Taking cue from ESCP25, tried the FAIMS std v low res and diff FAIMS voltages on Astral-Evosep zoom 80SPD using ionopticks Rapid 5x75 col. (DIANN2.0). CV -45V seemed to perform better for both 5ng and 250pg at precursor level. The story seemed a little different for CV-48V esp at lower 250pg conc..

#TeamMassSpec Curious on the average recovery observed across labs for SP3 sample prep.. seeing about ~50-60% of input (cell lyates by BCA pep est.) and found the following from recent literature (on FFPE).

pubs.acs.org/doi/10.1021/...

doi.org/10.1016/j.mc...

EMBL has a bunch of on-demand training courses and collection to choose @ebi.embl.org

www.ebi.ac.uk/training/on-...

Believe I came across this article here, but two things that stand out..
a. the proteome depth can perhaps be better for in-tip than transfer from Evo96 proteochip (?)
b. 10-12 dpp when keeping a shorter cycle time at 120SPD! (Diagonal or dia-PASEF) TimsUltra AIP

www.biorxiv.org/content/10.1...

Have tried on HT for 3 species mix.. did not come across any column issues. It was just few runs though..Perhaps it's do with column compatibility.. Thermo methods show the preferred columns..Bruker doesn't ...with regd to conc..Evosep shared the following for Zoom on Pro2..

Loving this protein specific peak area rel.abundance plots in Skyline..😄

"How much LSD?"

#Teammassspec any idea what's happening every 5min in this MS2 Scan time on Astral

It travelled across three continents, crossed two oceans over 2.0y, but finally done and is beautiful 😉

Wonder what's this great device that measures biomarker from a skin swab in 5 min and predicts skin health. Also, what's the biomarker🤔

If ever wondered about FDR for MBR, here's is an interesting discussion and a new tool for false discovery control on Peptide Identity Propagation (PIP)

Don't recall seeing this before..🤔

U937 Sc (n=22)..2/2
Protein counts vs cell diameter/ abundances/ data completeness

First runs from recently installed CellenOne
HeLa Sc/dda-lfq- Protein counts, abundances from consensus report(PD 2.5)/ data completeness (n=21) ..1/2