Roman Tsukanov
@romantsukanov.bsky.social
Researcher at the University of Göttingen, Germany. Microscopist, Method developer: super-resolution imaging, SMLM, DNA-PAINT, FLIM, Multiplexing, Microfluidics.
I would recommend changing tubing each (sensitive) experiment. In practice, in Exchange-PAINT workflow we change tubing only after multiple experiments. Of course, this requires tubing flushing after each experiment - we use ethanol 80% and then distilled water. It works great!
October 16, 2025 at 8:34 AM
I would recommend changing tubing each (sensitive) experiment. In practice, in Exchange-PAINT workflow we change tubing only after multiple experiments. Of course, this requires tubing flushing after each experiment - we use ethanol 80% and then distilled water. It works great!
as for tubing - Tygon is excellent: biocompatible, non-sticky and relatively cheap. We use it successfully for years even with sticky dyes. Of course once in a while we change tubing. Other tubing types should work as well - we did not try.
October 15, 2025 at 3:28 PM
as for tubing - Tygon is excellent: biocompatible, non-sticky and relatively cheap. We use it successfully for years even with sticky dyes. Of course once in a while we change tubing. Other tubing types should work as well - we did not try.
thanks for your interest! We use low pressure to push the fluid. When we stop the pressure (by closing the valve) the flow stops due to capillary forces. The pressure tubes have to be positioned on the same level as the sample to avoid gravitation-caused flow.
October 15, 2025 at 3:28 PM
thanks for your interest! We use low pressure to push the fluid. When we stop the pressure (by closing the valve) the flow stops due to capillary forces. The pressure tubes have to be positioned on the same level as the sample to avoid gravitation-caused flow.
Pumpy is great! In our implementation, we can bring the pressure tubes with fluids close to a sample. Typically, we used tubing length 10-15 cm, ensuring fast response and small dead volumes. Pictures will follow
October 15, 2025 at 3:16 PM
Pumpy is great! In our implementation, we can bring the pressure tubes with fluids close to a sample. Typically, we used tubing length 10-15 cm, ensuring fast response and small dead volumes. Pictures will follow
👏 Huge credit to Samrat Basak @samratb.bsky.social , Kim-Chi Vu, Nikos Mougios, and all authors!
Special thanks to Nazar Oleksiievets, Felipe Opazo @opazo.bsky.social, and Sören Brandenburg for their great support.
Special thanks to Nazar Oleksiievets, Felipe Opazo @opazo.bsky.social, and Sören Brandenburg for their great support.
October 15, 2025 at 12:29 PM
👏 Huge credit to Samrat Basak @samratb.bsky.social , Kim-Chi Vu, Nikos Mougios, and all authors!
Special thanks to Nazar Oleksiievets, Felipe Opazo @opazo.bsky.social, and Sören Brandenburg for their great support.
Special thanks to Nazar Oleksiievets, Felipe Opazo @opazo.bsky.social, and Sören Brandenburg for their great support.
Our air-driven system automates Exchange-PAINT workflows — flexible, modular, compatible with most SMLM setups, minimal dead volumes, and ready for smart automation.
Benchmarked with 5-plex imaging in U2OS cells and primary cardiomyocytes.
Benchmarked with 5-plex imaging in U2OS cells and primary cardiomyocytes.
October 15, 2025 at 12:29 PM
Our air-driven system automates Exchange-PAINT workflows — flexible, modular, compatible with most SMLM setups, minimal dead volumes, and ready for smart automation.
Benchmarked with 5-plex imaging in U2OS cells and primary cardiomyocytes.
Benchmarked with 5-plex imaging in U2OS cells and primary cardiomyocytes.
We showcase multiplexed 3D #SMLM imaging of microtubules, vimentin, and actin in U2OS cells 🧬💡
A massive collaborative effort - special thanks to Samrat Basak @samratb.bsky.social for driving the project!
#FLIM #FLPAINT #FLSMLM #MIETPAINT
#DNApaint #MIET #Cytoskeleton
A massive collaborative effort - special thanks to Samrat Basak @samratb.bsky.social for driving the project!
#FLIM #FLPAINT #FLSMLM #MIETPAINT
#DNApaint #MIET #Cytoskeleton
August 23, 2025 at 9:55 AM
We showcase multiplexed 3D #SMLM imaging of microtubules, vimentin, and actin in U2OS cells 🧬💡
A massive collaborative effort - special thanks to Samrat Basak @samratb.bsky.social for driving the project!
#FLIM #FLPAINT #FLSMLM #MIETPAINT
#DNApaint #MIET #Cytoskeleton
A massive collaborative effort - special thanks to Samrat Basak @samratb.bsky.social for driving the project!
#FLIM #FLPAINT #FLSMLM #MIETPAINT
#DNApaint #MIET #Cytoskeleton
It's a very targeted short review and hopefully is an easy read! Enjoy!! 🙂
February 1, 2025 at 4:09 PM
It's a very targeted short review and hopefully is an easy read! Enjoy!! 🙂
Thank you, Amir! Here is the correct link: www.degruyter.com/document/doi...
Advanced fluorescence lifetime-enhanced multiplexed nanoscopy of cells
In this review paper, we summarize the significant advancements in the field of fluorescence lifetime imaging microscopy (FLIM), particularly wide-field FLIM with single-molecule sensitivity, achieved...
www.degruyter.com
February 1, 2025 at 5:54 AM
Thank you, Amir! Here is the correct link: www.degruyter.com/document/doi...
In this work, we explored wide-field FL-SMLM and took an unconventional approach by combining a review with a detailed imaging protocol. It was particularly exciting to delve into the future prospective of novel wide-field FLIM technologies! 🔬
January 31, 2025 at 10:33 PM
In this work, we explored wide-field FL-SMLM and took an unconventional approach by combining a review with a detailed imaging protocol. It was particularly exciting to delve into the future prospective of novel wide-field FLIM technologies! 🔬