Riddhiman Garge
@riddhiman.bsky.social
WRF Fellow w/ @jshendure.bsky.social & @leastarita.bsky.social | @uwgenome.bsky.social, @cegs-cmap.bsky.social & @brotmanbaty.bsky.social | PhD w/ @edwardmarcotte.bsky.social | Musician, vinyl collector & Man Utd fan outside the lab. Mostly in that order.
Very happy to share our latest led by the relentless @shawnfayer.bsky.social! We developed multiplexed assays to measure variant effects across diverse genetic and cell contexts in stem cell and differentiated cells. 🧵 👇
The effects of genetic variants primarily occur in differentiated cells meaning we need to access these cell types to measure variant effects for most disease genes. We developed saturation genome editing in stem cells (iPSC-SGE) to enable phenotyping in diverse genetic and cell contexts at scale!
Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contexts
Multiplexed assays of variant effect (MAVEs) systematically measure variant function but have been limited to cancer cell lines rather than disease-relevant cell types. We developed saturation genome ...
www.medrxiv.org
November 22, 2025 at 5:08 PM
Very happy to share our latest led by the relentless @shawnfayer.bsky.social! We developed multiplexed assays to measure variant effects across diverse genetic and cell contexts in stem cell and differentiated cells. 🧵 👇
Reposted by Riddhiman Garge
Congratulations to Karel Svoboda and @jshendure.bsky.social on their election to the National Academy of Medicine (@nam.edu)! Election to the Academy is one of the highest honors in health and medicine. #NAMmtg
More on their journeys to this achievement: alleninstitute.org/news/karel-s...
More on their journeys to this achievement: alleninstitute.org/news/karel-s...
Karel Svoboda and Jay Shendure elected to National Academy of Medicine
The honor recognizes leaders who have demonstrated outstanding achievement and made lasting contributions to the advancement of the medical sciences, health care, and public health
alleninstitute.org
October 20, 2025 at 7:15 PM
Congratulations to Karel Svoboda and @jshendure.bsky.social on their election to the National Academy of Medicine (@nam.edu)! Election to the Academy is one of the highest honors in health and medicine. #NAMmtg
More on their journeys to this achievement: alleninstitute.org/news/karel-s...
More on their journeys to this achievement: alleninstitute.org/news/karel-s...
Reposted by Riddhiman Garge
Super excited about first Shendure/Baker Lab collaboration & preprint on a multiplex sequencing-based strategy for screening de novo proteome editors in mammalian cells. Kudos to the brilliant Chase Suiter (not here) & @greenahn.bsky.social on the work! Preprint here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 14, 2025 at 6:45 PM
Super excited about first Shendure/Baker Lab collaboration & preprint on a multiplex sequencing-based strategy for screening de novo proteome editors in mammalian cells. Kudos to the brilliant Chase Suiter (not here) & @greenahn.bsky.social on the work! Preprint here:
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Riddhiman Garge
New paper from my lab and @jshendure.bsky.social lab! Led by the brilliant @zukailiu.bsky.social and @cxqiu.bsky.social. We tackled how anterior and posterior progenitor cells cooperate to self-organize into an embryonic structure (termed AP-gastruloid). (1/n) www.biorxiv.org/content/10.1...
September 26, 2025 at 6:06 PM
New paper from my lab and @jshendure.bsky.social lab! Led by the brilliant @zukailiu.bsky.social and @cxqiu.bsky.social. We tackled how anterior and posterior progenitor cells cooperate to self-organize into an embryonic structure (termed AP-gastruloid). (1/n) www.biorxiv.org/content/10.1...
Reposted by Riddhiman Garge
It’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.
February 8, 2025 at 9:21 PM
It’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.
Reposted by Riddhiman Garge
Now out in @science.org w/ @jshendure.bsky.social we present 'Genome-shuffle-seq': a method to shuffle mammalian genomes and characterize the impact of structural variants (SVs) with single-cell resolution in one experiment.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Multiplex generation and single-cell analysis of structural variants in mammalian genomes
Studying the functional consequences of structural variants (SVs) in mammalian genomes is challenging because (i) SVs arise much less commonly than single-nucleotide variants or small indels and (ii) ...
www.science.org
January 31, 2025 at 7:41 PM
Now out in @science.org w/ @jshendure.bsky.social we present 'Genome-shuffle-seq': a method to shuffle mammalian genomes and characterize the impact of structural variants (SVs) with single-cell resolution in one experiment.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Thanks GSA for blogging about our work!
Nice article about our brewing yeast proteomics paper with
@riddhiman.bsky.social and @edwardmarcotte.bsky.social! Beer brewers and geneticists collaborate on study of yeast changes during commercial fermentation genestogenomes.org/beer-brewers...
@riddhiman.bsky.social and @edwardmarcotte.bsky.social! Beer brewers and geneticists collaborate on study of yeast changes during commercial fermentation genestogenomes.org/beer-brewers...
Beer brewers and geneticists collaborate on study of yeast changes during commercial fermentation
Domesticated yeast adapts to harsh brewing tank environments, including little oxygen and lots of alcohol. While Riddhiman Garge, first author of a study published in the March 2024 issue of G3: Genes...
genestogenomes.org
May 10, 2024 at 8:09 PM
Thanks GSA for blogging about our work!
Reposted by Riddhiman Garge
How appropriate that our G3 paper with @riddhiman.bsky.social and @edwardmarcotte.bsky.social et al came out in final form while I'm at #TAGC24! Systematic profiling of ale yeast protein dynamics across fermentation and repitching academic.oup.com/g3journal/ar...
Systematic profiling of ale yeast protein dynamics across fermentation and repitching
Abstract. Studying the genetic and molecular characteristics of brewing yeast strains is crucial for understanding their domestication history and adaptations a
academic.oup.com
March 7, 2024 at 9:46 PM
How appropriate that our G3 paper with @riddhiman.bsky.social and @edwardmarcotte.bsky.social et al came out in final form while I'm at #TAGC24! Systematic profiling of ale yeast protein dynamics across fermentation and repitching academic.oup.com/g3journal/ar...
Very grateful to be amongst this amazing cohort of scientists! Many thanks to @wrfseattle for the opportunity to learn and grow in this diverse program. Finally, I can't thank my mentors @jshendure.bsky.social and @lea_starita at UW Genome Sciences enough for their support!
Washington Research Foundation announces its largest ever cohort of WRF Postdoctoral Fellows for 202...
www.wrfseattle.org
December 14, 2023 at 10:17 PM
Very grateful to be amongst this amazing cohort of scientists! Many thanks to @wrfseattle for the opportunity to learn and grow in this diverse program. Finally, I can't thank my mentors @jshendure.bsky.social and @lea_starita at UW Genome Sciences enough for their support!
Finally on BlueSky! Thrilled to share our work profiling Ale yeast protein dynamics across fermentation and serial repitching! biorxiv.org/content/10.1...
@maitreya.bsky.social @edwardmarcotte.bsky.social
@LiveOakBrewing #BeerYeast #SystemsBiology #Proteomics Sneek Peak🧵 below-
@maitreya.bsky.social @edwardmarcotte.bsky.social
@LiveOakBrewing #BeerYeast #SystemsBiology #Proteomics Sneek Peak🧵 below-
September 23, 2023 at 6:06 PM
Finally on BlueSky! Thrilled to share our work profiling Ale yeast protein dynamics across fermentation and serial repitching! biorxiv.org/content/10.1...
@maitreya.bsky.social @edwardmarcotte.bsky.social
@LiveOakBrewing #BeerYeast #SystemsBiology #Proteomics Sneek Peak🧵 below-
@maitreya.bsky.social @edwardmarcotte.bsky.social
@LiveOakBrewing #BeerYeast #SystemsBiology #Proteomics Sneek Peak🧵 below-
Reposted by Riddhiman Garge
Super fun project with @riddhimankg (twitter), @edwardmarcotte.bsky.social, @LiveOakBrewing (twitter), and friends! Systematic Profiling of Ale Yeast Protein Dynamics across Fermentation and Repitching
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
September 22, 2023 at 4:41 PM
Super fun project with @riddhimankg (twitter), @edwardmarcotte.bsky.social, @LiveOakBrewing (twitter), and friends! Systematic Profiling of Ale Yeast Protein Dynamics across Fermentation and Repitching
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...