📢 New preprint:
Experiment-guided AlphaFold3 resolves accurate protein ensembles.
doi.org/10.1101/2025...

AlphaFold3 is incredible, but has crucial limitations: it typically collapses to a single conformation, ignoring the inherent dynamics of proteins. And it can be wrong. Here's a solution. 🧵👇

Methyl-TROSY NMR is powerful to probe interactions, dynamics and structure in large molecule, but a significant bottleneck is the assignment of NMR signals to individual methyls.
We leverage a new isotope labeling scheme and a tailored NMR experiment to connect methyls of Leu and Val (->pic).

2/n

Interesting talk at ISMRM 2025 Hawaii about sustainable MRI equipment?

Some thoughts about the choice of location: people traveled 100 million km to get there, emitting 28000 tons of CO2.
Worth considering for a future conference (that may again include a sustainability talk) @ismrm.bsky.social

I have estimated the carbon emissions of the ISMRM conference 2024 in Honolulu.

Attending the conference emits about 4.9 tons/person on average. The location is far from the center-of-mass of the participants. Is this location really a good idea? Is it worth the damage?

@ismrm.bsky.social

Follow-up on our number-crunching of the CO2 footprint of #nmr conferences. The EUROMAR 2025, happening in lovely Northern Finland (Oulu) emits ~1.8 tons CO2 per person, ~2-fold higher than previous EUROMARs (around 1 ton). The choice of location matters.
It will surely be a great meeting. Enjoy.

How much CO2 is emitted, on average, when travelling to a conference? Here are the numbers, using the attendee lists of major #nmr conferences over the last ten years.

What if we reduced the carbon footprint of conferences by hundreds of tons of CO2 -- simply by choosing the locations more smartly? Here are our estimates/predictions for EUROMAR (#nmr) conferences.

Is train travel really emitting less CO2? After all, doesn't the construction and maintenance of the train infrastructure require a lot of energy & hence produce a lot of emissions? Here are our estimates -- including infrastructure emissions. Clearly: prefer trains over flights. (Details -> paper)

How much is the CO2 footprint of conference travel as compared to other research activities? This graph shows our estimates (single conference visit on the left, year-long research activity on the right (details in the paper).

5/
Methodology of my calculation:
I used the attendee list to estimate distance from affiliation to the conference site (distribution: see image).
Locals (California) assumed to drive. Others assumed to fly.
CO₂ calculations via CarbonTracer (Uni Graz)—a transparent, science-based tool.

3/
To compare:
The annual per-person CO₂ budget to stay under 2°C warming is ~3–4 tons.
One conference = more than half of that.
And: running a full NMR facility for a year (normalized to per-person level) emits probably less than a trip to the conference (see figure).

We have several PhD positions open to use NMR, cryo-EM and other tool to study dynamic proteins: chaperones, enzymes, mitochondrial import.
Please forward to interested candidates. Application deadline Jan 8 phd.pages.ist.ac.at
#chaperone #mitochondria #NMR #enzyme #allostery #PhDjobs #sciencejobs

Open #PhDposition: decipher the mechanisms underlying allostery.

What allows a protein to communicate over many nanometers to regulate its function? How did it evolve? What is the link to protein dynamics?
Join us to study exciting mechanisms by NMR & more.

More info attached.
phd.pages.ista.ac.at

🚨 PhD Positions: Mitochondrial Protein Import/Integrated Structural Biology

Find more info in the attached file or message me directly. I’ll get back to you as soon as I can!

🌟 Know someone perfect for this?
Please share post.

#PhDPositions #StructuralBiology #NMR #CryoEM #Mitochondria #Chaperone

This one influenced my choice of digging into mitochondrial protein import mechanisms.

🚨 Exciting PhD opportunity! IST Austria (Vienna)
Join us to uncover the mechanisms of mitochondrial protein import, or enzyme allostery. Use cutting-edge NMR & more. 🌟

📅 Apply by January 8
🔗 phd.pages.ista.ac.at/phd-applicat...

Full details attached! 💡 #PhDposition #StructuralBiology #ScienceJobs