Wow! Cool! I have never thought about it that what did microbiologists use before discovering agar for culturing?maybe gelatin, but it is really hard to keep it solid!

Congrats!🎉

In my view, symbiosis is one of the ways of evolution; ‘cause Darwin’s law is not “competition only.” It’s about survival too, whoever survives and reproduces more wins.
If cooperation helps organisms survive or reproduce better, then natural selection will favor cooperation. That’s still Darwinian!

I can learn a lot from you Nikolas; I really hope to see you soon.

We have a lot of that trash data in ncbi, I suppose ncbi should have an option to flag a sequence as obsolete; something like we do in Linux arch repos, so maybe others know what they are dealing with. Bacteria is better in this regard, fungal taxonomy is like hell…actually many of them are useless.

Good luck Professor!

If I were you, I would compare my results with those of Dia et al. and the Morinière database; they have done extensive work on Xanthomonas classification, which you can find in the WGS repository of NCBI. And if your focus is Enterobacteriaceae, there is no research better than that of Pro. Brady.

you cannot compare your data to findings that have not been confirmed. recA works better in Enterobacteriaceae, but not in Xanthomonas. Even in Enterobacteriaceae, I would recommend using rpoB alongside gyrB.

Sorry for the late comment, Nikolas. I was working on some articles and had been away from social media. If I understand correctly, you are working on Xanthomonas. From my experience, recA is not a very reliable gene for intra-taxa definition. Also, please ignore all non-reported BLAST results.

Thanks; I haven’t stopped trying, even if it means starting again from the lowest level. One day, like the ugly duckling, I’ll grow into a swan. Maybe right now I’m just another Middle Easterner people don’t look at kindly, but a good day will come eventually. Hope so. Thank you for reading me 🙏🏼

😍

Wow! Incredible! 😍

I’d honestly love to; sounds like a dream. I just don’t know whom I should contact or where to start. And Brazil? Who doesn’t love Brazil! Thank you so much for mentioning it. I’ll bother you in private if that’s okay 🙏

Thank you for reading me. I’ve tried, waited, and held on as much as I could… and I still do. Funny enough, my name — Omid — actually means hope. Maybe that’s what keeps me going. Truly appreciate your kind words 🙏

Born in the wrong time. Living in the wrong world…

Congrats! Once I had the same problem and it got resolved when I manually made the fresh buffer (TBE) myself.