4) The aberrant localization of PIP2 in the nucleus/nucleoli and the alteration of the nucleoli structure lead to changes in the accumulation of histone marks. For example, H4K20me1 decreases, which subsequently affects proper cell cycle progression.

3) What happens to cells when we interfere with PIP2 metabolism in the nucleus? In addition to preventing cell cycle progression, the structure of the nucleolus is affected and the localization of PIP2 to the Fibrillarin-labeled nucleoli region is altered.

2) We used our lab's gold standard chromatome protocol to study the oscillation of metabolic enzymes on chromatin during the cell cycle. We identified a correlation between cell cycle progression and PIP2 metabolism on chromatin.

1) By integrating FUCCI reporters and classical proliferation/senescent markers, we identified a population of cells, which we termed G0-senescent, that had never before been identified using FUCCI.

As promised, here is our NEW preprint on CELL CYCLE ASSOCIATED PIP2 NUCLEAR METABOLISM by our SUPER postdoc Toni Gañez, who knows all the secrets of FUCCI reporters 👇
www.biorxiv.org/content/10.1...

4) The aberrant localization of PIP2 in the nucleus/nucleoli and the alteration of the nucleoli structure lead to changes in the accumulation of histone marks. For example, H4K20me1 decreases, which subsequently affects proper cell cycle progression.

3) What happens to cells when we interfere with PIP2 metabolism in the nucleus? In addition to preventing cell cycle progression, the structure of the nucleolus is affected and the localization of PIP2 to the Fibrillarin-labeled nucleoli region is altered.

2) We used our lab's gold standard chromatome protocol to study the oscillation of metabolic enzymes on chromatin during the cell cycle. We identified a correlation between cell cycle progression and PIP2 metabolism on chromatin.

We adapted the FUCCI system and extensively characterized U2OS FUCCI using microscopy and FACS.
1) By integrating FUCCI reporters and classical proliferation/senescent markers, we identified a population of cells, which we termed G0-senescent, that had never before been identified using FUCCI.

Merry Christmas from the Sdelci Lab!

Stay tuned for our last preprint before the end of the year... lots of FUCCI colors 🎄-style and metabolic enzymes on chromatin during cell cycle progression
COMING SOON 💥

We made it among the BEST PICTURES OF THE YEAR by @natureportfolio.bsky.social
www.nature.com/immersive/d4...
Beautiful image representing in red the nuclear localization of MTHFD2 in pluripotent colon organoids by @NataliaPardoL
Find the manuscript here: www.nature.com/articles/s41...