We designed a DNA origami system that disassembles in presence of specific miRNA and visualised the disassembly process using high speed AFM. Find a link to the preprint in the comments. Feedback and comments are highly appreciated!!!

Exciting work led by @nano-chalmers.bsky.social delivered in collaboration with @georgerheath.bsky.social introducing a rationally designed DNA origami system for the multiplexed detection of miRNA in crude RNA extracts via nanopore sensing.

Working with Nikki Weckman (U toronto) and Emma Thomson (U Sheffield) 2e found out that a single point mutation in the guide RNA of the Cas9-RNP complex is sufficient to alter the digestion activities.

We use a polymer electrolyte as trans electrolyte which allows us to use biochemical buffers without compromising the detection sensitivity so we can monitor in real time enzymatic reactions

The TOC graphic was inspired from the signal analysis of the CP 1919 pulsar and Joy Division's Unknown Pleasures album. @ceesdekker.bsky.social ever publish a na-na-na nanopore album we have already got the cover sorted ! (those waves are DNA molecules translocating through the nanopore)