Our study provides a high-throughput platform and standardized genetic parts for chloroplast engineering, offering a foundation for rapid prototyping of complex genetic designs with potential transferability to crop plastids.

The best part: it’s freely available to the community ❤️

By integrating this to the MoClo standard, we enabled standardized, automated assembly and rapid testing of genetic combinations - resulting in more than 30 new synthetic genetic elements. We demonstrated a proof of concept by introducing a synthetic photorespiration pathway into the chloroplast!

We developed an automation workflow to generate, handle, and analyze thousands of transplastomic strains in parallel. We expanded the available selection markers and reporter genes and systematically characterized over 140 regulatory parts.

In our new study, we established Chlamydomonas reinhardtii as a prototyping chassis for chloroplast synthetic biology. This fast-growing alga has a single chloroplast per cell and can be cultivated in high-throughput formats, making it an ideal testbed.

The potential of chloroplast synthetic biology is immense: from increasing yield and resilience to improving nutrient profiles and developing new ways of CO₂ fixation. But realizing this potential requires more than traditional breeding or gene-editing techniques...

Happy birthday!

sorry for messing up the tag 😬 @olallalab.bsky.social

Thank you for being a part of [email protected] @bangebalcony.bsky.social @danielschindler.bsky.social @hoeferlab.bsky.social @schullerjm.bsky.social @georghochberg.bsky.social

Just in time for our kick off meeting - our homepage is ready! 🤩
Find all information on our research project between groups of @unimarburg.bsky.social and
@mpimicrobiomarburg.bsky.social, funded by #LOEWE: www.uni-marburg.de/en/fb17/robucop