flexible spatiotemporal resolution with multi-echo capability!
13/ The sequence, data, and scripts from this work are available with the manuscript and via camri.org/sordino/. We thank fMRI pioneers and those advancing zero- & ultra-short TE sequences for their inspiring work, and we hope this manuscript helps other labs adopt SORDINO!
March 17, 2025 at 4:19 PM
13/ The sequence, data, and scripts from this work are available with the manuscript and via camri.org/sordino/. We thank fMRI pioneers and those advancing zero- & ultra-short TE sequences for their inspiring work, and we hope this manuscript helps other labs adopt SORDINO!
12/ SORDINO for imaging glymphatic dynamics💧: Using Gd contrast, SORDINO enables brain-wide tracking of CSF influx without susceptibility artifacts. Its silent acquisition paves the way for studying glymphatic function in awake and sleeping mice – providing insights into brain waste clearance!
March 17, 2025 at 4:17 PM
12/ SORDINO for imaging glymphatic dynamics💧: Using Gd contrast, SORDINO enables brain-wide tracking of CSF influx without susceptibility artifacts. Its silent acquisition paves the way for studying glymphatic function in awake and sleeping mice – providing insights into brain waste clearance!
11/ SORDINO for cross-brain coupling during social behavior 🐭🐭: We developed a dual-mouse hyperscanning platform, enabling simultaneous fMRI of two interacting mice. SORDINO reveals synchronized inter-brain activity in DMN & SN, uncovering neural dynamics of social interactions!
March 17, 2025 at 4:17 PM
11/ SORDINO for cross-brain coupling during social behavior 🐭🐭: We developed a dual-mouse hyperscanning platform, enabling simultaneous fMRI of two interacting mice. SORDINO reveals synchronized inter-brain activity in DMN & SN, uncovering neural dynamics of social interactions!